The Effect Of Catalase On Hydrogen Peroxide
Planning
Catalase is an enzyme found in the blood and in most living cells its
job is to speed up the decomposition of hydrogen peroxide into water
and oxygen.
2H2O2 + Catalase ¾¾¾¾¾¾¾® 2H2O + O2
The chemical compound hydrogen peroxide (H2O2) is a viscous liquid
that has strong oxidizing properties and is also used as a powerful
bleaching agent that has found use as a disinfectant and (in strong
concentrations) as an oxidizer.
Hydrogen peroxide tends to decompose exothermically into water and
oxygen gas. The rate of decomposition is dependent on the temperature
and concentration of the peroxide, as well as the presence of
impurities and stabilizers.
Hydrogen peroxide is constructed from fat and amino acids. Fats and
amino acids are broken down by oxidation within membrane-bound
organelles called peroxisomes, which are present in plant and animal
cells. Liver cells contain more peroxisomes than other cells, because
one of the roles of the liver is the metabolism of fats and amino
acids.
Catalase works under, like many other enzymes, works only under
specific conditions.
Such as:
* pH
* Temperature
* Enzyme Concentration
* Substrate Concentration
My aim for this experiment is to investigate which percentage catalase
should be used for the main investigation where I will investigate the
rate of reaction when the percentage catalase I will use from doing
this experiment is added to various concentrations of hydrogen
peroxide. I predict that the higher the percentage of catalase added
to the hydrogen peroxide solution the great...
... middle of paper ...
... not wash out the percentage of catalase / hydrogen
peroxide.
* When recording the time you may not press the start button in
time.
* You may not record the amount of gas produced every 10 seconds
exactly.
* You may not read the volume of gas produced correctly.
In conclusion I have found that the rate of reaction does increase
when the amount of solution is increased or the percentage of catalase
or hydrogen peroxide is increased.
I have found that catalase does break down hydrogen peroxide into
water and oxygen as gas was produced and the solution of hydrogen
peroxide and catalase stayed a liquid but to be sure the products
produced from the experiments were oxygen and water we would have to
carry out a further experiment such as the test for oxygen 'lighting a
glowing splint'.
The purpose of this study is to analyze the activity of the enzyme, catalase, through our understanding
In both solutions of catalase there is a steady increase in reaction relative to the hydrogen peroxide concentration as it increases. A significant jump is observed in the carrot catalase solution between .25% and .5% whereas the pinto bean catalase solution has a steady increase. Each solution doesn’t generate much more reaction to the next increment of hydrogen peroxide concentration, 1%. In general it stayed level. This continued to be a trend for the pinto bean catalase solution, plateauing through to the 6% concentration of hydrogen peroxide. This is known as the point of saturation.
The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
A rate is a measure of change that occurs in a given time whilst a
This enzyme speeds up the break down of hydrogen peroxide into water and oxygen, as enzymes are biological catalysts. [IMAGE]The reaction: Hydrogen peroxide Water + Oxygen Catalase -------- [IMAGE] 2H2O2 2H2O + O2 Apparatus: Hydrogen Peroxide, Several sticks of celery, Stand, boss and clamp, 100ml conical flask, 25cm3 burette, 1800cm3 beaker, Rubber bung with delivery tube, Distilled water, Large container filled with water, 10cm3 measuring cylinder, 10cm3 syringe, 20cm3 syringe, Blender, Knife, Ceramic tile, Electronic balance (correct to 2 decimal places), Sieve, Stopwatch/timer. The variables: There are many possible variables in this investigation, such as pH, temperature, the concentration of substrate and the concentration of the enzyme.
== == == = This is what I'm going to be changing in the experiment and this will be the temperature and the concentration of the yeast. There are several variables in this experiment, they are: · Amount Used - Too much or too little of the hydrogen peroxide causes the reaction to speed up/slow down producing different amounts of oxygen.
2H2O(aq) à 2H2O(l) + O2 (g) It is able to speed up the decomposition of hydrogen peroxide because the shape of its active site matches the shape of the hydrogen peroxide molecule. This type of reaction were a molecule is broken down into smaller pieces is called an anabolic reaction. I will be studying the effect of concentration of the catalase in this reaction. Hypothesis Hydrogen peroxide will breakdown to oxygen in water in the presence of catalase.
The Effect of Surface Area on the Rate of Reaction Between Catalase from a Potato and Hydrogen Peroxide
In this experiment the enzyme peroxidase and the substrate hydrogen peroxide were not mixed initially, instead they were both placed in separate tubes and were incubated at a specific temperature, to prevent hydrogen peroxide from undergoing any reaction with peroxidase until they both acquire the required temperature.
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
The first experiments investigate the order of reaction with respect to the reactants; hydrogen peroxide, potassium iodide and sulphuric acid by varying the concentrations and plotting them against 1/time. An initial rate technique is used in this experiment so ‘the rate of reaction is inversely proportional to time.’ To find the order of reaction in respect to the reactants, 1/time is plotted against the concentration of Hydrogen Peroxide using the equation:
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells Introduction Enzymes are made of proteins and they speed up reactions, this means that they act as catalysts. Hydrogen peroxide is a byproduct of our cell's activities and is very toxic. The enzymes in our bodies break down the hydrogen peroxide at certain temperatures they work best at body temperature, which is approximately 37 degrees. At high temperatures, the cells begin to denature. This means that the hydrogen peroxide is prevented from being broken down because they will not 'fit' into the enzyme.[IMAGE] Objective I am going to find out how the concentration of the substrate, hydrogen peroxide affects the reaction in the catalase inside the potato cells.
Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide