bone

1003 Words3 Pages

Over the 6-week experimental period, food intake and initial body weight were the same in all groups (Table 1). However by the end of the 6th week, the final body weight increased in all five groups of mice. The incremental increase in weight, however, was larger in the STD group (21.35 ± 0.87 to 25.01 ± 0.14 g). Alternatively, weight gain in the STD and SHAM groups was higher (3.66 ± 0.37 and 3.38 ± 0.30 g, respectively) (Table 1). The results in Table 1 show that glucose levels decreased in all mice independently of the diet but there was a significant decrease in mice fed with fructans over the course of the experiment.

Fecal excretion

In general, groups fed diets supplemented with fructans showed a tendency increase fecal excretion compared to the STD group (CAF1 = 9.31 ± 0.12 g, CFA2 = 10.35 ± 0.15 g, RNE = 8.84 ± 0.11 g, STD = 7.34 ± 0.23 g, and SHAM = 8.34 ± 0.12 g) (Table 1). However the increment was not significant.

Short Chain Fatty Acids

With respect to SCFA concentration in the caecum, the groups fed diets supplemented with fructans (CAF 1, CAF 2, and RNE) showed increased SCFA content compared to the standard diet groups (STD, SHAM). However, the CAF1 group had the highest concentration of SCFAs (Table 2) and acetate and butyrate in the caecum (34.72 ± 1.01 mmol/Kg and 18.67 ± 2.13 mmol/Kg, respectively). CAF2 had the highest concentration of propionate (25.64±1.03 mmol/Kg). The concentration of SCFAs in the colon showed the same trend observed for the caecum where CAF1 had a greater rise in total concentration of SCFAs (Table 2) and in the individual concentrations of acetate and butyrate (44.03 ± 1.91 mmol/Kg and 16.21 ± 2.00 mmol/Kg, respectively).

Calcium and Magnesium Analysis

Calcium content in plasma (...

... middle of paper ...

...sh differences between the groups fed diets supplemented with the different sources of fructans.

Microstructural analyses showed that the groups taking CAF1 and CAF2 fructans displayed low porosity (8.66 and 8.56 µm, respectively), whereas the STD group displayed higher porosity (10.75 µm). The same results were observed with regard to trabeculae diameter. Specifically, the standard group’s trabeculae had smaller diameters compared to the Agave fructan treated groups (Fig. 6).

Thickness of the compact bone was also affected by the ovariectomy. The STD group presented thinner compact bone (80.69 µm); however, in the CAF2 and CAF1 groups, this decrease in thickness was prevented by the consumption of Agave fructans (104.32 µm and 116.71 µm, respectively) (Fig. 6). The compact bone thickness values of CAF2 and CAF1 were very similar to the SHAM control (121.09 µm).

More about bone

Open Document