Effect of pH and Acid Phosphatase Essay

Effect of pH and Acid Phosphatase Essay

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The practical was carried out to investigate the effect of pH on the reaction of the enzyme acid phosphatase.

Of the many functions of proteins, catalysis is by far the most vital. When catalysis is not present, most reactions in the biological systems take place very slowly to produce at an adequate pace for metabolising organism. The catalysts that take this role are called enzymes. Enzymes are the most efficient catalysts; they can enhance rate of reaction by up to 1020 over uncatalysed reactions. (Campbell et al, 2012).

Enzyme catalysis is dependant upon factors such as concentration of enzyme and substrate, temperature and pH. These factors determine the rate of reaction, and an increase in temperature or pH above the optimum will lead to the denaturation of the enzyme and a decrease in the rate of reaction. There are many phosphatase enzymes but they are classified as those with alkaline and acid pH optimum. Both catalyse general reaction: ROPO3H2+ H2O –ROH + HPO42-+2H+.

Each enzymes works with a small range of pH, there is a pH at which its activity is greatest called optimal pH. This is due to the changes in pH can make a break intra and intermolecular bonds, distorting the shape of the enzyme, and its effectiveness. Generally, enzymes have an optimum pH this doesn’t go to say that the optimum is the same for each enzyme. For example the optimum pH for enzyme pepsin found acidic lumen in the stomach is lower than that of the enzyme carbonic anhydrase that works in the cytosol at neural pH.

Predominantly, enzymes show specificity for phosphatase monoesters but are relatively non-specific to (RO-). This can be used to construct an assay that uses non-physiological substrate, p-nitrophenyl phosphate, and one that has a p...


... middle of paper ...


...testing) would enable a clear distinction in determining the variation in the data. However, the mean can show a simple interpretation of a distorted view of distribution on occasions (outliers). Standard Deviation gives the right picture, smaller the standard deviation higher the central tendency and data is concentrated around the mean. Higher value of standard deviation indicates better distribution of data. Thus, a specific read on the optimum pH can be identified.


Word count = 1599

References

Campbell, M.K., Shawn, S.O. (2012). Biochemistry, 7th Edition. The Behaviour of Proteins: Enzymes. Pg 139-159.
Van Etten, R.L. and Waymack, P.P. (1991). Substrate specificity and pH dependence of homogeneous wheat germ acid phosphatase. Archives of Biochemistry and Biophysics 288, 634.
Walsh, C. (1979) Enzymatic Reaction Mechanisms, W.H. Freeman, San Francisco.



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