Composition of amino acids in strawberry fruits determined by gas chromatography with flame ionization under the influence of CO, NOx and SO2

Composition of amino acids in strawberry fruits determined by gas chromatography with flame ionization under the influence of CO, NOx and SO2

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Introduction
Amino acids are present in almost all foods in different relative items such as fruit juices, syrups, jams, jellies, wines etc. (Silva et al. 2005; Hua et al. 2014). In plant materials the amino acids are found in free form (L-configuration). Among the highly consumed fruits strawberries are widely used throughout the world for different purposes such as preparation of jams, jellies, decorating cakes etc. Due to high consumption, strawberry fruits are widely grown throughout the year irrespective of temperature, light and humidity in green houses by maintaining the temperature due to direct heating system or CO2 fertilizer units. The use of the CO2 unit/direct heating system has been used in many countries for photosynthetic efficiency. Although the CO2 fertilizer units/direct heating system are very much influenced for high yield of fruits or seeds in different crops whereas, the use of these CO2 fertilizers often lead to incomplete combustion and results in production of hazardous gases (Guo et al. 2007) such as carbon monoxide (CO), nitroxide (NOx) and sulfur-dioxide (SO2) (Muneer et al. 2014) which are harmful for plants by producing reactive oxygen species (ROS) and decreasing photosynthesis. It is estimated that up to 12 ppm of these gases are not potential threat (Adgate et al. 2014) however, if the concentration goes beyond that, it will potentially cause severe loss in crop productivity.
Numerous chromatographic methods are available so far for determination of free amino acids (Fountoulakis and Lahm 1998; Molna´r-Perl, 2000; Haberhauer-Troyer et al. 2003; Kugler et al. 2006; Guo et al. 2013). The method employing alkylchlorformate derivatization, solid phase extraction (SPE), and gas chromatography-flame i...


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...saas a hyperaccumulator. Redox Biol 2:91-98
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