Essay The Effect Of Emulsification On The Digestion Of Lipids

Essay The Effect Of Emulsification On The Digestion Of Lipids

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In part A of the lab, our group measured the effects of emulsification on the digestion of lipids in the presence of cholic acid, a purified bile salt, and distilled water. The tube containing vegetable oil and no bile began to separate into two layers within the first minute of being mixed together. Although there were no clear distinctions within the first 15 seconds, by the fifth minute, there appeared two separate layers; one resulted in a yellow appearance while the other one was clear. As expected and predicted in our hypothesis, it was easier to notice the separation of the two layers in the tube without any bile salts because lipids are hydrophobic meaning that oils are more difficult to digest. However, due to the fact that when in the presence of bile salts, lipids breakdown into smaller particles, our hypothesis stated that the second tube with cholic acid would not separate into two layers as the solutions in the first tube; rather, it would mix and emulsify. Our results were very similar to our hypothesis because the bile salts mixed and were able to stay together longer due to emulsification. In the second part of Part A, we observed the effect lipid digestion has on pH levels. We predicted that the first tube with bile would become yellow as the pH dropped. Since bile and lipid combined allow for both to mix and to digest, there is an increase in surface area, which allows lipase to break down fats. The breakdown of these fats decreases the pH levels allowing the solution to turn yellow. The second tube did not have bile so we assumed that there would be little to no emulsification of bile salts making the solution’s pH higher. However, after 45 minutes of being incubated, the solution appeared yellow. This is most...


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... the reactions of an intact and chopped potato. The cutting and mincing of food will help increase this surface area and expose more food to be digested; hence, it is important to properly chew food instead of rushing while eating.
3. If I were to redo the experiment, I would carefully make sure everything is accurately measured and properly cut as a way to get the most effective results. I would also attempt to participate in all the different parts of the lab to get a better understanding of the experiment instead of simply collecting the data from my classmates. By being a part of each section of the lab, regardless of its time consumption, I would have a better insight of the process and outcomes of the each test. But, because there was a time limit, there were moments of uncertainty of what my group members were doing and I was not able to get a clarification.

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