Essay on CUMS-Induced Increase in ERα/ERβ Expression Part 2

Essay on CUMS-Induced Increase in ERα/ERβ Expression Part 2

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3.1 CUMS increased anxiety- and depression-related behaviour
Mice display distinct behavioural changes, suggesting an anxiety or depression response after exposure to chronic stress. Therefore, the behavioural response of control and CUMS mice in an anxiogenic environment was investigated using EPM and OFT and were compared with control mice. In EPM test, CUMS mice exhibited a significant (P<0.05; Student’s t test) reduction in both percentage time in open-arm (Control: 21.6 + 3.1%, n=10; CUMS: 12.2 + 3.3%, n=10; Fig. 2A) and percentage open-arm entries (Control: 40.9 + 3.5 %, n=10; CUMS: 24.6 + 4.2%, n=10; Fig. 2B). Thus, the CUMS mice made fewer entries and spent less time in open arms of the maze than control mice, indicating an enhanced anxiety response. Similar to the results in EPM, we observed increased anxiety response in OFT (Fig. 2C). In addition to measuring the number of entries in the central zone, we also analysed changes in the number of leaning, rearing, grooming and defecation of CUMS mice as compared to control. We observed a significant reduction in the number of entries in the central zone of the open-field (Control: 15.8 + 1.8, n=10; CUMS: 9.1 + 2.2, n=10) and rearing behaviour (Control: 9.6 + 0.9, n=10; CUMS: 6.3 + 1.5, n=10). Furthermore, as compared to control mice, CUMS exposure significantly increased grooming (Control: 2.3 + 1.3, n=10; CUMS: 4.8 + 0.7, n=10) and defecation (Control: 1.3 + 0.4, n=10; CUMS: 3.6 + 0.5, n=10) in young male mice. There were no overall significant differences between the control and CUMS mice with regard to leaning behaviour.
The behavioural effects of CUMS were further analysed by FST and sucrose preference test. When tested in FST (Fig. 2D), CUMS mice showed significant incr...

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...s in ovariectomized mice submitted to chronic unpredictable stress. Behav Brain Res 2012;227:287-290.

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[23] Hojo Y, Hattori TA, Enami T, Furukawa A, Suzuki K, Ishii HT, Mukai H, Morrison JH, Janssen WG, Kominami S, Harada N, Kimoto T, Kawato S. Adult male rat hippocampus synthesizes estradiol from pregnenolone by cytochromes P45017alpha and P450 aromatase localized in neurons. Proc Natl Acad Sci U S A 2004;101:865-870.

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