The micro-organism used in this lab was E.coli, which is a prokaryote. In general, prokaryotes are small, compact genomes that have a single, round DNA molecule. The genes are tightly packed, with almost no space between them. In the case of E.coli, it has thrA, 1 nucleotide, thrB, thr C.
Enzymes of a single biochemical pathway are coded by the operon: introns are absent, so all genes are not interrupted and the sequence is non-repetitive. An operon is a group of adjacent genes which are transcribed as a unit into a single polycistronic mRNA molecule. The lac operon is necessary for the transportation and metabolism of lactose.
An operon is made up of three features: promoter, operator and structural genes. The promoter site is where the RNA polymerase (Plac) is attached. The operator is the site where repressor protein binds, blocking RNA polymerase from binding, which in turn stops the transcription of the operon. Lastly, there are the structural genes, which are numerous genes, which code for the enzymes of a metabolic pathway, transcribing as a unit.
There are two techniques ...
The affects of pH, temperature, and salt concentration on the enzyme lactase were all expected to have an effect on enzymatic activity, compared to an untreated 25oC control. The reactions incubated at 37oC were hypothesized to increase the enzymatic activity, because it is normal human body temperature. This hypothesis was supported by the results. The reaction incubated to 60oC was expected to decrease the enzymatic activity, because it is much higher than normal body temperature, however this hypothesis was not supported. When incubated to 0oC, the reaction rate was hypothesized to decrease, and according to the results the hypothesis was supported. Both in low and high pH, the reaction rate was hypothesized to decrease, which was also supported by the results. Lastly, the reaction rate was hypothesized to decrease in a higher salt concentration, which was also supported by the results.
One of the most primitive actions known is the consumption of lactose, (milk), from the mother after birth. Mammals have an innate predisposition towards this consumption, as it is their main source of energy. Most mammals lose the ability to digest lactose shortly after their birth. The ability to digest lactose is determined by the presence of an enzyme called lactase, which is found in the lining of the small intestine. An enzyme is a small molecule or group of molecules that act as a catalyst (catalyst being defined as a molecule that binds to the original reactant and lowers the amount of energy needed to break apart the original molecule to obtain energy) in breaking apart the lactose molecule. In mammals, the lactase enzyme is present
E. coli produces very rapidly; a single microscopic cell can divide to form a visible colony with millions of cells overnight (phschool.com). It is the preferred bacteria in most laboratories because it grows fast and easy, and can obtain energy from a wide variety of sources. Since the birth of molecular cloning, E. coli has been used as a host for introduced DNA sequences (biotechlearn.org.nz). In 1973, Boyer and Cohen showed that two short pieces of DNA could be cut and pasted together, and returned to E.coli.
Mader, S. S. (2010). Metabolism: Energy and Enzymes. In K. G. Lyle-Ippolito, & A. T. Storfer (Ed.), Inquiry into life (13th ed., pp. 105-107). Princeton, N.J: McGraw Hill.
Proteins are one of the main building blocks of the body. They are required for the structure, function, and regulation of the body’s tissues and organs. Even smaller units create proteins; these are called amino acids. There are twenty different types of amino acids, and all twenty are configured in many different chains and sequences, producing differing protein structures and functions. An enzyme is a specialized protein that participates in chemical reactions where they serve as catalysts to speed up said reactions, or reduce the energy of activation, noted as Ea (Mader & Windelspecht).
Metabolic pathways are a series of reactions catalysed by multiple enzymes. Feedback inhibition, where the end product of the pathway inhibits an earlier step, is an
There are numerous types of bacteria that can be found in every environment. Each bacterium has different morphology which includes shape, texture and pigment production. These bacteria also have different food requirements which are important in being able to identify a microorganism. Microorganisms are a diverse group containing all bacteria a single cell prokaryotic organism that is found in every type of environment, archea single cell microorganism that lacks nuclei and almost all microorganisms are protozoa a unicellular eukaryotic organism. By identifying the causative agent of a bacterium within an individual, an antibiotic can be developed to prevent health issues. Microorganisms are also used to make certain food products for human consumption. An example of this would be the production of yogurt. It has probiotics that help with digestive abnormalities amongst other things. Probiotics are microorganisms that are consumed to provide health benefits in the body. Probiotics work by replacing the disturbed microbe with ones that are useful to digest. With the methods that wer...
Transcription factors are proteins that help turn specific genes "on" or "off" by binding to nearby DNA.
A Prokaryote is a single cell organisms that does not have a nucleus, which is divided into two major groups: archaeabacteria and bacteria. Prokaryotes are usually found in three common structures, such as bacillus is rod shaped, crocus is spherical-shaped, and spirillum is long shape. Although there are prokaryotes that are responsible for diseases there are also good types of bacteria that we need. Prokaryotes provide essential services to biological systems for instance food and agriculture.
Bacteria are single celled microbes. Bacteria reproduce by binary fission. In this process, the bacterium, which is a single cell, divides into two identical daughter cells. Binary
Madar, Sylvia S., & Windelspecht, Michael. (2014). Inquiry into Life, Metabolism: Energy & Enzymes (pp. 104-107). New York: McGraw Hill.
Secondly the gene has to be cut from its DNA chain. Controlling this process are many restriction endonucleases (restriction enzymes). Each of these enzymes cut DNA at a different base sequence called a recognition sequence. The recognition sequence is 6 base pairs long. The restriction enzymes PstI cuts DNA horizontally and vertically to produce sticky ends.
Enzymes are protein molecules that are made by organisms to catalyze reactions. Typically, enzymes speeds up the rate of the reaction within cells. Enzymes are primarily important to living organisms because it helps with metabolism and the digestive system. For example, enzymes can break larger molecules into smaller molecules to help the body absorb the smaller pieces faster. In addition, some enzyme molecules bind molecules together. However, the initial purpose of the enzyme is to speed up reactions for a certain reason because they are “highly selective catalysts” (Castro J. 2014). In other words, an enzyme is a catalyst, which is a substance that increases the rate of a reaction without undergoing changes. Moreover, enzymes work with
It is a single-celled organism that is not visible to the human eye, which means it can only be seen with a microscope. Bacteria are classified as Prokaryotes. They make their own food from the sunlight and can absorb food from the materials that they live on.
Prokaryotes include several kinds of microorganisms, such as bacteria and cyanobacteria. Eukaryotes include microorganisms as fungi, protozoa, and simple algae. Virus cells often consist of just a nucleic acid either DNA or RNA in a protein capsule. Viruses are considered neither prokaryotes nor eukaryotes because they lack the characteristics of living things, except the ability to replicate (which they accomplish only in living cells).