Rubisco Case Study

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Name: Lambert Ntashamaje
Word count: 1734 words including intext citations and diagrams
Assignment: Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase (Rubisco)
Rubisco is a biological catalyst which fix carbon dioxide on Ribulose 1,5-bisphosphate(RuBP) in the process of photosynthesis. Rubisco is found in the stroma of chloroplast and cytoplasm of photosynthetic bacteria (Keys, 1986). The availability of Rubisco in both plants and archaea-bacteria makes rubisco the most plentiful protein in the world (Helmenstine, 2016). Rubisco’s active site is specific to RuBP, carbon dioxide and oxygen. Rubisco is called carboxylase/oxygenase because it can either catalyze carboxylation (carbon fixation) or oxygenation (oxygen fixation) to a 5-carbon molecule(5C) of RuBP during light independent reaction of photosynthesis (Keys, 1986).

There are four “forms” of Rubisco found on the …show more content…

Rubisco appears in the stroma of chloroplast in its inactivated form. According to Salvucci and Crafts‐Brandner (2004), Rubisco is activated and regulated by Rubisco activase (RCA). Rubisco activase is classified as AAA+( ATPase associated with variety of cellular activity) (Neuwald et al., 1999), and is mostly found on the chloroplast stroma. This activase enzyme activates Rubisco which later catalyzes the carboxylation of RuBP; furthermore, this carbon dioxide fixation is the first photosynthetic CO2 assimilation (Keys, 1986). In the presence of ATP, RCA activates Rubisco by removing phosphate sugar on its active site which acts as Rubisco’s inhibitor (Tabita et al., 2008). The activation of Rubisco is endergonic because it requires energy in the form of

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