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Bioinorganic Coordination Chemistry

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Bioinorganic Coordination Chemistry


This experiment uses H2TTP made during the last lab and hydrated
copper (II) acetate to convert the H2TTP to Cu(TTP). The reaction
proceeds by being refluxed for 30 minutes. The final product is a
non-iridescent purple color, unlike the vivid, shimmering purple color
of the starting H2TTP.

Introduction

Thin layer chromatography, or TLC, is used as a prerequisite for
column chromatography. When performing types of chromatography, like
TLC, the polarity of solvents is extremely important. This lab uses
TLC plates, five solvents (hexane, toluene, ethyl acetate,
dichloromethane, and acetone), and small evaporating dishes to
determine which solvent would be best to use in column chromatography.
The least polar solvent is hexane, which has a polarity index of 0.1.
Toluene has a polarity index of 2.4, while ethyl acetate’s is almost
twice that at 4.4. By comparing the polarity indexes, you can tell
what solvent will separate your dots of product on your TLC plates the
best (Skoog ...


... middle of paper ...


...rs of the neutral complex Cr(CO)4[P(C6H5]2
is loaded onto a silica gel and eluted with CHCl3. Which isomer would
elute first, and
why?
The trans isomer would elute first.

References

Skoog Douglas; Holler F. James; Nieman Timothy A., Principles of
Instrumental Analysis, fifth edition, Thompson Learning: 1998.


UV-Vis Absorption Spectroscopy-Theory.
http://www.shu.ac.uk/schools/sci/chem/tutorials/molspec/uvvisab1.htm.

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