Bioinorganic Coordination Chemistry Essay

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Bioinorganic Coordination Chemistry

This experiment uses H2TTP made during the last lab and hydrated
copper (II) acetate to convert the H2TTP to Cu(TTP). The reaction
proceeds by being refluxed for 30 minutes. The final product is a
non-iridescent purple color, unlike the vivid, shimmering purple color
of the starting H2TTP.


Thin layer chromatography, or TLC, is used as a prerequisite for
column chromatography. When performing types of chromatography, like
TLC, the polarity of solvents is extremely important. This lab uses
TLC plates, five solvents (hexane, toluene, ethyl acetate,
dichloromethane, and acetone), and small evaporating dishes to
determine which solvent would be best to use in column chromatography.
The least polar solvent is hexane, which has a polarity index of 0.1.
Toluene has a polarity index of 2.4, while ethyl acetate’s is almost
twice that at 4.4. By comparing the polarity indexes, you can tell
what solvent will separate your dots of product on your TLC plates the
best (Skoog 761).

Experimental Section

The equipment needed for this experiment is as following: 100 mL round
bottom flask, stir bar, water condenser, hot plate, UV light, Pasteur
pipet, ice bath, separatory funnel, rotary evaporator, evaporating
dish, cotton for filtering, and UV-vis spectrometer.

The chemicals required are: 0.1 g. of H2TTP made previously, 20 mL
N,N-dimethylformamide, 0.16 g. hydrated copper acetate, distilled
water, 75 mL dichloromethane, hexane, toluene, ethyl acetate,
dichloromethane, and acetone for TLC plates.


1. Measure out 0.1 g. of the H2TTP made previously into a 100 mL

... middle of paper ...

...aporates off the gel leaving open sites
where polar molecules can bond. When you let silica gel out in the
open, it will collect water molecules on it. Compounds would elute
faster down a column that has been heated at 150 degrees for 8 hours
because the gel would be dry.

3. A mixture of cis and trans isomers of the neutral complex Cr(CO)4[P(C6H5]2
is loaded onto a silica gel and eluted with CHCl3. Which isomer would
elute first, and
The trans isomer would elute first.


Skoog Douglas; Holler F. James; Nieman Timothy A., Principles of
Instrumental Analysis, fifth edition, Thompson Learning: 1998.

UV-Vis Absorption Spectroscopy-Theory.

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