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Essay on importance of influenza vaccines
Influenza vaccine case study
Essay on importance of influenza vaccines
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Influenza viruses existing in birds continue to be a source for a diverse combination of antigenic subtypes including 16 hemagglutinin (HA) and 9 neuraminidase (NA) and represent a large reservoir of novel antigens to which the human population is naïve [1,2](1)) (Seasonal influenza epidemics are a major public health concern, accounting for five million severe cases worldwide [1](4))(Annually, influenza types A cause human outbreaks responsible for substantial mortality and morbidity, particularly in high risk groups, such as infants, elderly, and immunocompromised individuals.(5)).(3)) (The influenza virus is one of the most devastating viral diseases due to being highly contagious which easily spreads as an aerosol and causes acute viral respiratory disease and mortality to susceptible groups. In order to prevent the spread of seasonal or pandemic outbreaks of influenza, vaccination is a powerful and cost-effective means [1](15)) (Protection against influenza virus is primarily mediated by antibodies to the viral hemagglutinin (HA) [2,3]) ,HA is the major surface glycoprotein of the virion and responsible for the attachment and penetration of viral particles into cells during the initial stages of infection.((5)((8)) (Successful prophylactic influenza vaccines elicit efficient HA-specific systemic antibody, which can bind the virus and inhibit early events in the influenza virus infection.(6)) Different types of influenza vaccines such as subunit [7-10], attenuated [11,12], and inactivated influenza vaccines[14] are available although the inactivated ones are the most widely used in the commercial scale [6]. ((12) (the major substrate for the preparation of inactivated influenza vaccines is embryonated chicken’s egg .((1) (In c...
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... the P1 baculovirus stock In Sf-900 III Medium was Prepared, as appropriate. To do this, sequentially 0.25 ml of the baculovirus stock was diluted in 2.25 ml of Sf-900 medium. The dilutions 10–4 to10–8 were used in our assay. The medium from each well removed, immediately replace with 1 ml of the appropriate virus dilution and incubated for 1 hour at room temperature.
Plaquing medium containing 12.5 ml 4% Low Melting Agarose and 37.5 ml Sf-900III was prepared and incubated at 40˚C water bath until use. Following the 1 hour incubation, the medium containing virus from the wells removed and replaced with 2 ml of plaquing medium.
Allowed agarose overlay at room temperature until to harden. The plates incubated at a 27˚C humidied incubator for 7–10 days. To improve the visualization of plaques, the plates were stained by 0.5 ml Neutral Red solution (1 mg/ml). (21)
After 48 hours of incubation the agar plates were viewed. Individual colonies were tested for successful isolation by gram staining and then viewing the stained bacteria under a microscope. Isolation was successful. One colony of each unknown bacteria was transferred to an agar slant for growth. The agar slants were stored at room temperature over the weekend so that they would not grow too much.
Streak plate technique was used to isolate pure culture for each bacteria (2). The Gram stain was used to determine Gram reaction and morphology of each bacteria (2) Selective and differential media such as, salt agar, MacConkey agar and blood agar were used for bacterial identification (2). Gelatin deeps were inoculated to detect production of gelatinase (2). Starch Agar plate were inoculated to detect amylase (2). Ocular reticle used to determine bacteria size (2). Motility deeps were inoculated to detect motility on bacteria (2). Thioglycollate broth used to determine oxygen requirements (2). Carbohydrate fermentation
coli cells were grown at 37 degrees Celsius with calcium ions. These cells were then incubated in ice and placed into 4 polypropylene tubes. Each tube consisted of 25 microliters of E.coli cells. Two of the four were labeled with (+) while the other two were labeled (-). Positive was with samples that contained 100 pg/ microliter of pGLO, while negative was for the samples that did not. The 4 tubes were incubated in ice for 30 minutes, then heat shocked for 30 seconds at 42 degrees Celsius. The 4 tubes were then returned to the ice bath for 5 minutes. After the removal, .975 microliters of LB media was added to each tube at room temperature. The tubes were then shook at 225 RPM at 37 degrees Celsius for one hour. 300 microliters of cells were then extracted from the tubes and then placed in agar plates. The cells were spread across the plate and the incubated overnight at 37 degrees
However due to globalization, import and export viruses is more easily transmitted. Over the past century the global community especially Asian has been affected with new strains of the influenza virus. The changes in the virus can occur in two ways “antigenic drift” which are gradual changes in the virus over time. This change produces new strains that the antibody may not recognize. “Antigenic shift” On the other is a sudden change in the influenza virus which ‘’ results in a new influenza A subtype or a virus with a hemagglutinin or a hemagglutinin and neuraminidase combination that has emerged from an animal population,” as seen with H5N1 virus. This change leaves people defenseless against this new virus. (CDC, 2013) Currently there is no vaccine to combat all strains therefore “Planning and preparedness for implementing mitigation strategies during a pandemic requires participation by all levels o...
We have aimed to generate escape mutants under the selection of our highly potent neutralizing antibody F10 which has been extensively characterized for structural insights into the mechanisms of epitope-specific neutralization. This F10 antibody is particularly valuable for the immune-driven viral evolution studies as this antibody targets highly conserved pocket in the stem region shared with the diverse influenza subtyupes and function critical for viral fusion. Therefore, characterizing permitted evolutionary routes of the virus over the course of F10-type immune selection may serve as templates for the design of universal influenza vaccine and treatment strategies against all types of influenza viruses including those emergent pandemic strains. Selection of VN/04 (H5N1) or A/PR/8 virus escape mutants with F10 antibody was performed in MDCK cells under conditio...
Transfer 100 µL of cells from the “-plasmid” tube to the agar plates marked with “LB/Amp -plasmid” and “LB -plasmid.” Take an inoculating loop and bend one of the ends into a 90 degree angle so that the loop now resembles a hockey stick. Use the bent inoculating loop to spread the cells along the plate’s surface while making sure that you are not piercing the agar’s surface. Repeat the same process when transferring cells from the “+plasmid” tube to the plates labeled “LB/Amp +plasmid” and “LB +plasmid.”
Current influenza vaccines are about 70% to 90% effective in preventing influenza in healthy adults. Since the vaccines are made of dead fragments of influenza viruses, they cannot cause influenza. The strains of influenza that circulate change every year and therefore, it is necessary to make a new influenza vaccine annually. After vaccination, the body's immune system produces antib... ... middle of paper ... ...
The virus is primarily spherical shaped and roughly 200nm in size, surrounded by a host-cell derived membrane. Its genome is minus-sense single-stranded RNA 16-18 kb in length. It contains matrix protein inside the envelope, hemagglutinin and neuraminidase, fusion protein, nucleocapsid protein, and L and P proteins to form the RNA polymerase. The host-cell receptors on the outside are hemagglutinin and neuraminidase. The virus is allowed to enter the cell when the hemagglutinin/ neuraminidase glycoproteins fuse with the sialic acid on the surface of the host cell, and the capsid enters the cytoplasm. The infected cells express the fusion protein from the virus, and this links the host cells together to create syncitia.
Loo, Yueh-Ming and Michael Gale, Jr. “Influenza: Fatal Immunity and the 1918 Virus.” Nature 445 (2007): 267-268. 23 July. 2008 .
The influenza virus is an enveloped virus that contains a genome of eight genes that define what the virus is. Everything begins when the virus enters the airways. Here, influenza viruses specifically attach to the surface of epithelial cells. The viral membrane envelope contains the neuraminidase (NA) protein, which is important for the efficient release of newly produced viruses. It also contains the matrix 2 (M2) ion channel that promotes viral structural changes during cellular entry as well as the influenza hemagglutinin (HA) protein, the key player for viral internalization, which facilitates viral binding to sialic acid decorated receptors on host cells, causing adsorption to the host cell (Samji, p. 3). Barry compares the HA proteins to little spikes and the NA proteins to tiny trees that both protrude all around the surface of the virus (p. 103). When the HA protein spikes come into contact with the sialic acid molecules, both structures bind to one another. Once this binding holds the virus and host cell together, the virus has achieved its first task of adsorption. Next, the virus particles are internalized into endosomes by clathrin mediated endocytosis. The pH of the endosomes drops tr...
Influenza is a major public health problem which outbreaks all over the world. Resulting in considerable sickness and death rates. Furthermore, it is a highly infectious airborne disease and is caused by the influenza virus. Influenza is transmitted easily from one person to another person which has a great impact on society. When a member of society becomes sick, it is more prone to spread to other people. In the United States, every year between 5 to 20 percent of the population is affected by influenza. As a result of this, between 3,000 and 49,000 deaths have occurred per year (Biggerstaff et al., 2014). Therefore, the influenza vaccine is the most effective strategy to prevent influenza. This essay will examine two significant reasons for influenza vaccination which are the loss of workforce and economic burden as well as one effect regarding herd immunity.
“Selecting the Viruses in the Seasonal Influenza (Flu) Vaccine.” Centers for Disease Control and Prevention. USA.gov, 9 March 2011. Web. 19 Jan. 2010
This experiment was done by using a series of methods that allowed for fluorescent labeling, immunofluorescence, and fluorescence imaging. The viruses were labeled with DiD which allowed for visualization in the experiments. ……. Florescence imaging was used to obtain multiple images of the DiD labeled viruses. This was accomplished by using a laser to excite the tags. Green and red dyes can be seen together or separately depending on the wavelength used during viewing. The images were analyzed by determining peaks.
In order to decide whether or not the swine flu vaccine is completely necessary, one must first gain a better understanding of the topic. It is a scientifically known fact that the swine flu is a result of a virus. A virus is a capsule of genetic material that causes infection in the body. The infectious particles are made up of nucleic acid enclosed in a protein shell, called a capsid. It cannot be considered a living organism like the disease causing agent of bacteria, because it does not carry out all the characteristics of life. Specifically, it cannot reproduce on its own.
Influenza viruses are constantly evolving due to the mechanism of antigenic drift. This results in seasonal vaccination to target only specific strains, which puts us in a race against the clock in the prevention of the next pandemic. One key to solving this is the development of a universal influenza vaccine, which would elicit a broad antibody response. This would target either multiple strains or strains from the past, present, and future in a single vaccination. As vaccine may target sites such as the neuraminidase (NA) or the M2 Ion channel, hemagglutinin (HA) is preferred by most approaches due to the consensus amino acids found throughout the different subtype, specifically the stem region (3). However, there are boundaries to the stem approach, such as, that some antibodies have reduced affinity for the stem region (1).