The objective of our project was to determine if different wavelengths of light would affect the growth of the Rhodococcus genus and whether the two bacteria would handle DNA damage from UV light exposure differently. Our hypothesis is that various wavelengths of light would inhibit the growth of bacterial colonies of both Rhodococcus species differently. Also, we believe that Rhodococcus fascians (yellow) is more resistant to DNA damage from UV light than Rhodococcus corynebacterioides (red). To perform this experiment, we first made the bacterial growth medium was made. Then, the process of the dilution of the bacteria is performed to allow the bacteria to be seen easily. The diluted bacteria is spread upon the medium in the petri dishes
Ryazantseva, I. N., Saakov, V. S., Andreyeva, I. N., Ogorodnikova, T. I. and Zuev, Y. F. 2012. Response of pigmented Serratia marcescens to the illumination. Journal of Photochemistry and Photobiology B: Biology, 106 p. 18-23
al. (1994) explain that a complementary DNA for GFP produces a fluorescent product when expressed in E. coli cells as the expression of GFP can be used to monitor gene expression and protein localization in living things. In this experiment, the heat shock method will be used to deliver a vector (plasmid) of GFP to transform and grow E. coli bacteria. Four plates containing Luria Bertani (LB) broth and either –pGLO or +pGLO will have E. coli bacteria added to it. The plate containing –pGLO (no pGLO) and LB will show growth as ampicillin will be present killing bacteria but no glowing because no arabinose will be present for glowing to be activated, the same result will be seen in the plate containing +pGLO, LB and ampicillin.
Abstract: Bacterial transformation involves the change of genetic composition of bacteria by altering its genetic identity. The pGLO plasmid was ingrained in the E. coli cell, which allows the modified E. coli cell to begin to code for the GFP protein gene and the beta lactamase gene (ampicillin resistance gene). After modifying the bacteria cell, the changes involved with the plasmid were tested on 4 plates, two plates containing the pGLO plasmid (+) were treated with LB nutrient media. One of the LB plates contained arabinose in it, which should fluoresce green under UV light. The other LB plate contained ampicillin. Two other plates which did not contain the pGLO plasmid (-) both had LB media growth, one plate just to show cell growth,
Theodor’s experiment was created in part to learn which wavelengths (colors) of light were most effective in carrying out photosynthesis and to prove that it occurs in chloroplast. The experiment was inspired by Theodor’s observation of aerobic bacteria. Theodor observed that aerobic bacteria would move towards the chloroplasts of green algae. Theodor hypothesized that the reason why the bacteria moved toward the chloroplasts was because the organelle generated oxygen via photosynthesis. If photosynthesis occurs in chloroplasts, then the bacteria would aggregate on the chloroplasts producing the most oxygen. Theodor’s experiment was essential because it demonstrated that chloroplasts were the site of photosynthesis. Furthermore,
It can be concluded that chloroplast is responsible for photosynthesis, with blue light forming the highest rate of photosynthetic activity. The widespread use of coloured netting in the future could result in indoor plant growth that is unreliant on weather, and the ease of the production of crops with the desirable phenotypes. However, future research is still required to eradicate any unknown data and determine plant responses in relation to wavelength
Moreover, a future experiment is to determine the effect that the distance between the lamp and the solution has on the rate of photosynthesis. Several experiments with a similar setup to this experiment that vary the distances between the lamp and solution could be used to test this.
[7] Rothschild, Lynn J. “The Influence of UV Radiation on Protistan Evolution.” Journal of Eukaryotic Microbiology (1999), Issue 46 no. 5 pg. 548-555.
Bacterial pigment plays role as antioxidant by inhibiting photodynamic lipid peroxidation in liposome and give protection against photodamage (Rajagopal et al. 1997).Xanthomonadin is example of this bacterial pigment.
Talaro , K., & Chess, B. (2012). Foundations in microbiology. (8th ed., pp. 563-564). New York, NY:
As a species, Cyanobacteria are chemically diverse, and owe their bluish colour to the pigment phycocyanin, which is used to capture light for photosynthesis. Because they are able to photosynthesize, they are able to...
In the case of temperatures the cultures were incubated at each determined temperature. For the UV radiation, cells were exposed to UV light for 10 seconds and then grown in 30oC. For the EtBr treatment, 50ul of EtBr was added to the growth medium and cells were incubated at 30oC. In the case of sunlight exposure, cells were exposed to sunlight directly and grown at room temperature
In this experiment, uvrA phr E. coli cells, repair-deficient mutant strain, were first exposed to one or two seconds or UV radiation. Since this strain lack both nucleotide excision repair and phtoreactivation to repair resulting pyrimidine dimers, mutations resulting from error-prone repair may occur in the lac operon. By plating out the mutated E. coli on MacConkey/lactose medium, we can determine whether the cells are lac operon mutants by assessing the colour of the colonies. MacConkey agar, acts as a pH indicator and stains bacteria that ferment lactose red (acidic pH). Bacteria that cannot use lactose will use the agar’s other constituent, peptone, instead, producing ammonia that gives a basic pH, and thus the colonies will appear pale.
Photosynthesis is a widely studied topic among the world of science due to its importance for life and its many uses. Photosynthetic pigments reflect and absorb different wavelengths of visible light based off their polarity. In this experiment, we studied photosynthetic pigments, first, by determining polarity and then, by measuring the amount of light of a given wavelength that a pigment absorbs. We used two methods in this experiment, chromatography and spectrophotometry. For the first portion of our experiment we determined the distance each pigment migrated, their R_f values, and their polarity. Our predications based off polarity, lead to our hypothesis
The purpose of the study is to identify an unknown microorganism using multiple microbiology lab techniques. Through this process I will gain knowledge on how to perform these techniques as well as the importance of these tests on identifying unknown microorganisms. This is significant as the goal of this course is to familiarize ourselves with the common microbiology tests as well as the microorganisms we encounter in our daily activities.
Leboffe, M. J., & Pierce, B. E. (2010). Microbiology: Laboratory Theory and Application, Third Edition 3rd Edition (3rd Ed.). Morton Publishing