CRISPR-Cas9 was used to edit the ade6 gene in Schizosaccharomyces pombe (S. pombe). By using sgRNA directed against the gene of interest, we calculated the mutagenesis efficiency of the colonies in plate pMZ288 is 25.7%. In addition, we used gel electrophoresis to determine the size of DNA fragments. We observed the cell contains wildtype ade6 digested with XhoI cut site produced 2 bands of PCR product, whereas mutant ade6 produced 1 band PCR product due to the lack of XhoI cut site.
To resolve the size of DNA fragments, we use gel electrophoresis that involves agarose gel and el...
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