The Effect Of Light Intensity, Nh4cl, And Dcmu On The Rate Of Electron Transport

The Effect Of Light Intensity, Nh4cl, And Dcmu On The Rate Of Electron Transport

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Investigating the effects of light intensity, NH4CL, and DCMU on the rate of electron transport in spinach thylakoid membranes was the purpose of this lab. Photosynthesis is the process that routinely drives electron transport across the thylakoid membrane but can be impacted when additional factors are added. Two phases known as Light reactions and the Calvin cycle makeup photosynthesis. Plastoquinone shuttle, water oxidation, and NADP+ reduction are the major factors of the light reactions. Photosynthesis creates a proton gradient when pigments in the thylakoid membrane absorbs light energy from the sun. The energy is then used to oxidize water which is the initial electron donor, and NADP+ acts as the final electron acceptor. Protons then flow downhill from the lumen to the stroma through ATPase in the thylakoid membrane to form ATP, and NADPH is formed from electron transport. ATP and NADPH from the light reactions drive the Calvin cycle which takes place in the stroma. A sugar known as GAP is the product of the Calvin cycle and is converted to sucrose in the cytosol. In our experiment DCMU is an herbicide that impedes electron transport between photosystemII and plastoquinone. NH4CL is an uncoupler which diminishes the proton gradient and allows electrons to by ATPase. In regard to light intensity for our lab, it was predicted that less light will slow down electron transport. We also predicted that DCMU will decrease the rate of electron transport, and NH4CL will increase the rate. It was hypothesized that if NH4CL allows protons to bypass ATPase then the rate of electron transport will increase.
Materials and methods:
Isolated spinach thylakoid membranes were used for our lab to observe the rate of electron transport. Th...


... middle of paper ...


...ctron transport between photosystemII and plastoquinone. Plastoquinone is the first agent that shuttles electrons but DCMU stops it from receiving the electrons. Since plastoquinone does not receive the electrons the transport of electrons is slowed down and less ATP and NADPH are produced. NH4CL is acted as an uncoupler in the lab. Uncouplers destroy the proton gradient, and allow protons to pass freely bypassing ATPase. ATP production stops but electron transport continues. Since there is no gradient electrons move faster because there is less resistance due to ATP production being ceased. The information obtained from this lab is utilized in everyday life and we may not even n notice it. Uncoupling of NH4CL is the reason why animals dislike the smell of ammonia. It is also the reason NH4CL must be absorb quickly into amino acids when used as a plant fertilizer.


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