Animal Experiment: Male Wistar Rats Essay

Animal Experiment: Male Wistar Rats Essay

Length: 1081 words (3.1 double-spaced pages)

Rating: Strong Essays

Open Document

Essay Preview

MATERIALS AND METHODS
Animals
4-6 weeks old male Wistar rats weighing 80-120 g at study initiation, were obtained/purchased from the Animal House of Ahvaz Jondishapour University of Medical Sciences (AJUMS), Ahvaz, Iran. The rats were housed in an animal room maintained at 23 ± 3 °C and a relative humidity of 30-70 % with the altering light/dark cycle of 12h during the experiment and for at least, one week prior to sensitization period (for acclimatization purposes). The animals were housed in stainless-steel wire cages in groups of 5 and had free access to PN-free standard laboratory rodent chow and water.
All protocols involving the examined animals, were orchestrated in conformity with the instructions for Laboratory Animal Experiments in AJUMS Animal Research & Care Center.

Reagents
The reagents used in this research, were purchased from the sources enclosed in parentheses:
Alum [Al(OH)3] (Alhydrogel 2.0%, Serva Chemical Co., U.S.A), Cholera toxin (C-3012, Sigma Chemical Co., St. Louis, Mo, U.S.A), DMSO (Merck Chemical Co., Germany), Evan's blue dye (Merck Chemical Co., Germany), Rat histamine kit (LDN Chemical Co., Germany), K3-EDTA (Sigma Chemical Co., St. Louis, Mo, U.S.A), PBS (Merck Chemical Co., Germany), Rat Total IgE kit (ICL Chemical Co, U.S.A).
Unshelled/incrusted crude PNs were supplied from Safee-Abad Tree-plantar Research Station in the town of Dezfool, Khuzestan Province, Iran.

Antigen/Allergen Preparation
In the present study, PN proteins were used as allergens which were extracted from fresh crude PNs as follows, briefly:
PN bodies were pulverized by a mill and the resulted paste was defatted by n-Hexane (1:3 v/v, 3 times). Subsequent to separation process, residues were dried out and deodorized via gentle he...


... middle of paper ...


...t response was defined as a wheal reaction showing up as a blue area/circle measuring greater than 5 mm in diameter when read at 20 min post id PN-challenge.

Detection of Vascular Leakage
Five min before intra-peritoneal PN challenge-dose adminstration, 7 rats from each group received 200 μL of Evan's blue dye (5 mg/mL PBS) by tail vein injection. Footpads (& paws) of mice were examined for signs of vascular leakage (visible blue color) 40-45 min after ip administering of 200 μg of the filter-sterilized CPE.

STATISTICAL ANALYSIS
Data were analyzed using the SigmaStat statistical software package (SPSS, Chicago, Ill, Version 15). Statistical differences between two groups were analyzed by 1-way ANOVA. A probability value of less than 0.05 was considered statistically, significant.



Works Cited

Adjuvant, Anaphylactic Parameters, Peanut Allergy, Wistar-strain Model

Need Writing Help?

Get feedback on grammar, clarity, concision and logic instantly.

Check your paper »

Recriuiting the Most Suitable Laboratory Animals for Food Allergy Modeling

- During the last few years, most of the societies, all over the world, have been facing a progressive increase of adverse reactions to food. Accordingly, as many as 20-30% of the general population, in the gross, report some two adverse reactions to food components, of which, for the most part, allergic disorders are of considerable concerns owing to their epidemic increase in prevalence and morbidity. Our understanding of these diseases has enabled the search of therapeutic approaches that can either modulate the sensitization process or impact on allergic mediators and hereby, helping manage allergic symptoms/signs....   [tags: wistar model of peanut allergy]

Strong Essays
2176 words (6.2 pages)

Essay about Effects of Ability on Depression

- Does Administering Abilify Decrease Depression in Rats. Depression, for the purpose of this paper, is defined as the lethargy and immobility a rat experiences when put into a tank full of water during a forced swim test (Wee, Wang, Woolverton, Pulvirenti, & Koob, 2007). It is measured by the inability of the rat to swim, climb or keep their heads above the water over a short span of time (Arunrut, Alhandre, Chen, Cha, & Russo-Neustadt, 2009). Typically, in a forced swim test, the rat will swim hard for approximately 10 minutes, then give up and float until the experimenters take them out....   [tags: animal testing, rats, saline]

Strong Essays
1896 words (5.4 pages)

Experiment On Non Human Animal Essay

- Experiment On non-Human Animal Laboratory experimentation of animals has been a hot topic of debate for decades. Advocates of animal’s rights think that it can never be justified because it brings sufferings to animals. They equate animal experimentation to torture. On the other hand there are those who think that humanity should have no qualms about using nature for their own ends. There are more shades of opinion in between and they are frequently reflected in debates over this emotionally and ethically charged topic....   [tags: Animal testing, Animal rights]

Strong Essays
1152 words (3.3 pages)

The Human Cost Of Animal Experiments Essay

- In animal testing, there is a questionable accuracy of these tests. “The Human Cost of Animal Experiments” written by Katrina Fox talks about how some of these tests are not always correct or accurate. About two million Americans become ill from these drugs that were deemed good to release to the public. Out of those two million people, one hundred thousand people die from these drugs being released. The U.S. Food and Drug Administration approved two-hundred nine new drugs from 1976 to 1985 and those drugs caused severe side-effects....   [tags: Animal testing, Animal rights]

Strong Essays
1273 words (3.6 pages)

An Experiment On Animal Acts Essay

- ... Jealously is classified into the secondary category, which is not considered a simple emotion. This precise discovery in research has lead scientists and researchers to believe that dogs do in fact get jealous when not rewarded for doing the same task as another dog. While more data is still being collected and analyzed, the breakthrough contributes to the credibility to the research and development on animal cognition. Inclusively, from the research and data collected, there is enough evidence to support that dogs consistently do feel a sense of envy....   [tags: Emotion, Dog, Feeling, Experiment]

Strong Essays
1533 words (4.4 pages)

Essay about Animal Testing Should Not Be Treated

- It is estimated that each year, over 100 million animals-including mammals, birds, fish, and amphibians-are killed in U.S. laboratories for various testings, according to PETA.org. Americans should reconsider their usage of animal testing due to the fact that humans have an improper advantage to animals. Organizations such as PETA, do whatever possible to protect the rights animals have. Due to such organizations, there have been more reasons to find ways to no longer find the need to test on animals....   [tags: Animal testing, Experiment, The Animals]

Strong Essays
1135 words (3.2 pages)

Essay on Stop Animal Cruelty in Science Experiments

- Animals are not only bred inside laboratories, but also obtained from special dealers, animal shelters, and directly from the wild. Dogs and cats are usually obtained from Class A or B dealers. Class A dealers specialize in selling purpose-bred animals, or animals bred specifically for experimentation. Class B dealers buy animals at auctions, through newspaper ads, or animal shelters, and then sell them to laboratories. Animals acquired from shelters is known as “pound seizure”.1 In the United States, there are no federal laws prohibiting pound seizure, but Washington D.C....   [tags: Animal Testing, Product Tests]

Strong Essays
959 words (2.7 pages)

Essay on Animal Testing : Is It Ethically Sound?

- Animal Testing: Is It Ethically Sound. Throughout decades of scientific study, experimentation on animals has been the most common method of testing a product. Animals have been the innocent victims of harmful experiments for research, medicine, and cosmetics. While using animals to conduct experiments has been the most prefered procedure, it is not the most accurate. While primates such as chimpanzees share 99.9% similarities to humans, and rats are 98% genetically similar, they are not the same (Hajar)....   [tags: Animal testing, Animal rights, Experiment]

Strong Essays
1304 words (3.7 pages)

The Ethics Of Animal Testing Essay example

- Animals have held an important spot in many of our lives. Some people look at animals as companions and others see them as a means of experimental research and medical advancement. With the interest to gain knowledge, physicians have dissected animals. The ethics of animal testing have always been questioned because humans do not want to think of animals on the same level as humans. Incapable of our thinking and unable to speak, animals do not deserve to be tested on by products and be conducted in experiments for our scientific improvement....   [tags: Animal testing, Experiment, Animal rights]

Strong Essays
1280 words (3.7 pages)

Essay about Is Animal Experimenting (Rats, in Particular) Right or Wrong?

- When it comes the issue of killing a living being for a greater good, there are so many possible justifications for either side of the argument. If I were to take a look at this subject from a morality standpoint, I would have to approach this question differently for different types of living creatures. This could mean that the answer to the matter could change pending what creature is in question. An example of such an issue is the question to whether or not I can plausibly justify the practice of subjecting rats to these painful research experiments in a way that does not imply that it would also be permissible to subject human beings to the same painful experiments without their consent....   [tags: Research, Ethics, Babies]

Strong Essays
1117 words (3.2 pages)