Rate of Reaction Using Enzymes In this bit of coursework I will be investigating the rate of reaction in which enzymes are the catalyst breaking down a substrate. The substrate I am going to use is Hydrogen Peroxide (H2O2, water with another Oxygen), this molecule usually breaks down on its own accord, but also the rate of decomposition can be increased with the use of a catalyst; in this case the catalyst is a biological one called an enzyme. Hydrogen Peroxide slowly breaks down on its own accord into water (H2O) and oxygen (O2): 2 H2O2 à 2H2O + O2 Hydrogen Peroxide à Water + Oxygen In my experiment, I want to investigate the rate of reaction with an enzyme, and the enzyme I will add to the Hydrogen Peroxide will be Catalase. The enzyme will accelerate the rate of reaction without being used up, for that is a property of a catalyst is, it speeds up a reaction without being used up, and an enzyme is a biological catalyst. [IMAGE] Method Before doing my 'actual' experiment from which I would take my data and ultimately come up with a conclusion I did a preliminary experiment, which was for a number of reasons. Firstly, I had no previous experience of doing this reaction and so needed an idea of how it went: how quickly, how much oxygen was produced and what measurements to use. Also there were two different experiments in which I could have got my data from, one of the experiments was relatively simple and the other more complex but also more accurate. The first experiment I had a choice to do was one in which I had a rack of six test tubes; the procedure I had to follow was simple, I had to put 5... ... middle of paper ... ... * Rate of reaction will double because there will twice as many particles and therefore the chance of a collision between the reactants with enough energy will be double, and therefore the rate of reaction will be twice as quick. This is true until the maximum rate of reaction is reached, an enzyme only works up to a maximum speed. All the data I have collected backs this conclusion up until 50% were things don't seem to fall into place. But in the experiment I did on the first day you can see that it supports my conclusion al the up until 100% concentration. This is true up until the maximum rate of reaction of an enzyme is reached; an enzyme has a maximum rate of reaction and can work proportionally quicker as a factor proportionally increases until it reaches a maximum rate of reaction.
Catalysts: The use of a catalyst can be an advantage however it may generate waste that requires disposal. Some manufacturers develop a catalyst for a specific reaction however this may be time consuming.
Investigation of How the Concentration of Catalase Enzyme Affects the Rate of Reaction Aim: To find out how the concentration of Catalase Enzyme will affect the enzyme activity and the rate of reaction towards Hydrogen Peroxide. (H O ) Prediction: I predict that with the higher concentration of enzyme, the likelihood of it breaking down molecules will be greater because there will be more enzymes to work at the substrate and the chances of it colliding will be higher making the activity time quicker. Equipment: · Syringe · Measuring Cylinder (×2) · Knife · Blender · Beakers (×2) · Balance · Hydrochloric Acid in a beaker · Stop clock · Potato · Water in a beaker Preliminary Experiment: In this experiment we will be using an enzyme called Catalase. By using different amounts of this enzyme we will be diluting it with water to test how the concentration of Catalase affects the rate of reaction with Hydrogen Peroxide.
The rate of reaction of Succinate dehydrogenase. Introduction: Enzymes are protein molecules that function as biological catalysts that can help break larger molecules into smaller molecules while remaining unchanged. They speed up the chemical reactions by lowering the energy of the activation barrier, specific to one molecule. The enzyme’s specificity arises from its active site, an area with a shape corresponding to the molecule with which it reacts (the substrate).
It is important however to note that the NH4 and K ions are still in
The Effect of Changing the Concentration of the Enzyme Catalyst on the Rate of Reaction on Hydrogen Peroxide
Investigating Rates of Reactions My aim is to investigate what factors affect the rate of reaction between magnesium and hydrochloric acid. The factor that I will be focusing on is the concentration of Hydrochloric acid. [IMAGE]Reaction Equation: Mg (s) +2HCl (aq) = MgCl2 (aq) +H2 (g)
Investigating the Rate of Reaction between Amylase and Starch. Plan Aim: To be able to The aim of this investigation is to find out whether the volume of amylase affects the rate of reaction between amylase and starch. Prediction: I predict that the greater the volume of amylase then the faster the rate of reaction between the starch and amylase. I predict this because of the lock and key hypothesis.
cloudy, to test this; I will place a piece of paper with a cross on
Investigating Factors that Affect the Rate of Reaction There are certain factors which affect the rates of reaction in an experiment. These factors are: · Pressure · Temperature · Concentration * Surface area / particle size and * The addition of a catalyst The factors that I will be concentrating on are temperature, concentration and surface area / particle size. Pressure Pressure influences the rate of reaction only when the reactants are in their gas phase. Pressure does not affect them much when they are either solids or liquids.
Investigating a Factor that Affects Enzyme Activity Planning -------- Aim --- To investigate a factor which will affect the activity of catalase, whilst keeping all variables constant. Possible Independent Variables ------------------------------ Here are a number of possible independent variables that could be changed in the experiment: Independent variable Continuous/Discontinuous Easy to measure?
Even before i have done the experiment, I am in a position to make a
Kinetics of Ester Hydrolysis Catalyzed By Imidazole Experiment 3. Ban He Lab Partner: Colton Kincy TA: Ally Fairman September 19, 2014. Abstract: The purpose of the experiment was to study the kinetics of the hydrolysis of ester, p-nitrophenyl acetate (NPA) that is catalyzed by the buffer imidazole (Im).
Rates Of Reaction Investigation Plan: This experiment is planned to investigate the rate of reaction when mixing Copper sulphate (CuSO4) with Zinc powder (Zn). My plan is to see what happens before, during and after the reaction takes place. I will need the following equipment to carry out this investigation: Ø A 50ml marked measuring tube Ø A digital precision scale Ø 600ml of copper sulphate Ø A digital thermometer Ø A pot of Zinc powder Ø A sandpaper (to sand the Copper sulphate off the thermometer) Ø A pen and a paper (to record results.)
Investigation of Rates of Reaction I am going to investigate the rate of reaction with magnesium and
To control the rates of chemical reactions is imperative to the continued existence of our species. Controlled chemical reactions allow us to move forward in society, constantly. We find new ways to provide light and heat our homes, cook our food, and pursue in crafts that benefit our society. There are, however, just as there are advantages, disadvantages to the efficiency of controlling the rate of reactions, which in some cases can be fatal to our scientific development and progression. The growth of humankind necessitates that we must be able to control the rate of chemical reactions.