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Differences between eukaryotes and prokaryotes
Differences between eukaryotes and prokaryotes
Differences between eukaryotes and prokaryotes
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Introduction
In this lab, we observed different prokaryotic and eukaryotic cells, and then we observed a termite gut to see the different unicellular microorganisms that it contained. The purpose of this lab is to observe living and prepared bacterial cells, then describe their morphology, observe and describe the primary features of the eukaryotic cell, and to investigate and observe the organisms involved in a symbiotic relationship. By observing these things, we are hoping to find out what we should expect to see in yogurt bacteria. In order to predict this, we must first know the differences between prokaryotic and eukaryotic cells. Prokaryotic cells are organisms that do not have a cell nucleus, or any other membrane-bound organelles. Eukaryotes, on the other hand, does have membrane-bound organelles, most characteristically, a nucleus. Perhaps the most important difference between these two cells is that eukaryotes have a “true” nucleus housing their genetic material, whereas the DNA of a prokaryote is not membrane-bound. While many eukaryotes do not have a cell wall, the cell walls of prokaryotes are usually formed of a different molecule than those of eukaryotes. Eukaryotes are generally much larger than prokaryotic cells, however, prokaryotes have a larger surface area to volume ratio which gives them a faster metabolism rate, higher growth rate, and as a result, a shorter generation time than that of the eukaryotes. There are two major kinds of prokaryotes: bacteria and archaea (single-celled organisms). All prokaryotes share four main structures: the plasma membrane, cytoplasm, ribosomes, and genetic material. Some prokaryotic cells also have a cell wall, flagella, and pili. Bacterial cells have three different shapes:...
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...aterials needed for this part of the lab include: a microscope slide, 0.5% sodium chloride (NaCl) solution, forceps, a termite, a coverslip, and a microscope.
First, place a drop of 0.5% sodium chloride (NaCl) solution on a microscope slide. Then, use a pair of forceps to place a termite in the drop of saline solution. Quickly place a coverslip on top of the termite and gently press down on the coverslip. Observe the preparation under the scanning objective (4X) of the microscope and locate the abdominal region of the squashed termite. Then, observe the fluid around the squashed gut tissue under low and high power for the presence of flagellates and spirochete bacteria.
Works Cited
http://www.cod.edu/people/faculty/fancher/prokeuk.htm http://www.diffen.com/difference/Eukaryotic_Cell_vs_Prokaryotic_Cell http://www.shmoop.com/biology-cells/prokaryotic-cells.html
They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species.
Phenotypic methods of classifying microorganisms describe the diversity of bacterial species by naming and grouping organisms based on similarities. The differences between Bacteria, Archaea and Eukaryotes are basic. Bacteria can function and reproduce as single cells but often combine into multicellular colonies. Bacteria are also surrounded by a cell wall. Archaea differ from bacteria in their genetics and biochemistry. Their cell membranes are made with different material than bacteria. Just like bacteria, archaea are also single cell and are surrounded by a cell wall. Eukaryotes, unlike bacteria and archaea, contain a nucleus. And like bacteria and archaea, eukaryotes have a cell wall. The Gram stain is a system used to characterize bacteria based on the structural characteristics of their cell walls. A Gram-positive cell will stain purple if cell walls are thick and a Gram-negative cell wall appears pink. Most bacteria can be classified as belonging to one of four groups (Gram-positive cocci, Gram-positive bacilli, Gram-negative cocci, and Gram-negative bacilli) (Phenotypic analysis. (n.d.).
Its morphology consists of a rod-shaped gram negative bacteria that is commonly found in soil, water, vegetation, human intestines, as well as the intestines of animals. Its presence can be good or bad. The family Enterobacteriaceae consists of a large number of genera which are genetically and biochemically related to one another. Escherichia coli is a component of the family. This bacterium was discovered by a German pediatrician and bacteriologist named Theodor Escherich in 1885.
Eukaryotic Cells are Deemed as a Result of the Evolution of Symbiotic Prokaryotes Both Prokaryotic and Eukaryotic cells over time have sustained very dynamic changes from one another. More specifically we have seen the appearance of a more complicated and organized cell structure, the nucleus. However the big question amongst scientists today is how did these changes first occur? A fundamental concept of this evolution is the belief in the natural progression 'from the simple, to the more complex.' However one popular theory that argues that Prokaryotic symbiosis was responsible for forming the Eukaryotic nucleus is the 'Endosymbiotic Theory' this theory was first proposed by a former Boston University Biologist known as Lynn Margulis in the 1960's.
Eukaryotic cells, whether from animals, plants, protists, or fungi, are the most structurally advanced of the major cell types. Eukaryote are single-celled or multicellular organism whose cells contain nucleus and any other structures (organelles) enclosed within the membrane that perform specific functions. The surface of the cell is covered with a thin film or plasma membrane, which is the boundary that separates the living cell from its nonliving surroundings. Plasma membranes are composed mostly of proteins and lipids (Simon, 02/2012, p. 59-60).
Planning Firstly here is a list of equipment I used. Boiling tubes Weighing scales Knife Paper towels 100% solution 0% solution (distilled water) measuring beakers potato chips Cork borer. We planned to start our experiment by doing some preliminary work. We planned to set up our experiment in the following way.
Bacterial cells, like plant cells, are surrounded by a cell wall. However, bacterial cell walls are made up of polysaccharide chains linked to amino acids, while plant cell walls are made up of cellulose, which contains no amino acids. Many bacteria secrete a slimy capsule around the outside of the cell wall. The capsule provides additional protection for the cell. Many of the bacteria that cause diseases in animals are surrounded by a capsule. The capsule prevents the white blood cells and antibodies from destroying the invading bacterium. Inside the capsule and the cell wall is the cell membrane. In aerobic bacteria, the reactions of cellular respiration take place on fingerlike infoldings of the cell membrane. Ribosomes are scattered throughout the cytoplasm, and the DNA is generally found in the center of the cell. Many bacilli and spirilla have flagella, which are used for locomotion in water. A few types of bacteria that lack flagella move by gliding on a surface. However, the mechanism of this gliding motion is unknown. Most bacteria are aerobic, they require free oxygen to carry on cellular respiration. Some bacteria, called facultatibe anaerobes can live in either the presence or absence of free oxygen. They obtain energy either by aerobic respiration when oxygen is present or by fermentation when oxygen is absent. Still other bacteria cannot live in the presence of oxygen. These are called obligate anaerobes. Such bacteria obtain energy only fermentation. Through fermentation, different groups of bacteria produce a wide variety of organic compounds. Besides ethyl alcohol and lactic acid, bacterial fermentation can produce acetic acid, acetone, butyl alcohol, glycol, butyric acid, propionic acid, and methane, the main component of natural gas. Most bacteria are heterotrophic bacteria are either saprophytes or parasites. Saprophytes feed on the remains of dead plants and animals, and ordinarily do not cause disease. They release digestive enzymes onto the organic matter. The enzymes breakdown the large food molecules into smaller molecules, which are absorbed by the bacterial cells. Parasites live on or in living organisms, and may cause disease. A few types of bacteria are Autotrophic, they can synthesize the organic nutrients they require from inorganic substances. Autotrophic bacteria are either photosynthetic or Chemosynthetic. The photosynthetic bacteria contain chlorophyll that are different from the plant chlorophyll. In bacterial photosynthesis, hydrogen is obtained by the splitting of compounds other than water.
Prescott, Harley & Klein (1990) describe bacteria as prokaryotic cells (cells that lack a true membrane enclosed nucleus). Bacteria are both small and simple in structure; they usually are between o.5 and 5cmm yet they have many characteristic shapes and sizes. Some bacteria are circular or oval shaped, they are known as cocci bacteria. Other bacteria are rod-shaped, they are known as bacilli bacteria, and some bacteria are spiral and coil-shaped and it is know as spirilla bacteria.
solutions and add them to a test tube rack. I will then cut up a
Every cell, either prokaryotic or eukaryotic all contain basic cell parts. They are: a plasma membrane, cytoplasm, DNA (the genetic material), and ribosomes. Prokaryotic cells have a simple structure and they are usually smaller than eukaryotic cells. Also, most prokaryotic cells contain a cell wall. In addition to having the basic cell parts, eukaryotic cells also contain a membrane-bounded nucleus and cell organelles.
Prokaryotic cell: have no membrane covered organelles, they also have circular DNA and bacteria, Eukaryotic cell: have membrane covered organelles, they also have linear DNA and all other cells. Also the cell cycle is short in prokaryotic cells, roughly taking about 20-26 minutes to complete. And in eukaryotic cells, the cell cycle is long, it usually takes about 12-24 hours to complete. Below is a table of some of the differences between the cells:
We separated into groups of two each, making sure that we had the following materials for the lab: graduated cylinder, plastic sandwich bag, starch solution, twist tie, 500-mL beaker, iodine solution, and a pair of goggles and an apron (for the person handling the iodine solution).
Microbes are major key components in both are homes and industrial food preparation. There are number of lactic acid which is a form of bacteria which is a large group of beneficial bacteria used in certain foods while they are getting prepared such as yogurt, cheese, sour cream, butter milk and other type of fermented milk products. Things such as vinegars are produced by bacterial acetic acid fermentation. Yeast is also major use in the making of beer and wine and also for the leaving of breads. This also involves fermentations to convert corn and other vegetable carbohydrates to also make beer, wine or gasohol but also bacteria is the agents of are other foods. Other fermented foods will include things such as soy sauce, olives and cocoa. (Microbes and human life, 2013) Single cell proteins are known as dried cells of microbes which are used in protein supplement shacks. They are also called “novel food” and “minifood”. The production of this requires micro-organisms which then serve as the protein source and then the substrate which is biomass which they grow on them. There are a number of both these sources that we are able to use for the production of single cell protein (SCP). The micro-organisms used belong to the following groups of Algae, Fungi and bacteria. (Slide Share, 2012)
Prokaryotes include several kinds of microorganisms, such as bacteria and cyanobacteria. Eukaryotes include microorganisms as fungi, protozoa, and simple algae. Virus cells often consist of just a nucleic acid either DNA or RNA in a protein capsule. Viruses are considered neither prokaryotes nor eukaryotes because they lack the characteristics of living things, except the ability to replicate (which they accomplish only in living cells).