Wait a second!
More handpicked essays just for you.
More handpicked essays just for you.
what is the effect of PH on enzymes activities
discussion about enzymes
effect of temperature on enzyme activity
Don’t take our word for it - see why 10 million students trust us with their essay needs.
Recommended: what is the effect of PH on enzymes activities
An Investigation Into The Rate of Reaction of The Catalase Enzyme
Introduction:
In this experiment I will attempt to investigate the factors that
effect the rate of reaction of the catalyse enzyme.
Catalyse is an enzyme found in food such as potato and liver. It is
used for removing hydrogen peroxide because of the shape of the
hydrogen peroxide molecule; this type of reaction is when a molecule
Is broken down into smaller pieces called catabolic reaction.
Enzymes are proteins e.g. catalyse. They are made in cells. A catalyst
is something that speeds up a reaction.
One molecule can usually be used many times.
All enzymes work at different pHs because they have different jobs to
do but you cant use a ph which isn't suitable for that particular
enzyme which you are using, other wise the enzyme will de nature.
Enzymes also react with tempreture but like the ph the temperature
cant be unsuitable to the enzyme other wise it will de nature or slow
down the enzymes which would make it very difficult to obtain accurate
and reliable results.
A carbohydrase enzyme is different to a catalyse enzyme and other
enzymes because enzymes have different patterns (shape). Enzymes are
like keys to different substrates like e.g. fat, sugar and the
substrates are like keyholes.
Mass of potato
Volume
Of
Oxygen
Average
1g
1.8ml
1.8ml
1.6ml
2ml
2g
2.6ml
1.10ml
2.5ml
2ml
3g
4.2ml
3.6ml
3.8ml
4ml
4g
4.9ml
4.9ml
4.9ml
5ml
5g
5.5ml
5.1ml
5.6ml
5ml
6g
5.7ml
6ml
6ml
6ml
As you see from the preliminary results there where a lot of problems
along the way but this is only to help me set out the proper results
Catecholase is an enzyme formed by catechol and oxygen used to interlock oxygen at relative settings, and it is present in plants and crustaceans (Sanyal et. al, 2014). For example, in most fruits and vegetables, the bruised or exposed area of the pant becomes brown due to the reaction of catechol becoming oxidized and oxygen becoming reduced by gaining hydrogen to form water, which then creates a chain that is is the structural backbone of dark melanoid pigments (Helms et al., 1998). However, not all fruits and plants darken at the same rate. This leads to question the enzymatic strength of catecholase and how nearby surroundings affect its activity. The catecholase enzyme has an optimal temperature of approximately 40°C (Helms et al., 1998). Anything above that level would denature the tertiary or primary structure of the protein and cause it to be inoperable. At low temperatures, enzymes have a slower catalyzing rate. Enzymes also function under optimal pH level or else they will also denature, so an average quantity of ions, not too high or low, present within a solution could determine the efficiency of an enzyme (Helms et al., 1998). Also, if more enzymes were added to the concentration, the solution would have a more active sites available for substrates and allow the reaction rate to increase if excess substrate is present (Helms et al., 1998). However, if more
Investigation of How the Concentration of Catalase Enzyme Affects the Rate of Reaction Aim: To find out how the concentration of Catalase Enzyme will affect the enzyme activity and the rate of reaction towards Hydrogen Peroxide. (H O ) Prediction: I predict that with the higher concentration of enzyme, the likelihood of it breaking down molecules will be greater because there will be more enzymes to work at the substrate and the chances of it colliding will be higher making the activity time quicker. Equipment: · Syringe · Measuring Cylinder (×2) · Knife · Blender · Beakers (×2) · Balance · Hydrochloric Acid in a beaker · Stop clock · Potato · Water in a beaker Preliminary Experiment: In this experiment we will be using an enzyme called Catalase. By using different amounts of this enzyme we will be diluting it with water to test how the concentration of Catalase affects the rate of reaction with Hydrogen Peroxide.
The rate of reaction of Succinate dehydrogenase. Introduction: Enzymes are protein molecules that function as biological catalysts that can help break larger molecules into smaller molecules while remaining unchanged. They speed up the chemical reactions by lowering the energy of the activation barrier, specific to one molecule. The enzyme’s specificity arises from its active site, an area with a shape corresponding to the molecule with which it reacts (the substrate).
enzyme. As I'm sure you are well aware, enzymes do not get used up in
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
Investigating the Activity of an Enzyme Sucrose using the enzyme sucrase (invertase) can be broken down into. Glucose and Fructose -. The aim of this experiment was to find out about the activity of enzymes through measuring the micromoles of sucrase. expressed whilst the following reaction occurs:-. SUCRASE [ IMAGE ] SUCROSE GLUCOSE + FRUCTOSE C12H2201 H20 C6H12O6 C6H12O6
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
Investigating Factors that Affect the Rate of Catalase Action Investigation into the factors which affect the rate of catalase action. Planning Aim: To investigate the affect of concentration of the enzyme catalase on the decomposition reaction of hydrogen peroxide. The enzyme: Catalase is an enzyme found within the cells of many different plants and animals. In this case, it is found in celery.
The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.
Investigating the Rate of Reaction between Amylase and Starch. Plan Aim: To be able to The aim of this investigation is to find out whether the volume of amylase affects the rate of reaction between amylase and starch. Prediction: I predict that the greater the volume of amylase then the faster the rate of reaction between the starch and amylase. I predict this because of the lock and key hypothesis.
Investigating a Factor that Affects Enzyme Activity Planning -------- Aim --- To investigate a factor which will affect the activity of catalase, whilst keeping all variables constant. Possible Independent Variables ------------------------------ Here are a number of possible independent variables that could be changed in the experiment: Independent variable Continuous/Discontinuous Easy to measure?
The Effect of pH on Enzyme Activity. pH is a measure of the concentration of hydrogen ions in a solution. The higher the hydrogen ion concentration, the lower the pH. Most enzymes function efficiently over a narrow pH range. A change in pH above or below this range reduces the rate of enzyme reaction. considerably.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells
Investigating the Effect of the Enzyme Catalyse On Hydrogen Peroxide Introduction The aim of this experiment is to determine the effects of varying enzyme (catalyse) on Hydrogen Peroxide. Hydrogen Peroxide + Catalyse à Water + Oxygen 2H2O2 à H2O + O2 + Heat Apparatus & Diagram [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE] Bung Potato Hydrogen Peroxide Water Collected Oxygen Delivery Tube Measuring Cylinder [IMAGE] Using the Equipment Safely It is important that we use the apparatus carefully, as safety will be an issue throughout the whole experiment. We will wear goggles and an apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful, ensuring they don't come into contact with our eyes, skin or clothes. Catalyse is an enzyme found in all living cells.
If I was to do this experiment again I might use a Fungi amylase to