Flagellar protein mutations determine the movement and regeneration of flagella in Chlamydomonas reinhardtii

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INTRODUCTION

Chlamydomonas reinhardtii is a unicellular, eukaryotic green algae that is commonly found throughout the world. This photosynthetic organism possesses two flagella that serve as the basis of its motility. Not only is this organism easily accessible, but it is a model organism for many areas of study (Rochaix et al, Silflow and Lefebvre 2001) including photosynthesis, respiration, flagella, circadian rhythm, cell to cell recognition and even heavy metal homeostasis and tolerance (M. Hanikenne). The flagella of C. reinhardtii are easily visible and are remarkably similar to other mammalian microtubule structures (Silflow and Lefebvre 2001). This allows experimental studies to occur that are able to aid in the understanding of human disease related to the dysfunction of microtubule structures within the human body. Since these organisms are capable of sustaining life in absence of their flagella, they become a model organism for studying movement, regeneration and mutational defects. The flagella are easily removed without damaging the cell and make flagellar protein analysis a simpler process. Genetic analysis of mutant strains is easily accessible because of the complete sequencing of its genomes (Lefebvre and Silflow 1999).

Although flagellum does not have a life-sustaining role in this organism, it is still very beneficial throughout its lifecycle. Like all chlorophyll producing plants, C. reinhardtii requires light to support its cellular functions. In order to enhance its ability to receive the ideal quantity, it is able to sense light with an eyespot and use these signals to control the beating of the flagella (Witman GB 1993). C. reinhardtii is able to propel itself forward by the opposite and simultaneous mo...

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