Summary The importance of calcium as a second messenger probably can’t be emphasized enough. Ca2+-signalling through Ca2+-sensors such as calmodulin regulates a variety of vital functions in cells in response to stimuli. Calmodulin is a small evolutionally conserved Ca2+-binding pro-tein of the EF-hand superfamily that participates in many different cellular pathways through regulation of a diverse group of targets. Its function has shown to depend hugely on the way it is distributed throughout the cell and pools probably allow for CaM to be activated at the right time and place. Gene organization and evolutionary history The three genes encoding human Calmodulin, CALM 1-3, are non-allelic and thus localized on different chromosomes, 14q24-q31, 2p21.1-p21.3 and 19q13.2-q13.3 respectively [1]. These CaM-genes show identical intron-exon boundaries, consist of six exons and code for an identi-cal monomeric CaM-protein of 148 amino acids (fig. 1), but transcribes to at least 7 different major mRNAs differentiating relative to alternative polyadenylation among transcripts of each gene [2]. In addition to the three known CALM-genes the human family of CaM/CaM-like genes holds numerous pseudogenes and calmodulin-like proteins [1]. CaM is a highly evolutionally conserved protein found in all eukaryotic cells (fig. 2). Since CaM belongs to the superfamily of EF-hand proteins (fig. 1) its main structure originated before the branching into plants, fungi, invertebrates and vertebrates. It’s probable that this happened as two gene duplications followed by fusion led to the joining of four EF-hands into one struc-ture. Further modifications that account for the invariant CaM in vertebrates [2] and later the three bona fide CaM-genes of mamma... ... middle of paper ... ...n of Long-Term Potentiation [12]Dynamic redistribution of calmodulin in HeLa cells during cell division as revealed by a GFP-calmodulin fusion protein technique [13] Novel aspects of calmodulin target recognition and activation [14] Solution NMR Structure of Apo-Calmodulin in Complex with the IQ Motif of Human Car-diac Sodium Channel NaV1.5 [15]Modulation of Calmodulin Plasticity in Molecular Recognitionon the Basis of X-ray Struc-ture [16] Structural Basis for Calmodulin as a Dynamic Calcium Sensor [17] Tyrosine phosphorylation modulates the interaction of calmodulin with its target pro-teins [18] Berchtold, M. W. and Villalobo, A. (2013): The many faces of calmodulin in cell prolifera-tion, programmed cell death, autophagy, and cancer [19] The alterations of Ca2+/calmodulin/CaMKII/CaV1.2 signaling in experimental models of Alzheimer's disease and vascular dementia
...ve stress [8] and can help combat many cardiovascular disorders, thus confirming the protective capabilities of nitric oxide. Another one of nitric oxide’s protective capabilities stems from its role as a potent vasodilator, and these vasodilation properties are primarily achieved through the activity of eNOS. eNOS is a membrane bound protein that is classified as a calcium-calmodulin dependent enzyme [13]. At resting concentrations of calcium, eNOS generates low amounts of nitric oxide, however, as intracellular calcium concentration increases, there is a potent enhancement of nitric oxide production by eNOS [13], which then goes on to cause vasodilation to prevent blockage of blood flow. Due to its generally low output, eNOS is largely associated with basal cardiac function, while iNOS is a much higher output enzyme, generating much more nitric oxide than eNOS [5].
Voltage-gated calcium (Ca2+) channels are cellular membrane proteins that are important for electrical and chemical signaling in cells (5). In neurons voltage-gated Ca2+ chan...
calcium channels will help scientists to discover the precise cause and effect of LES which will in turn
Different cellular signaling pathways respond to calcium levels. The inflow of calcium resulting from glutamate receptor stimulation leads to their activation.
...on and forms an inhibitory complex with caveolin-1 leads to decrease in activity of enzyme in the cells. Transcription of Cav-1 gene is regulated by cholesterol responsive elements. Exposure of fibroblast and endothelial cells to free cholesterol and LDL Cholesterol was found to up regulate Cav-1 expression. Ca+2 mobilizing agents cause disinhibiton of e NOS by promoting Ca+2/Calmodulin triggered dissociation of Cav-1.
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Scientists know that Alzheimer disease is characterized by a gradual spread of sticky plaques and clumps of tangled fibers that disrupt the organization of nerve cells in the brain. However , a definite cause, prevention, or cause has not been found.
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Cellular membranes are complex mixtures of proteins and lipids. Cell membranes are composed of a phospholipid bilayer, consists of two leaflets of phospholipid molecules and their fatty acid chain form the hydrophobic interior of the membrane bilayer; and proteins that span the bilayer and/or interact with the lipids on either side of the two leaflets. Transmembrane proteins are the type of membrane proteins which span the entire length of the cell membrane. They are embedded between the phospholipids and provides a channel through which molecules and ions can pass into the cell. They enable communication between cells by interacting with chemical messengers. Membrane proteins were classified into two comprehensive categories- integral and
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