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Investigating the Effects of Changing Concentration of Starch on the Digestion of Amylase

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Investigating the Effects of Changing Concentration of Starch on the Digestion of Amylase



Aim
===

To find out the effects of changing the concentration of starch on the
digestion of amylase



Introduction
============

In the digestive system starch is broken down into glucose and other
simple sugars by biological enzymes. Enzymes such as amylase, protease
and lipase act as catalyst to break up big molecules into small
molecules so the can pass through the gut wall. Amylase is produced in
three places - the stomach, pancreas and small intestine. In this
experiment we will see how long it takes for amylase to convert starch
into glucose. We will do this by using drops of iodine solution. If
the starch solution stays black starch is still present, if it turns
orange than starch is not present.



Plan
====

- In this experiment I will be using 5 different concentrations of
starch

- I will place 2 drops of iodine solution into a dimple tray

- Then I will take 5cm³ of amylase solution and put it into a test
tube

- To this I will add 5cm³ of starch solution

- Using a pipette I will immediately add 2-3 of the mixed solution
onto the iodine in the dimple tray and start the stop clock

- After every 30 seconds I will stop the stop clock, note down any
colour change and start it again

- I will do this for 240 seconds



Safety
======

To ensure this experiment is done in a safe way, the following points
should be considered: -

- Work surfaces should be cleared

- All chemicals and solution should be treated with care and not
swallowed

- Test tubes should be placed in test tube racks

- Safety goggle should be worn



Fair test
=========

Independent variable

- Concentration of starch

Dependant variable

- Time taken for effect

Control variables

- Same amount of amylase and starch solution

- Same amount of mixed iodine an starch solution out on the iodine for
each concentration

- Same time measured

- Same time colour change noted (every 30 sec)

- Same temperature

- Same enzyme (amylase) concentration



Prediction
==========

I predict that the higher the concentration of starch the faster it
will change colour. This is because if concentration of starch is
higher, it contains stronger starch and so the amylase has more starch
to convert



Apparatus
=========

- Starch solution

- Amylase

- Iodine solution

- Measuring cylinders

- Test tubes

- Dimple trays



Method
======

- Place 2 drops of iodine solution into the dimple tray

- Take 5cm³ of amylase solution into a test tube

- To this add 5cm³ of starch solution

- Immediately using a pipette add 2-3 drops onto the iodine solution
in the dimple tray and start the stop clock

- Record the results after every 30 seconds and note down any colour
change



Diagram
=======

Iodine solution

[IMAGE]

Mixed amylase and starch solution

[IMAGE][IMAGE]

1%

2%

3%

4%

5%



Results
=======

Starch concentration

Time (seconds)

0

30

60

90

120

150

180

210

240

1%

Black

Black

Black

Black

Black

Black

Black

Brown

Brown

2%

Black

Black

Black

Black

Black

Black

Brown

Brown

Orange

3%

Black

Black

Black

Black

Black

Brown

Orange

Orange

Orange

4%

Black

Black

Black

Black

Brown

Orange

Orange

Orange

Orange

5%

Black

Black

Black

Brown

Brown

Orange

Orange

Orange

Orange

Conclusion

By doing this experiment I have proved my prediction to be correct and
that the higher concentration the less time it took for the starch to
digest. This is because the starch was stronger and purer and so the
amylase was able to act on it straight away easily digest it. The
weaker concentration of starch took longer to digest because it was
less concentrated and so the amylase took longer to digest it, as the
starch wasn't strong enough

This experiment has helped me learn the way amylase breaks starch down
into smaller glucose molecules and what factors can affect this
process.



Evaluation
==========

Overall I think this experiment went well. The results turned out a
expected making them quite accurate and reliable, but I think it would
have been better if we repeated the whole experiment at least 3 times
to account for any anomalous results and to gain a wider set of
results.

I think the method was quite good but the temperature was not kept
constant. This could have affected the results. If I repeat this
experiment I will put the test tubes in a water bath and monitor the
temperature regularly. The water will help keep the temperature the
same throughout the experiment making it a fairer test.

How to Cite this Page

MLA Citation:
"Investigating the Effects of Changing Concentration of Starch on the Digestion of Amylase." 123HelpMe.com. 24 Apr 2014
    <http://www.123HelpMe.com/view.asp?id=121479>.




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