Determining Vmax and Km of Alkaline Phosphatase

1. Introduction

1.1. Aim

1.2. Theory and principles

1.3. Application of principles

2. Experimental

2.1. Table of list of materials

2.2. List of apparatus

2.3. Procedure

3. Data and calculations

3.1. Record of all relevant raw data

3.2. Calculations with statistical manipulations

4. Results and discussion

4.1. Interpretation of data and comparison of results with known values

4.2. Discussion of the significance of the results

4.3. Was the original aim achieved

4.4. Conclusion

5. References

1. Introduction

1.1. Aim

To determine the kinetic parameters known as Vmax and Km of Alkaline Phosphatase. This will be done by determining and investigating the optimum pH and temperature at which Alkaline Phosphatase function.

1.2. Theory and principles

Enzymes are the most efficient catalysts known. (1) Enzymes are biomolecules that catalyze chemical reactions by decreasing the Activation Energy (the minimum energy required in a chemical system in order for a chemical reaction to take place), which in turn increases the rate of the chemical reaction. The catalyst itself does not change or is not consumed in the reactions they catalyze. Enzymatic reactions consist of substrates and products. According to the Oxford Dictionary a substrate is “the substance on which an enzyme acts” and a product is “ a substance produced during a natural or chemical process”. (2) In general, all chemical reactions require enzymes in order to occur at an acceptable tempo for life in biological systems. Enzymes are very substrate-specific. This specificity of the enzyme molecule is due to the complementary shape of the active site of the protein and the substrate. An enzyme consists of two p...

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...s vs. pH on x-axis, reaction rate at which pH was at its maximum could be determined.

4.2. Discussion of the significance of the results

4.3. Was the original aim achieved

4.4. Conclusion

5. References

Works Cited

1. Campbell MK, Farrell SO. Biochemistry. 7th ed.: Brooks/Cole Cengage Learning; 2012.

2. Oxford Dictionaries. Oxford Dictionaries. [Online].; 2014 [cited 2014 April 19. Available from: www.oxforddictionaries.com/definition/english/substrate.

3. Mason KA, Losos JB, Singer SR. Biology. 10th ed. New York: Mc Graw Hill; 2014.

4. AG Scientific. AG Scientific. [Online].; 2014 [cited 2014 April 19. Available from: www.agscientific.com/molecular-biology/molecular-biology.html.

5. International Union of Biochemistry and Molecular Biology. [Online].; 2002 [cited 2014 April 21. Available from: onlinelibrary.wiley.com/store.