In other words, homogenization holds mixture of disrupted cells at a series of increasing speeds. At each speed, the resulting force causes a fraction of the cell components to settle to the bottom of the tube, forming a pellet. At lower speeds, the pellet consists of large components and higher speeds yield a pellet with smaller (Reece. et. al., 2014)
Bivalve molluse (oyster) can be homogenized by physical and chemical method. Physical to use for oyster are chopping, and grinding. Potential sources of physical damage include the denaturation of proteins, RNA and DNA upon exposure to a different temperature and also damage caused by the homogenization technique. Chemical methods are osmatic chemical lysis, sonication or freeze-thaw cycles. Potential sources of chemical damage include: the degradation of proteins, RNA and DNA by proteases, RNAse and DNAse respectively that are released from the tissue when it is homogenized.
Selective excision of oyster tissue, the...
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.... (Table 2). In reduction, DCPIP decolorized from blue colour and reaction is followed by spectrophotometry (Figure 2). However, DCPIP presents shortcomings time and colour intensity (absorbance) are linearly related for only a very short period of time. After, time and absorbance were decreasing rather than increasing absorbance because solution of substrate is unstable. (Munujos et. al.,1993).
The advantage of cell fractionation enables to localize organelles, components, and metabolic pathway. Researchers prepare specific cell in bulk and identify their function. For example, biochemical tests shows the presence of SDH activity enzyme involved in cellular respiration, while electron microscopy revealed large numbers of the organelles called mitochondria. These data helped together that mitochondria are the sites of cellular respiration. (Reece. et. al., 2014)
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