The Benedict's Test is used to test the presence of simple sugars in a sample. If sugars are present, a color change will occur from blue to red. However, although the Benedict's test shows the presence of sugars, it cannot accurately determine the concentration of sugar in a sample solution. In our method, we added specific concentrations of glucose to the Benedict's test to use as a chart to estimate the glucose concentration of an unknown solution X. Although this gives a rough estimate of the
that product can be used to measure the reaction rate using a spectrometer (Schultz, 2006). We started with twice the volume of the stock, so for the case of Part 1 was to begin with 6 ml of full strength enzyme. Two test tubes were used for the dilution. The first contained 5ml of buffered catechol and the second with 5ml of distilled
having similar cells. There are many methods by which pure cultures can be obtained a few are discussed below. SERIAL DILUTION. Serial dilution is a technique in which we find out the overall count of colonies present in the given sample. Serial dilution is followed by spread plate technique or the pour plate technique for the further isolation of microbes. PROCEDURE FOR SERIAL DILUTION. This procedure is applicable for finding out the total number of colonies from water and soil samples. The procedure
The objective of this study was to record a quantitative approximation of how many bacteria are present within various samples of meat products. The bacterial content of each meat sample is vital information in regards to improvements within the meat processing industry, and gives reason for changing or sustaining current feeding and processing conditions. Understanding which methods taken in processing meat that is sold to the public is a matter of public health, as obtaining and maintaining lower
into 25 main squares, each of which is subdivided into 16 smaller squares. The volume of suspension above the smallest squares is a uniform 0.00025 mm³. So the the number of cells in the culture suspension is given by: D*N S*C Where D= dilution of original culture N= total number of cells counted S= number of squares counted C= volume of 1square The experiment is taken over 5 days, and every 24 hours a reading is taken. Raw results will be tabulated first, with headings of
Dilution is a process of reducing the concentration of a chemicals in a solution. The dilution is done by adding more solvent to the solution. After that, this solution is mixed thoroughly to get the uniform concentration. A unit volume of a liquid material of interest combined with an appropriate volume of a solvent liquid in order to achieve desired concentration is called simple dilution. The dilution factor is the total number of unit volumes in which material will be dissolved. The diluted material
Salt Dilution Method Salt dilution method is used in mountain rivers with rough cross section, big boulders and turbulent flow. An electrical conductivity (EC) meter was used for discharge measurement. Salt dilution method is particularly used for measurement of flow in mountainous stream and any other sites where high degree of turbulence exists to ensure complete mixing of injected solution in the pre-determined length of the channel. Under such condition of flow, the conventional approach to
calibration solution 3 D. Aliquot versus serial dilution method 9 Discussion 11 References : 11 List of Tables and figures: Table 1 Data for Aliquot method 2 Table 2 Data for Serial dilution method 2 Table 3 Concentration Data for Aliquot method 4 Table 4 Experimental andf actual concentration for aliquot method 4 Table 5 Concentration data for serial dilution method 7 Table 6 Experimental and actual concentration for serial dilution method 7 figure 1 Aliquot dilution method …………………………………………………………………………………………………………
Figure 1 is an illustration of lab 1, the serial dilution performed for bacteria. This table is describing the number of colony forming units and the observations of the plates per dilution after a week. We didn’t stain the bacteria because time was limited and we would have only determined if they were gram positive or negative. Lab 2. After 72-hours of incubation at 37°C the fungi plates were refrigerated for a week. They were then observed, and a plate count was performed. A geometric mean
1. What is Martha Stewart the brand? What is the brand identity? Do multiple brand identities exist? Martha Stewart's brand stands for stylish and traditional American living. She is brilliant at channeling the taste and passion of her customers into new products and media. She is said to turn "dreamers into doers". Taking this in to the context of how corporate strategists want the brand to be perceived by internal and external constituents arrives at a brand identity. The core identity or
In the Matal v. Tam court case, the court settled certain aspects of the First Amendment law while it opened up new issues in trademark law. It is a challenge for the uninitiated to follow a coherent path through the court’s First Amendment. Tam and his band, The Slants, sought to register the band’s name with the U.S. Trademark Office. The Office denied the application because it found that the name would likely be disparaging towards “persons of Asian descent.” The office cited the Disparagement
Investigating the Effects of Dilution on the Rate of Reaction Between Sodium Thiosulphate and Dilute Hydrochloric Acid Aim: To investigate, the effects of dilution on the rate of reaction between sodium thiosulphate and dilute hydrochloric acid. Background information: The dilution variable does not occur to the hydrochloric acid but it occurs to the sodium thiosulphate. The reason why it does not occur in hydrochloric acid is because it needs to be constant and it is already dilute
produce a natural source of antioxidants (Alim-un-Nisa et al., 2012). For the watermelon juice, the storage time used is 0 hours, 4 hours, 8 hours and 12 hours. For the 0 and 4 hours storage time of watermelon juice, the dilution for plating is 10-0, 10-2, and 10-3. All of the dilution plates is duplicate to get more accurate result. The colony counting will be carried out after 24-48 hours. For watermelon juice with 0 hours storage time for both of the sample, it shows that many
the seeded water contain enough nutrient for the bacteria for 7 days. Conclusion After we conducted the experiment, we found out the sample with the least dilution factor has the biggest BOD value. We also found out BOD value is inversely proportional fraction of waste water volume and total combined volume. In conclusion, the biggest dilution factor, the more BOD value and the worse water quality. There are some errors in the experiments but most of them can be eliminated to produce a more accurate
reputation of trademarks is important and the requirement for a mark to be ‘well known’ is more than mere reputation. In this direction, ITC Case has well set the path for strict compliance of ingredients required under Sec. 29(4) for action against dilution of well known trademark.
placed on the bacterial plate at various dilutions, then the diameters of the dead bacteria would also be
a neutral pH. Urine is known to contain high levels of the 3 most used nutrients fior plant growth (NPK). Human urine is also known to contain other necessary nutrients. Too much nitrogen can burn the plant roots and cause spindly weak plants, so dilution of human urine is
a serial dilution to get the most accurate OD reading . The line of best fit equation for the standard curve is y=0.5394x ensuring to go through the point of origin. (See Table 1) Table 2 shows the product and the FDA Food Label with the mg/ml of each Dairy product . By this table it shows the Muscle Milk having the highest protein concentration (see Table 2) Before the 1:50 dilution, the 1:1 dilution shows the Whole Milk having the highest protein concentration. The Serial dilution is mentioned
enzyme concentration (graph 1), the increase of enzyme dilution results in a slower decrease of amylose percentage. Looking at the graph, the amylose percentage decreases at a fast rate with the undiluted enzyme. However, the enzyme dilution with a concentration of 1:3 decreased at a slow rate over time. Additionally, the higher the enzyme dilution, the higher the amylose percentage. For example, in the graph it can be seen that the enzyme dilution with a 1:9 concentration increased over time. However
SEA-PHAGES Fatma Sahin April 4th, 2018 Introduction: Bacteriophage is a phage where it plays in the role of being a virus which infects bacteria host and replicates within bacteria. Phages are not able to replicate on their own and due to this reason, they demand a bacterial host in order to reproduce. These viruses are only specific for their own host. The purpose of this lab is to be successful at amplifying phages presented in the environmental soil sample. The first soil sample