Introduction :-
Many children around the world , especially in third world countries suffer many bacterial infection of the gastrointestinal system as a result of lack of awareness and the spread of the disease in the less attention to hygiene and the environment . In order to explain this word gastrointestinal tract infection , especially the stomach and intestines , it can result from infection by bacteria or virus or other parasites (1) . Some of the causes of inflammation as a result of eating food poisoning , especially meat and eggs . However , some symptoms of severe cases are headache , nausea , vomiting , general weakness , diarrhea and pain , the illness begins suddenly with high fever , vomiting , abdominal pain and stool liquid to water that may contain some blood and mucus . The result of drought through loss of fluids and salts and lead an alliance with the poisoning to the collapse of the circulatory system in diseases such as cholera (2) . It must be conducted in diagnosing bacterial to find the source of infection because it causes illness and death of millions in the third world and in particularly children as mentioned . In this report will refer to the most common types of bacteria cause gastrointestinal tract infection for example , Shigella , Salmonella , Vibrio and Campylobacter (3) . First of all , Shigella is gram negative , nonmotile , non lactose fermenting and H2S production . There are four important species , S. sonnei , S. flexneri , S. boydii and S. dysenteriae is the most serious . Next is Salmonella which is gram negative as well , non lactose fermenting and production H2S . These species are S. typhi and S. paratyphi found in humans and animals . Finally , Campylobacter is gram negati...
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...ies . These results were indicated to C. jejuni which is pathogenic bacteria .
Case study 4:
Passenger was 68 years old , who travelled from Europe to Melbourne via flying to Middle East , Asia and Singapore . When he arrived he was hospitalized . The specimen sent to laboratory show that much growth and yellow colonies on TCBS , oxidase was positive . Furthermore , other tests which is confirmed this result is ability to grow in NaCl 1 % and without NaCl bottles , it was given turbid growth , as seen in susceptibility test was about 0 / 129 zone of inhibition which is sensitive . While in polymyxin B antibiotic was no zone which is resistance . These outcome were indicated to Vibrio . To identify between two species V. cholera and V. mimicus via views these colonies color , which is V. cholera given yellow while V. mimicus given blue to green color .
In this lab project, the microbiology students were given 2 unknown bacteria in a mixed broth each broth being numbered. The goal of this project is to determine the species of bacteria in the broth. They had to separate and isolate the bacteria from the mixed broth and ran numerous tests to identify the unknown bacteria. The significance of identifying an unknown bacteria is in a clinical setting. Determining the exact bacteria in order to prescribe the right treatment for the patient. This project is significant for a microbiology students because it gives necessary skills to them for future careers relating to clinical and research work.
The purpose of this study is to identify an unknown bacterium from a mixed culture, by conducting different biochemical tests. Bacteria are an integral part of our ecosystem. They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species. These biochemical tests are process of elimination that relies on the bacteria’s ability to breakdown certain kinds of food sources, their respiratory abilities and other biochemical conditions found in nature.
The results of the gram stain test were cocci and purple. This indicated that the unknown bacteria were gram positive. The gram stain test eliminated Escherichia coli, Klebsiella pneumonia, Salmonella enterica, and Yersinia enterocolitica as choices because these bacteria are gram negative. Next a Blood Agar plate was used because in order to do a MSA or a Catalase test there needs to be a colony of the bacteria. The result of the Blood Agar plate was nonhemolytic.
The purpose of this laboratory is to learn about cultural, morphological, and biochemical characteristics that are used in identifying bacterial isolates. Besides identifying the unknown culture, students also gain an understanding of the process of identification and the techniques and theory behind the process. Experiments such as gram stain, negative stain, endospore and other important tests in identifying unknown bacteria are performed. Various chemical tests were done and the results were carefully determined to identify the unknown bacteria. First session of lab started of by the selection of an unknown bacterium then inoculations of 2 tryptic soy gar (TSA) slants, 1 nutrient broth (TSB), 1 nutrient gelatin deep, 1 motility
Trabelsi, H., Dendana, F., Sellami, A., Sellami, H., Cheikhrouhou, F., Neji, S., … Ayadi, A. (2012). Pathogenic
The purpose of this project was to identify unknown bacteria species from a mixed culture. The two unknown species were initially plated onto Tryptic Soy Agar (TSA), Eosin Methylene Blue (EMB), Mannitol Salt Agar (MSA), and blood agar plates to distinguish between the two different bacteria using colony size, color, shape, and growth characteristics. By identifying and inoculating the differing types of colonies, the two unknown bacteria were purified and able to be tested
Abstract: Salmonella is a bacterium that causes one of the most common intestinal infections in the United States - Salmonellosis. The chance of contracting this disease is significantly high, and more and more people are suffering from the symptoms and complications of Salmonella. This paper will discuss about the disease itself, the current outbreaks that are related to this disease, preventions and the treatments.
Necrotizing Fasciitis (flesh eating bacteria ) from an essay by Katrina Tram Duong, edited by S.N. Carson M.D.
Salmonella is a gram-negative rod, which is known to infect humans, birds, and other mammals by invading and irritating the intestines. It is more common in poultry and swine which, if consumed, can cause diarrhea, nausea, vomiting, and stomach cramps. The infectious disease can be found almost everywhere and anywhere, including in water, animal feces, raw poultry, and raw seafood. Animals consume salmonella from the soil or contaminated processed feed. In humans, salmonella can lead to a developing typhoid-like fever that persists among other symptoms. However, there is no vaccine available for Salmonella but no treatment is needed because the infection is usually simply fought off by the immune system. There are various ways of preventing the salmonella infection as well, for example, careful cleaning of hands and utensils while cooking are simple preventative actions. Keeping clean and properly preparing food are the best ways contamination and spread of Salmonella can be avoided.
In the Nepal’s example on the web, the child sickness was due to lack of education on food hygiene and lack of infrastructure (sanitation and drinking water). Thus, teaching the mother about treating water before drinking and the importance of hygiene in handling aliments would prevent the child diarrhea episode and even save the other son that died in the past from this disease.
Norovirus is one of the leading causes, if not the leading cause of gastroenteritis worldwide (6).
The patient has experienced fever, chills on body, headaches and anorexia as well as sweating especially during the night. The patient has also been feeling fatigued, muscle aches and nausea as well as vomiting especially after eating (WHO, 2010, p. 117). These symptoms started forty eight hours ago, and the patient has not taken any medication except for some aspirin.
Cholera is still an extremely significant disease worldwide with over 5 million cases being reported per year (Ruiting & Reeves, 2002). Cholera is a diarrheal illness that progresses rapidly and is contracted by ingesting the bacterium Vibrio cholerae which causes an intestinal infection (CDC, 2013). In many cases the illness is mild with hardly any symptoms at all, but in some cases it can become severe. Approximately 5 percent of people who are infected exhibit severe symptoms such as extreme watery diarrhea, leg cramps, and vomiting (CDC, 2013). These symptoms usually occur at a rapid pace and unless treated can further lead to dehydration and shock which can ultimately cause death within hours. It is estimated that over 100,000 deaths occur each year around the world due to Cholera. (CDC, 2013)
Malnourished children acquire bacterial gastrointestinal and respiratory infections more frequently. 42. Malnutrition significantly compromises mucosal epithelial barriers in the gastrointestinal, respiratory and urogenital tracts which are first line of defense against infections.
Food borne illnesses are caused by consuming contaminated foods or beverages. There are many different disease-causing microbes, or pathogens. In addition, poisonous chemicals, or other harmful substances can cause food borne illnesses if they are present in food. More than two hundred and fifty different food borne illnesses have been described; almost all of these illnesses are infections. They are caused by a variety of bacteria, viruses, and parasites that can be food borne. (Center 1)