The Effects of Temperature on the Rate of Clotting Milk and Rennet
Introduction
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The following experiment investigates the effects of different
temperatures on a mixture of rennet and whole milk. On having the
choice between testing the mixtures reactions at various temperatures,
or testing the mixture with various amounts of concentration of
rennet, my partner and I decided upon the first option. We made this
decision as we felt it would be valuable to our scientific knowledge
if we had a better understanding of how different temperatures can
effect the behaviour of an enzyme, such as Rennin, which is also known
as Chymosin. Our scientific knowledge tells us that enzymes work most
efficiently at specific temperatures, and this experiment helps us to
discover exactly which temperatures they are.
It is important to remember that the Rennet was mixed with milk, which
is perhaps one of the most important sources of nutrition in the
world, and drunk by billions of people everyday. It is particularly
important to babies and growing children. It provides:
· Calcium, to build strong bones and teeth
· Protein, to build and repair muscle tissue
· Potassium, to help regulate our body's fluid balance
· Vitamin A
And many other useful vitamins and nutrients which help to maintain a
healthy body.
As wonderful a necessity that milk is, it is also an extremely
perishable food. Milk is usually stored in the fridge, because it
preserves better at a low temperature, but even so, once it has passed
its sell by date, it is no longer suitable for consumption. Although
the milk itself does not have a very long life, other foods and some
dairy products can be made using it. Cheese would be the main example
of this, which can be produced simply by the curdling of milk. Rennin,
found in the substance rennet, is a milk-coagulating enzyme capable of
assisting in the production of cheese. Therefore the temperatures at
which the milk and rennet coagulate best at in this experiment, are
After conducting this experiment and collecting the data I would have to say that the optimal temperature for enzyme activity would have to be room temperature which in my experiment was thirty-four degrees Celsius. I came to this answer because the glucose test strip showed that at room temperature there was more glucose concentration that at either of the other temperatures. Due to temperature extremes in the boiling water the enzymes could no longer function because the breakdown of lactose stopped. The cold water also hindered the breakdown of the lactose but as the water warmed the enzymes were more active which can be seen in the results for the cold water at 20 minutes B. Describe the relationship between pH and the enzymatic activity of lactase.
However, once it reaches 70% of concentration, the enzyme becomes saturated, meaning that there are no active sites for the substrates to fill, which leaves casein (milk) molecules suspended in the curd; the saturation point for this curve was located at 6.5x 10-3 seconds. This was clearly evident in some of the visual results of the practical, where we could see that there was still milk that could be decanted when the curd was poured out. Therefore, even if we added more casein substrate, the curve enzyme activity would still flatten, indicating again that all or most of the active sites of the renin enzyme were full.
For example, incubating the samples at different temperatures would create more data points to establish an optimal temperature. From the results in the experiment in this study, it is known as temperature increases, enzymatic activity increase, and vise versa. However, what can not be observed is at what point does the increase in temperature begin to denature the enzyme, above 60°C. Furthermore, assays can be preformed to determine optimal pH, as well. From Dutta’s, and his partners, experiment it shows that there is a range where the Heliodiaptomus viduus’s lactase shows the most activity, which is between 5.0 and 6.0
The Effect of Temperature on the Activity of Rennin in Milk Aim: To find out what effect different temperatures have on the enzyme, rennin, in milk. Introduction An enzyme is a biological catalyst. It speeds up a reaction by lowering the activation energy required to start the reaction. It speeds up a reaction, but remains unchanged unless certain limiting factors are introduced.
Animal metabolism consists of the utilization of nutrients absorbed from the digestive tract and their catabolism as fuel for energy or their conversion into substances of the body. Metabolism is a continuous process because the molecules and even most cells of the body have brief lifetimes and are constantly replaced, while tissue as a whole maintains its characteristic structure. This constant rebuilding process without a net change in the amount of a cell constituent is known as dynamic equilibrium (Grolier1996). In the combustion of food, oxygen is used and carbon dioxide is given off. The rate of oxygen consumption indicates the energy expenditure of an organism, or its metabolic rate (Grolier1996).
Using a Bunsen burner, tripod and beaker of water 100 degrees could also be tested and 0 degrees was tested by using ice. (I didn’t investigate the 80 degrees temperature). Fair test: Below is a list of things that were kept the same throughout the investigation: Volumes of lipase and milk (by using syringes); volumes of phenolphthalein and sodium carbonate (using pipettes); (best volumes from the preliminary work were used). Each temperature was repeated three times to get a good average. The milk and lipase were equilibrated to the right temperatures before the lipase was added to the milk.
The purpose of the lab was to show the effect of temperature on the rate of
Input variables In this experiment there are two main factors that can affect the rate of the reaction. These key factors can change the rate of the reaction by either increasing it or decreasing it. These were considered and controlled so that they did not disrupt the success of the experiment. Temperature-
Cheese is a very famous food that is can be used in many dishes for example main course and desserts. Cheese is a food that is made from a milk based. This milk can come from various sources for example cows and goats. Water content is remove fully from the milk. The function of this process is to improve and concentrated the protein, fat and other nutrients in the cheese. It also use to increase the life time for the cheese. The cheese making is one of the examples for the biotechnology.
Investigating the Effect of Temperature on the Fermentation of Yeast To fully investigate the effect of temperature on the rate of fermentation of yeast Background Information Yeast is a single-cell fungus, occurring in the soil and on plants, commonly used in the baking and alcohol industries. Every living thing requires energy to survive and through respiration, glucose is converted into energy. There are two types of respiration available to living cells are: 1.
NOTE: The stirring rod was not used in the First and Second experiments, as it was not available. A substitute we used the thermometer.
What Makes Human Milk Special? (Mar-Apr 2006). New Beginnings Vol. 23 No.2 , pp 82-3.
There is a great promise of application of ice structuring proteins in food that are frozen. Ice structuring proteins can inhibit re-crystallization during freezing storage, transport and thawing, thus preserving food texture by retarding cellular damage and minimal loss of nutrients (for fruit and vegetables like strawberries, raspberries, tomatoes) by reducing drip (Griffith and Ewart, 1995, Feeney and Yeh, 1998, Breton et al., 2000, Wang, 2000). Re-crystallization in frozen food occurs when temperature fluctuates during storage or transit, resulting in coarse texture. This technique is well suited to ice cream (Warren et al., 1992). Ice structuring proteins also find use in chilled and frozen meat, where large ice crystals may form intracellularly, resulting in drip and loss of nutrition during thawing. Since ice structuring proteins are located extracellularly in freeze-tolerant organism, these proteins can be added to food by physical means such as mixing, injection, soaking, or vacuum infiltration, or even by gene transfer (Venketesh and Dayananda, 2008).
The pH of the solution would alter the rate of the reaction if it was
How Temperature Effects the Movement of Pigment Through Cell Membranes Abstract The experiment below displays the effects of temperature on the pigment in uncooked beetroot cells. The pigment in beetroot cells lies within the cell vacuole and is called anthocyanin, each vacuole is surrounded by a tonoplast membrane and outside it, the cytoplasm is surrounded by the plasma membrane, therefore the foundation of this experiment lies with the temperature at which the membranes will rupture and therefore leak the pigment. To do this a series of uncooked beetroot cylinders will be exposed to different temperatures and then to distilled water at room temperature (24ºC). The colour of the distilled water is the variable here which will show us, using a colorimeter what temperature the membranes splits using the transmission of the water (light passing directly through and the absorbency (light getting absorbed by the anthocyanin molecules).