Currently a period of rapid chromatography heyday is undergoing, its scope is extremely wide and includes sectors such as biotechnology, medicine, forensics, organic synthesis, environmental monitoring, obtaining ultrapure substances, analysis of space objects and more. A special place in the list of chromatographic methods takes liquid chromatography - the most versatile method of analysis based on non-destructive separation of substances. The latter allows applying liquid chromatography not only as an analytical, but also as a unique technological method for the isolation and purification of substances, when other methods are ineffective. One should also not forget that the very discovery and subsequent development of chromatographic methods started with liquid chromatography. There is a separate branch of liquid chromatography dubbed biomedical HPLC attached to biology and medicine. Objects of its research are the analysis of endogenous substances and drugs in biological fluids (blood, urine, saliva). Most of the researches in clinical biochemistry and clinical pharmacokinetics are successfully implemented using biomedical HPLC.
Amino acid analysis in biological fluids, primarily homocysteine, appears to be very important clinical problem. Hyperhomocysteinemia increases the risk of early development of atherosclerosis, thrombosis, coronary and cerebral blood vessels, and is a predictor of death (Barker, n.d.). Existing analytical techniques are complex and costly, both in time and in finance. So, providing a simple and reliable technology is required. For example, thiols, including homocysteine, cysteine and glutathione, are transparent to UV rays, and are not captured by the chromatographic apparatus. This, incidentally, is tr...
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The complete experimental procedure is available in the General Chemistry Laboratory Manual for CSU Bakersfield, CHEM 213, pages 20-22, 24-25. Experimental data are recorded on the attached data pages.
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...s the change in the temperature of both of these batches, 6°C for the pure, and 13°C for the crude. In this final sub-section of the Characterization of Aspirin, the values of absorbance were recorded. Initially, 0.0566 grams and 0.0590 grams of pure and crude Aspirin respectively were obtained and each individually placed into beakers (400 milliliter) and had 250.0 milliliters of distilled water added to them. From each beaker, a tiny amount of the just dissolved solutions was transferred to a cuvette, one cuvette for each type of aspirin. Each cuvette was placed into the ultraviolent spectroscopy mechanism which was connected to a computer and absorbance spectrum values were obtained at 298 nm (Figure 5) (0.1987 pure aspirin, and 0.9549 crude aspirin).
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We thank the University of Oklahoma and the chemistry faculty for providing the space, instructions, and equipment for the development of this report and experiment.
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...efore, is to detect and determine phenolic acids in pig blood by examining levels in red blood cells, plasma, albumin, low density lipoprotein (LDL),and very low density lipoprotein (VLDL). It is important to note that when a phenolic compound is present in body fluids, it generally occurs in one or several conjugated forms such as methyl, glucuronide and sulfate, (Proudfoot, Puddey, Beilin, Stocker, & Croft, 1997). There are several methods used to determine bioavailability of phenolic acids and possible metabolites in blood. Some of these methods include electron impact mass spectrometer (EI-MS), atmospheric pressure chemical ionization mass spectrometer (APCI-MS), electro-spray ionization mass spectrometer (ESI-MS), and matrix-assisted laser desorption/ionization mass spectrometer, (S Bertuglia, et al) as well as the more conventional HPLC after identification.
Due to the nature of amino acids, a titration curve can be employed to identify
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