This study demonstrates a facile, one−pot preparation of casein hydrolytic peptides (CHPs)–conjugated crystalline AuNPs having average core size about 20 ±10 nm and morphology found to be hexagonal shaped. The CHPs are competent to form the monolayer on the AuNPs surface resulting electrostatic interparticles interaction, and plays an important role in stability for long periods of time (12 months). The X−ray photoelectron (XPS) spectrum, showed a strong peak for the pure ‘Au’ phase. The FTIR spectrum indicated that the electrostatic capping of the CHPs, which drives the formation of organic−inorganic CHPs−AuNPs composite. The CHPs−AuNPs establish low viability towards the human prostate cancer (DU145) cell line as compared to normal mouse hepatocyte (NCTC1469) cell line, under identical culture conditions. For that reason, DU 145 cells are particularly susceptible to CHPs−AuNPs, which is essentially required for the exploring their anti−cancer applications. Thus, CHPs−AuNPs were revealed as the ideal applicant for the treating cancer cells and other biomedical application.
1. Introduction
The nano-biotechnology research field shown fast growth over the past decades, utilizing gold nanoparticles (AuNPs) in biological applications, due to their unique surface, optical and electronic properties [1,2]. Thus, new research in nano-synthesis, design, and functionalization of AuNPs have to, improve their efficiency, bio-compatibility, and find new applications to mature the nanotechnology discipline. Several scientific reports have appeared in the last decade that are highlighting the cytotoxicity of AuNPs [3,4]. Some researchers have been demonstrated that the different toxicity pattern of AuNPs obtained from different...
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... situ synthesized discrete Au nanoparticles. Langmuir 2012; 28: 5947−5955.
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4. Chromium Picolinate Toxicity; by, Cerulli J., Grabe DW., Gauthier I., Malone M., and McGoldrick MD.. ANNALS OF PHARMACOTHERAPY. 32(4) : 428-31, 1998 April
...e materials at the nanoscale might include new form of nanobase toxicity. The individuals working with large quantity of nanomaterials need to take appropriate measures to avoid inhalation and ingestion. However, scientists have found silicon o be good for the construction of nanorobots because of its unique properties; durability, flexibility and conductivity. However, silicon cannot dissolve in body fluids. In addition, in medical applications biodegradability is going to be challenge due to the foreign particles inside the body and controlled mobility.
In this case study, our concern goes for the chitosan nanoparticles; firstly nanoparticles are able to adsorb and/or encapsulate a drug, thus protecting it against chemical and enzymatic degradation. Furthermore the encapsulated drug may be prevented from crystallization, thus forming a solid solution. Depending on drug solubility in the carrier, a drug load varying from only a few percent up to 50%] Secondly, chitosan is ...
They have a larger surface area in proportion to their volume. This enables them to interact with different types of biological systems and provides a wide variety of possibilities (Nuruzatulifah, Nizam, & Ain, 2015). The nanoparticles can be seen by transmission electron microscopy (TEM). When there is one nanoparticle, it is called a primary particle. When there is more than one, it is called a secondary particle. In order to measure these particles, they need to be suspended in a solution (Pruneanu, Coros & Pogacean, 2015). Dyed nanoparticles or internally fluorescent nanoparticles barely interact with cellular proteins which is what the study requires. They are also quite easy to manipulate. They can be easily internalized into cells and can be programmed to go to specific sites (Wolbeis,
According to a critical review by Rosenblatt et al (2009), “.silver interacts with sulfhydryl groups of proteins and with DNA, altering hydrogen bonding and inhibiting respiratory processes, DNA unwinding, cell-wall synthesis, and cell division (Pg #? ). ” It is through those means that pathogens are destroyed and biofilm formations are hindered. The study by Lou et al (2011) shows how SDF reacts with different tooth structures.... ... middle of paper ...
I am going to discuss about the element known as gold in my assignment. To describe the element gold in simple terms, I can only say that it is an element (chemical element). This element is denoted by the symbol Au. It has an atomic number of seventy nine (79). I will describe quite a number of things concerning gold as an element. To begin with is:
They found that surface bacterial counts were significantly diminished when treated with nanocrystalline dressing. Bacteria are known to damage tissue leading to poor healing, thus lower bacterial load as a result of silver nanocrystalline dressing, facilitates wound healing, while growth factors are crucial in healing, as they attract cells necessary for epithelial cell migration and fibroblast growth and they initiate the formation of new blood vessels in the injured area. [14] Moreover, studies on a mouse model reveal that wounds treated with silver nanoparticle dressings resemble more to normal skin when it comes to collagen configuration and tensile properties, compared to untreated control
Almost everyone is touched by cancer in some way and the number of people living with and beyond cancer grows greater every year. Globally 14 million people are diagnosed with cancer each year and 8 million people will die from it annually. Half of all men and one-third of all women will develop cancer during their lifetime. (13) There are many treatments for cancer, mainly: surgery, radiation and chemotherapy. These traditional treatments have many negative side effects. Therefore, increasingly, other treatments, such as hormonal therapy and targeted therapy are being used for certain cancers. Nanotechnology is a form of targeted therapy that destroys cancer tumors with minimal damage to healthy tissues and organs. Scientists are already using nanotechnology in early detection of elimination of cancer cells before they form tumors. But the real game changer will be when nanotechnology targets cancer tumors in treatment (11,13).
In “Trap of Gold”, Louis L’Amour develops the theme, “ greed and ambition can be harmful”, through the use of Wetherton and the mountain. First, Wetherton loves his family greatly and values them above all things. He had been gone from Horsehead and his family, his wife Laura and his little boy Tommy, for some time looking for gold before he found any trace of it. “Wetheron had been three months out of Horsehead before he found his first color” (L’Amour 149). Later, as the story progresses and Wetherton continues to mine, he becomes aware that greed and the need for gold is controlling him, and the base of the structure is weakening, putting himself in more danger with every chunk of quartz he removed with his pick. “Now the lust of gold was getting to him, taking him by
Research done by Figueroa et al. (2013) has turned up an advance in the method of non-viral gene delivery utilizing gold nanoparticles (AuNP). In their work they have developed an exceptionally efficient way of transfection by experimentally improving certain desirable traits of the gold nanoparticle polyamidoamine (AuPAMAM) c...
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
HEALTH EFFECTS OF CRYSTALLINE SILICA EXPOSURE Description of Silicosis When workers inhale crystalline silica, the lung tissue reacts by developing fibrotic nodules and scarring around the trapped silica particles [Silicosis and Silicate Disease Committee, 1988]. This fibrotic condition of the lung is called silicosis. If the nodules grow too large, breathing becomes difficult and death may occur. Silicosis victims are also at high risk of developing active tuberculosis [Myers et al.].
25 April 2014. Muller, F. Toxicological Sciences. 2014. The 'Standard' of the 'Standard'. 24 April 2014. National Cancer Institute.
Regenerative medicine targets the conservation and development of organ function. The modification of tissues is obtained by linking living cells with materials that work as scaffolds to support cell generation. Nanotechnology is the means that feeds the material structure that simulate biological ones and additionally provide a direct delivery system. The functionality of nanotechnology for regenerative medicine is primarily due to its small unit size according to the International System of Units, one nanometer is one-billionth of a meter. The unit size allows for the technological apparatus to navigate the bloodstream and target and fix damage cells in tissues.
Enhanced Nutrient Delivery: Nano-encapsulating improves the solubility of antioxidants, vitamins, healthy omega oils and other neutraceuticals