A Brief Note On The Thin Layer Chromatography Essay examples

A Brief Note On The Thin Layer Chromatography Essay examples

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The Thin layer chromatography is one of the oldest techniques which is used to identify what is present in an unknown mixture. It is a very useful technique for research,forensics,environmental testing, and many more fields because it is simple and inexpensive way to analyze small samples. In this experiment two compounds (solid) were used ferrocene and acetylferrocene. In the first step 50 ml of 2:8 diethyl ether/petroleum ether was placed in an erlenmeyer flask covered with a parafilm paper to avoid evaporation. Then the glass column was prepared placing a small cotton on the bottom of the glass column and then added a small amount of sand , wich will give the stationary phase an even base and prevent concentration and streaking of the bands as they come off the column. We weighed 6.0 g of silica (white powder) and was placed on a flask, then it was mixed with the solvent (2:8 diethyl ether/petroleum ether to make our slurry, and it was drained through the column. Later we weighed 0.150g of 1:1 ferrocene and acetylferrocene(orange) mixture and we added silica, we mixed it togeth...

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