Temperture's Effect on Beet Membranes
Aim:
To investigate the effect of temperature on membrane structure
Hypothesis: The higher the temperature, the greater the amount of
pigment that leaves the beetroot cell.
1. An increase in temperature increases the kinetic energy of the
molecules within the cell, raising the rate of diffusion of
pigment out of the cell across the differentially permeable cell
membrane.
2. A further increase in temperature will cause the protein
molecules in the cell membrane to change shape, i.e. becoming
denatured. As a result, the red pigment in the vacuole will,
together with the cytoplasm, leak out through the damaged
membrane. Colour leakage will hence increase more significantly.
Apparatus and procedure
Alterations to the procedure
To wash away excess dye, the newly cut beetroot slices are not left
overnight but 2 minutes only, in order to save time.
Identifying variables:
Independent Variable
Dependant Variable
Controlled Variable
Temperature
Colour absorbency
Beetroot
Diameter and length of beetroot slices
Volume of water in each boiling tube
Time for washing away excess dye
Time for each boiling tube of water to equilibrate in each water bath
Time each boiling tube of water and beetoot was left in each water
bath
Safety precautions
1. Take care using the knife and cork borer.
2. Wear safety goggles in case splashes of red pigment enter your
eye.
3. Handle hot boiling tubes carefully.
Results
Temperature/°C
Colour Absorbency/A
0
0.03
10
0.02
30
0.06
40
0.23
50
0.92
60
1.85
70
1.76
A graph of colour absorbency against temperature is plotted to
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