The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase
A Level Biology Project
This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase.
This is a real A-level school project and as such is intended for educational or research purposes only. Extracts of this project must not be included in any projects that you submit for marking. Doing this could lead to being disqualified from all the subjects that you are taking. You have been warned. If you want more help with doing your biology practicals then have a look at 'Advanced Level Practical Work for Biology' by Sally Morgan. If you want more detailed biology information then I'd recommend the book 'Advanced Biology' by M. Kent.
Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all very specific as each enzyme just performs one particular reaction.
Catalase is an enzyme found in food such as potato and liver. It is used for removing Hydrogen Peroxide from the cells. Hydrogen Peroxide is the poisonous by-product of metabolism. Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown in the equations below.
Hydrogen Peroxide---------------------->Water + Oxygen
It is able to speed up the decomposition of Hydrogen Peroxide because the ...
... middle of paper ...
...ental error involving one of the factors mentioned above.
This experiment could be improved in a number of ways. It could be repeated more times to help get rid of any anomalies. A better overall result would be obtained by repeating the experiment more times because any errors in one experiment should be compensated for by the other experiments.
Using more concentrations of Hydrogen Peroxide would have produced a better looking graph and I would have liked to use concentrations higher than 20% to extend the graph so that the maximum possible rate of reaction could be reached.
The problem of the delay between pouring in the Hydrogen Peroxide, bunging the test tube and starting the stopwatch could have been limited by getting another person to start the stopwatch when the hydrogen peroxide was poured into the tube.
Other Biology Projects
Need Writing Help?
Get feedback on grammar, clarity, concision and logic instantly.Check your paper »
- The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Introduction This is a real A-level school project and as such is intended for educational or research purposes only. Extracts of this project must not be included in any projects that you submit for marking. Doing this could lead to being disqualified from all the subjects that you are taking.... [tags: biology experiment, chemistry experiment]
2003 words (5.7 pages)
- The Effect of Substrate Concentration on the Rate of Activity of the Enzyme Catalase Background Information: Enzymes are biological catalysts that increase the speed of chemical reactions without undergoing any physical change. They are neither used up in the reaction nor do they appear as reaction products. All enzyme catalysed reactions are reversible. Enzymes effectively lower the amount of activation energy required for chemical reactions to start. Some enzymes weaken covalent bonds within the substrate molecule, whereas in other cases this lowering of the activation energy seems to take place because the enzyme holds the substrate molecules in a particular p... [tags: Papers]
3145 words (9 pages)
- The Effects of Enzyme Concentration on the Activity of Amylase To investigate the effect of Amylase concentration on its activity. the relative activity of Amylase is found by noting the time taken for the starch substrate to be broken down, that is, when it is no longer gives a blue-black colour when tested with iodine solution. This time is referred to as the achromatic point. Equipment: v Amylase solution 0.1% v Starch Solution 1.0% v Distilled water v Iodine in potassium iodine solution v White tile and polythene pipette v Graduate pipettes or syringes v Test tubes in rack v Beaker (used as water bath) v Stopwatch, Thermometer v Eye P... [tags: Papers]
986 words (2.8 pages)
- The Effect of pH on Enzyme Activity pH is a measure of the concentration of hydrogen ions in a solution. The higher the hydrogen ion concentration, the lower the pH. Most enzymes function efficiently over a narrow pH range. A change in pH above or below this range reduces the rate of enzyme reaction considerably. Changes in pH lead to the breaking of the ionic bonds that hold the tertiary structure of the enzyme in place. The enzyme begins to lose its functional shape, particularly the shape of the active site, such that the substrate will no longer fit into it, the enzyme is said to be denatured.... [tags: Papers]
602 words (1.7 pages)
- Enzymes are biological macromolecule that acts as catalysts and increase the rate of a chemical reaction. Without enzymes, life, as we know about it, would not exist. Enzymes function by deceasing the activation energy and stabilizing the transition state of a chemical reaction without altering the thermodynamic of reaction (#1 Boyer). At the molecular level, enzymes catalyze these reactions by binding to the substrate or reactants to form an enzyme-substrate complex. The reaction takes place while the substrate is bound to the enzyme and converting the substrate to the new product.... [tags: Enzyme, Enzyme inhibitor, Catalysis]
947 words (2.7 pages)
- The Effect of Varying Environmental Factors on Catalase Activity I. Introduction A. Background Proteins are one of the main building blocks of the body. They are required for the structure, function, and regulation of the body’s tissues and organs. Even smaller units create proteins; these are called amino acids. There are twenty different types of amino acids, and all twenty are configured in many different chains and sequences, producing differing protein structures and functions. An enzyme is a specialized protein that participates in chemical reactions where they serve as catalysts to speed up said reactions, or reduce the energy of activation, noted as Ea (Mader & Windelspecht).... [tags: Enzyme, PH, Catalysis, Active site]
1747 words (5 pages)
- Effects of Modifying Concentration, pH , and Boiling on Activity of Peroxidase Abstract An enzyme is a catalysis and catalysis s substance that increases the rate of a chemical reaction without itself going through a permanent chemical change. In this lab we will discover exactly how the substrate connects with the active site. The main substance we use throughout this lab is peroxidase a eukaryotic organelle from plant tissues. Once there is a color change we test that using a spectrophotometer.... [tags: Enzyme, PH, Buffer solution, Temperature]
1925 words (5.5 pages)
- Activity of the Enzyme Catalase with Hydrogen Peroxide Background Information: An enzyme (Catalase) is a biological catalyst. It can modify the rate of reaction without being changed itself. Enzymes are also globular proteins that have a three-dimensional specific shape; among with a pocket known as the active site, this has a precise shape to link with the exact substrate. Every enzyme are capable of converting one kind of substrate molecule into one kind of product molecule, which forms the enzyme-substrate complex.... [tags: Papers]
2153 words (6.2 pages)
- The purpose of this experiment was to see if there would be enzyme activity that is in catalase, which is found in potatoes. How my partner and me did the experiment was very simple, we put the filter paper filled with catalase concentration in to five sets of hydrogen peroxide. We found that the more concentration of hydrogen peroxide there was the faster it would take for the filter paper to reach the top, and the lower to no concentration level of hydrogen peroxide was the slower it would go to reach the top.... [tags: catalyst, potatoes, experiment]
1803 words (5.2 pages)
- Investigating Enzyme Activity Aim: To investigate how the concentration of hydrogen peroxide effects the rate of reaction of an enzyme (catalase) Variables: These factors could effect the rate of reaction on an enzyme: · pH · Concentration · Temperature · Surface Area pH - Enzymes function at different pH values. In neutral conditions the amount of oxygen gas given of in an enzyme-catalysed reaction will increase. An enzyme is affected by how much acid or alkali is present.... [tags: Papers]
3981 words (11.4 pages)