The Effect Of Acid Concentration On The Enzyme Activity Of The Alkaline Phosphatase Enzyme

The Effect Of Acid Concentration On The Enzyme Activity Of The Alkaline Phosphatase Enzyme

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Enzyme activity can be effected by a series of factors such as pH, temperature, and concentration of enzyme (Campbell, 2014). The purpose of this experiment is to determine the specific effects due to the change in pH, temperature, and concentration on the enzyme activity of the Alkaline Phosphatase Enzyme. The results displayed the effects of the Alkaline Phosphatase Enzyme due to the shifts in environment. The temperature shift revealed the enzyme’s optimal temperature for it’s to function ideally. The concentration increase within the enzyme resulted in the increase in enzyme activity. The pH levels showed that the Alkaline Phosphatase Enzyme was most ideal under neutral conditions. The ideal functioning of the Alkaline Phosphatase Enzyme in neutral pH levels is associated with how the enzyme is present within cells and extracellular fluid matrices with similar environments. The specific increase in enzyme activity due to temperature and concentration is correlated to the rate of reaction theory. In overall, the experiment remain parallel with the theories presented with rate of reaction and enzymes.
II. Introduction
A catalyst is a chemical agent that contributes to the speeding up of reactions without changing or being consumed by the reaction. One example of a catalyst are enzymes which are proteins that assist in speeding up reactions (Campbell, 2014). A reaction is sped up through an enzyme lowering or eliminating the activation energy barrier of the reactants. This results in the reactants not requiring as much energy without an enzyme resulting in the reaction to be able to occur more easily and quickly (Campbell, 2014). The enzyme binds with a substrate also known as the reactant within the reaction it at the active si...


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...e increase in pH will have effect the enzyme activity of the Alkaline Phosphatase Enzyme. The null of this hypothesis is that the increase in pH will not have effect the enzyme activity of the Alkaline Phosphatase Enzyme. It is also hypothesizes that the increase in concentration of the alkaline phosphatase enzyme will increase the enzyme activity of the Alkaline Phosphatase Enzyme. The null hypothesis for the concentration is that the increase in concentration of the alkaline phosphatase enzyme will not increase the enzyme activity of ALP. For the temperature, it is hypothesize that the increase in the temperature of the alkaline phosphatase enzyme will increase the catalytic rate of the Alkaline Phosphatase Enzyme. The null hypothesis for the temperature is that the increase in the temperature will not increase the catalytic rate of the Alkaline Phosphatase Enzyme.

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