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Investigating the Effect of Sucrose Solution on Osmosis in Potato Tissue

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Investigating the Effect of Sucrose Solution on Osmosis in Potato Tissue

To investigate the effect of concentration of sucrose solution on
osmosis in potato tissue

HYPOTHESIS

The prediction for this experiment: when the potato tissue is put in
concentrated sucrose solution, the vacuole and the cytoplasm of potato
cell will shrink and become flaccid .A potato cell is said to be
flaccid when the potato cell losses it's firmness and begin to be
wilt. This is because the concentration of the sucrose solution is
more than the concentration of the cytoplasm.

THEORY

Osmosis is the diffusion of water molecule of water through a
partially permeable cell membrane from a high concentration of water
to a low concentration of water until the concentration is equal.

Osmosis occurs in all plant and animals ell. It is useful in many
ways. For example, our body absorbs water from fluid by osmosis. The
kidney retiree water and blood, this is the process of osmosis. The
plant roots absorbs water form the soil by osmosis.

When a potato chip is put diluted water, it takes in water by osmosis
through the partially permeable cell membrane; after some time the
potato cell will swell and become heavier and bigger in size. The
potato cell will not burst because the cell membrane is surround by a
cell wall. This cell will become turgid. However, when a potato cell
is put in a concentrated sucrose solution, the cell will shrink. In
addition, the cell will become flaccid this means that the cell has
lost the firmness and begin to wilt. This is because the concentration
of sucrose solution is more than the concentration of the cytoplasm

A DIAGRAM OF A PLANT CELL

[IMAGE]


When potato tissue is put in diluted concentrated sucrose solution for
exeample.0.4M of concentrated sucrose solution (8cm3 of sucrose
solution and 12cm3 diluted water). The water will move through both
direction of the potato's membrane, but will move in one direction
than the other direction. When the concentration of the sucrose is the
same on both side of the membrane .At this point the net movement of
water is zero, and zero is the equilibrium of the net movement of
water.

[IMAGE]

[IMAGE]

[IMAGE]




EXPECTED RESULT
---------------

My result is based on my hypothesis and pilot study. My result graph
looks like downward slope because when the concentration of sucrose
solution increase the mass of the potato decreases meaning the more
the concentration of sucrose solution the more the potato cell
shrinks.


[IMAGE]

Concentration of sucrose solution (M)

1

0.9

0.8

0.7

0.6

0.5

0.4

0.3

0.2

0.1

0

Mass before (g)

1.7

1.7

1.7

1.7

1.7

1.7

1.7

1.7

1.7

1.7

1.7

Mass after (g)

0.4

0.5

0.88

1.09

1.33

1.51

1.63

1.74

1.79

1.94

2.01

Change in mass (g)

-1.3

-1.2

-0.84

-0.61

-0.37

-0.19

-0.17

+0.04

+0.09

+0.24

+0.3

Change in percentage (%)

-76

-70.5

-48

-35.9

-21.8

-11.2

-4.1

+2.3

+5.3

+14.1

+18.2

Variables:

Independent variable: The independent variable is also known as the
output variable. In this experiment, the input variable of this
experiment is the concentration of the sucrose solution.

Dependent variable: The dependent variable in this experiment is the
mass of the potatoes

Other variable: the other variables are variable that could stop you
from having a wrong result in this experiment. This could wrong
measurement when diluting the concentrated sucrose solution.
Inaccurate mass of the potatoes

Apparatus:

* 33 plastics breaker

* Core borer

* 2 potatoes

* Measuring cylinder (big and small size)

* String

* Paper towel

* Slotting title

* Digital beam balance

* Label

* Stop clock

* 330 cm3 of concentrated sucrose solution in a large beaker

* 330cm3distilled water in a lager beaker

PROCEDURE:

1. Collect all the apparatus you need.

2. Label the beakers accordingly

3. Dillute the sucrose solution using the table below

Concentration of sucrose solution (M)

1

0.9

0.8

0.7

0.

0.5

0.4

0.3

0.2

0.1

0

Volume of sucrose solution required (cm3)

20

18

16

14

12

10

8

6

4

2

0

Volume of distilled water required (cm3)

0

2

4

6

8

10

12

14

16

18

20

4. Each concentration must be repeated in three breakers

5. Bore the potatoes on a slotting title.

6. Weight the potatoes and record their mass.

7. Put the pieces of potatoes in the beakers and to note of the
concentration and the mass of potatoes in it.

8. After some hours. Slightly dry the potatoes with paper towel then
weight the mass of the potatoes.

9. Make a record table of your result.

Measurement:

When diluting the concentrated sucrose solution I use big and small
measuring cylinders when needed to get an accurate measurement. The
concentrated sucrose solution is measurement in mole. The range
measurement is from 0 -1 mole. The potato pieces have a different
measurement from each other.

Fairness Precaution:

The fair precautions in this help to give an accurate result
experiment are: Allowing the potato tissue stay longer in the
concentrated sucrose solution to allow the solution pentrate fully to
the core of the sample. By repeating, the test for each concentration
and this will decrease errors. Being more accurate when diluting the
sucrose solution with the approve amount of distilled water will also
reduces errors. Making sure the potatoes are recorded accurately and
the concentration of sucrose where the potato was put in was recorded.

Safety: I will ensure safety around the lab by: wearing safety
goggles, lying paper towel when drying the potatoes, removing baggage
from walking space. Push stool under the table, walk carefully and
slowly and handle apparatus very carefully.

Data Recording:

Concentration of sucrose solution (M)

1

0.9

0.8

0.7

0.6

0.5

0.4

0.3

0.2

01.

0

Mass [1]

Before [2]

(g) [3]

Average

Mass [1]

After [2]

(g) [3]

Average

Change in mass (g)

Percentage change (%)

Data presentation:

Concentration of sucrose solution (M)

1

0.9

0.8

0.7

0.6

0.5

0.4

0.3

0.2

0.1

0

Mass [1]

0.72

0.75

0.88

0.55

0.60

1.08

0.92

1.40

0.98

1.29

1.21

before [2]

0.73

0.88

0.89

0.78

0.83

0.79

1.00

1.21

0.70

0.82

1,49

(g) [3]

0.83

1.16

0.98

0.82

0.72

1.02

1.21

1.48

0.81

1.12

1.50

Average

0.76

0.93

0.91

1.72

0.72

0.96

1.04

1.36

0.83

1.8

1.4

Mass [1]

0.4

0.5

0.6

1.2

0.8

0.9

0.8

1.5

1.0

1.3

1.4

after [2]

0.6

0.9

0.6

0.6

0.6

0.7

0.9

1.3

0.5

1.0

1.7

(g) [3]

0.7

0.6

0.7

1.2

0.7

1.1

1.2

1.4

0.6

1.2

1.8

Average (g)

0.57

0.6

0.63

0.83

0.7

0.9

0.97

1.4

0.7

1.17

1.6

Change in mass (g)

-0.19

-0.33

-0.28

-0.89

-0.02

-0.06

-0.07

0.04

0.05

0.2

Percentage change (%)

[IMAGE]

result:

EVALUATION: Although my result supports my hypothesis. There were
several anomalous results and a large deviation for each result; This
means there were some reasons that made they quit different from each
other, which are: By allowing the potato to stay longer in the
concentrated sucrose solution to give you more accurate result. Also
using small and big measuring cylinder to obtain an accurate
measurements when diluting the concentrate sucrose solution, I was
also more accurate in diluting the concentrated sucrose solution. The
concentration was also repeated several times unlike in my pilot
study.

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"Investigating the Effect of Sucrose Solution on Osmosis in Potato Tissue." 123HelpMe.com. 18 Apr 2014
    <http://www.123HelpMe.com/view.asp?id=120969>.




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