The Effect of the Enzyme Catalase on Hydrogen Peroxide When the Temperature is Changed
Plan:
Chemical reactions take place in all living cells. All of these
reactions are enzyme-driven. Some of these reactions produce
by-products. One by-product is Hydrogen Peroxide (H2O2). It is a
poison and must be removed from the cells. The cell produces an enzyme
called Catalase to break down the Hydrogen Peroxide into water and
oxygen. This experiment will investigate the effect of temperature on
the rate of the breaking down of Hydrogen Peroxide by Catalase. I will
do this by measuring how much oxygen is produced when Catalase and
Hydrogen Peroxide react.
I predict that as the temperature increases, the rate of the reaction
will increase. I think that when the temperature is doubled, so the
reaction rate will also double. This is due to the particle theory. As
the temperature rises, the particles move faster and collide more
often. This gives the "lock and key" reaction more chance to occur.
Therefore, as it is a temperature coefficient with a Q10 of 2, if the
temperature is raised by 10ºC, the particles will move doubly fast,
collide twice as often and the rate of reaction will double. There
will be a point when this relationship isn't true. This will be at
around 60ºC when the enzyme denatures.
To make this a fair test, we need to keep the same all variables that
could affect the outcome except for the one being tested, temperature.
So the pH, amount of solutions used, concentration of solutions,
apparatus etc. must all remain the same.
As my source of Catalase, I will use potato. This is easier to cut and
measure than other sources such as liver, which would be hard to cut
into equal pieces. The potato "chip" will be pre-warmed in a boiling
tube in a water bath of each temperature for 10 minutes, as will 20cm3
of H2O2. Once the desired temperature is reached, the chip and H2O2
will be mixed in the boiling tube, shook a couple of times and placed
Investigation of How the Concentration of Catalase Enzyme Affects the Rate of Reaction Aim: To find out how the concentration of Catalase Enzyme will affect the enzyme activity and the rate of reaction towards Hydrogen Peroxide. (H O ) Prediction: I predict that with the higher concentration of enzyme, the likelihood of it breaking down molecules will be greater because there will be more enzymes to work at the substrate and the chances of it colliding will be higher making the activity time quicker. Equipment: · Syringe · Measuring Cylinder (×2) · Knife · Blender · Beakers (×2) · Balance · Hydrochloric Acid in a beaker · Stop clock · Potato · Water in a beaker Preliminary Experiment: In this experiment we will be using an enzyme called Catalase. By using different amounts of this enzyme we will be diluting it with water to test how the concentration of Catalase affects the rate of reaction with Hydrogen Peroxide.
The Effect of Temperature on the Action of Peroxidase Enzyme Aim To find the effect of temperature on an enzyme in this case peroxidase, by studying it decomposing hydrogen peroxide. Planning I am measuring the amount of gas given off in the reaction. By collecting this figure I can determine the rate of reaction. If I know the rate of reaction I can find out what temperature peroxidase works best at. Equipment · Trough · Clamp · 100ml measuring cylinder · Stop clock · 10ml measuring cylinder · Delivery tubes · Bung · Conical flask Variables Change
C. When the individual tests of these attributes are put together, the result is one overall test- the test of chivalry.
The Effect of Changing the Concentration of the Enzyme Catalyst on the Rate of Reaction on Hydrogen Peroxide
With this information we were able to identify any patterns and similarities. Hypothesis: The higher the temperature of water, potato and H²O², the rate at which the Enzyme will work will be faster therefore producing more oxygen. The reaction will be the same without the catalase (potato). Therefore in both experiments the Enzyme will work more rapidly and produce more oxygen. Aim: To test the hypothesis.
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
Temperature's Effect on the Production of Oxygen From Yeast and Hydrogen Peroxide Planning Aim --- My aim is to discover how temperature effects the production of oxygen from yeast and hydrogen peroxide. I will mix yeast with hydrogen peroxide and use an upturned measuring cylinder to measure how much oxygen is produced. To find out how much hydrogen peroxide and yeast I will use, I will carry out pre-tests. This will also aid accuracy of the final experiment by uncovering potential flaws in the method. Hypothesis and Theory
...ant to avoid fillers, preservatives, and artificial ingredients. A great idea for a healthy training treat is small bits of dried sweet potato.
we cant just look at one variable if we want it to be a fair test
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
The tennis ball is the constant variable factor (the variable that is kept the same, to make the investigation valid). The ball will dropped from increasing heights (cm-25, 50, 75,100,125,150,175,200) and the bounce of the ball will be measured. A sample size of 3 results will be taken from each height the ball is dropped. The same investigation will then be repeated, but one of the independent variables will be changed.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells
The pH of the solution would alter the rate of the reaction if it was
Investigating the Effect of the Enzyme Catalyse On Hydrogen Peroxide Introduction The aim of this experiment is to determine the effects of varying enzyme (catalyse) on Hydrogen Peroxide. Hydrogen Peroxide + Catalyse à Water + Oxygen 2H2O2 à H2O + O2 + Heat Apparatus & Diagram [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE] Bung Potato Hydrogen Peroxide Water Collected Oxygen Delivery Tube Measuring Cylinder [IMAGE] Using the Equipment Safely It is important that we use the apparatus carefully, as safety will be an issue throughout the whole experiment. We will wear goggles and an apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful, ensuring they don't come into contact with our eyes, skin or clothes. Catalyse is an enzyme found in all living cells.
Energy is essential for normal body functioning processes thus it is generated from glucose metabolism in an enzymatic chemical reactions. This yields energy as well as carbon dioxide after utilizing the glucose and oxygen. This cellular respiration is usually affected by various factors such as availability of oxygen without which anaerobic respiration takes place, availability of water, protoplasmic environment, temperature and carbon dioxide concentration.