Estrogens

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Estrogens are among the most important hormones in the female body, which pass through distant organs, interact with multiple organ systems, and play a pivotal role in the physiologic events that occur during a woman’s life. [1] Presence of endogenous hormones in the plasma poses challenges for the pharmacokinetic and bioanalytical considerations in terms of endogenous baseline levels, low plasma concentration, analytical range, and possible effect of circulating hormones. In order to quantify plasma concentrations of estrogens in clinical trials, it was necessary to develop and validate an assay with appropriate sensitivity, selectivity, accuracy and precision. [2]

OBJECTIVE:

The objective of the present study is to develop the more sensitive, rapid and simultaneous quantification of estrogen i.e. Estradiol (E2) and Estrone (E1) in human plasma.

EXPERIMENTAL METHODS:

1. Chemicals: The pure substances of Estradiol, Estrone were procured from the council of Europe, (Strasbourg, Cedex). Amlodipine besilyate (internal standard) procured from Sigma-Aldrich. Dansyl chloride wa...

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