For this experiment a standard curve and the absorbance values in two separate trials of the proteins powders Kroger whey &soy-protein vanilla and the Designer whey with acti-blend French vanilla were tested to determine which had a higher concentration the generic or a designer protein powder when analyzed in the same level of dilution. For the analysis and determination of the concentrations of the protein powders the standard curve needs to be created first. To create a standard curve, you will need to make six solutions from a stock solution with a 20mg/mL concentration, a calculated volume of water, and a volume of Buiret’s reagent. These were calculated using the formula C_1 V_1=C_2 V_2 these final numbers can be seen in figure one. Pipette the amount seen in below into the respective test tube and placing them individually into the spectrophotometer set at an absorbance of 565nm. …show more content…
Taking the absorbances that we observed we plotted the absorbance the (dependent in the experiment) and the concentration of protein (the independent in this experiment). Graphing this in excel gave the formula y=0.00118x-0.002 this formula will be used later to determine the concentration of our protein powders. When making the dilution of the protein powders we had initially had to calculate how many fluid oz. are in a serving for each protein powder we did this by using the conversion 1fluid oz. per 29.57mL (8fluid oz. x29.57mL/1 fluid oz.=236.56mL). For the next step of the calculations take the amount of powder in one serving size and divide it by the mL in a standard serving then multiple it by 2mL to give the number of grams of each protein powder in its respective 20 mL solution 18 of which are water. The calculations can be seen in the
Data from Table 1. confirms the theory that as the concentration of glucose increases so will the absorbance of the solution when examined with the glucose oxidase/horseradish peroxidase assay. Glucose within the context of this assay is determined by the amount of ferricyanide, determined by absornace, which is produced in a one to one ratio.1 Furthermore when examining the glucose standards, a linear calibration curve was able to be produced (shown as Figure 1). Noted the R2 value of the y = 1.808x - 0.0125 trend line is 0.9958, which is statistically considered linear. From this calibration curve the absorbance values of unknowns samples can be compared, and the correlated glucose concentration can then be approximated.
Forensic Science Introduction: Someone in a restaurant has suddenly fallen ill and a mystery powder has been discovered with the victim. As the chief investigator, your duty is to identify the mystery substance through a lab. In this lab, it will consist of five known compounds and one unknown compound. Your job is to distinguish which one out of the five substances is the mystery powder. To figure out the mystery matter you will have to compare their physical and chemical properties and match them with the appropriate compound.
Measurement of Ki of a competitive inhibitor (lactose)- For the third part of the experiment, three duplicates of 11 test tubes were set up exactly as in the previous step. The first duplicate was series A while the second duplicate was series B and the last one was series C. Series A did not contained any inhibitors so additional water was added to each tube. Series B contained the 50mM lactose while series C contained the 150mM lactose. The amounts of buffer, ONPG, and water added to each tube in each series are listed in detailed in tables 4, 6, and 8. The Lineweaver- Burk plot of each series was created using the same steps used in the second part of the experiment. Then, the obtained line equation from each plot was used to calculate
To test for protein, drops of biuret reagent and drops of each food were mixed in a test tube. If the mixture changed to a purple/pink/blue color, it indicated that the food contains protein. To test for starch, drops of iodine are mixed with
From the moment of its creation, protein powder has been engulfed in a tornado of controversy. Doctors, scientists, and athletes have debated on whether or not it should be used for years. Protein provides the building blocks for all cells and membranes, hormones and various growth factors. Elena Voropay, a writer for Iron Man Magazine, says “Protein is found literally everywhere in your system: there is protein in your muscle tissues, your heart, in the enzymes that digest your food, in your skin cells and even within your blood” (Voropay 1).
The combination of these plant proteins makes the amino acid of the product more balanced in nature. It is also free of gluten because they are not made from any kind of grain. To sweeten the product, Tart Cherry is made use of and Turmeric Extract is made use of to give it a high rate of antioxidant. To make the Turmeric Extract more effective, the extract of Black Pepper is equally made use of. The product is ideal for recovery of the muscles after a workout. The Probiotics are added to help in the process of digestion. This product can be said to be one of the best Vegan protein Powder according to this customer and can be rated with a 6 on a scale of 1 to 10. The con of this product according to the customer also is the cost of the
Start to prepare the sample in a 10mL (10000µm) place 0.4mL (400µm) of the stock Ferritin solution 0.5g/mL in 0.15M sodium acetate (AcONa). Afterwards add 0.8mL (800µm) at 2M of sulphuric acid (H₂SO₄) and 0.8mL (800µm) at 5mM of dihydroxyfumarate (C₄H₄O₆). Wait for 30 minutes then complement the solution with 1.6mL (1600µm) at 2.5M of sodium acetate (NaOAc) followed by 0.8mL (800µm) at 12.5mM ferrozine (C20H12N4Na2O6S2). Lastly wait another 30 minutes afterwards add distilled water using a stopper to complete the solution to 10mL (10000µm) then put the rubber stopper on the volumetric flask and mix till you are sure that all the contents are mixed. Again, set up the spectrometer to 562nm and using the 2 samples that were made take one from each to measure the absorbance plus a third one from either one of the solution.
Due to the nature of amino acids, a titration curve can be employed to identify
Protein is best when it is sourced from whole foods, but if you’re not able to get the entire daily protein requirement from food, you can get it from protein powders. Protein powders can serve as an alternate meal or as a post-workout supplement. Protein powders are convenient and can be easily absorbed and digested when compared to whole foods.
11.) Subtract the mass of the evaporating dish from the mass of the evaporating dish and it's contents. Multiply that number by 10 to get the solubilty in grams per 100 cm3 of water.
Introduction to Protein Synthesis In order to understand the methods in which a macrolide attacks a bacterium, it is important to understand the structure and function of the bacterial mechanism for protein synthesis. A bacterium’s DNA holds the structure of a circular double strand. Like the DNA of any other living organism, it contains the genetic coding for the bacteria. This genetic coding is crucial for the growth, development, and survival of the cell.
The Muscle Milk Pro Series Protein Shake is fuel that provides a bodybuilder with essential nutrients required to recover after intensive training sessions. It can also be used before a workout, or as a high protein snack at any time of the day or night. It aids in strength, long lasting energy, and satisfies hunger. While it is recommended that healthy people rely primarily on consumption of whole foods to meet their nutritional needs, this product is an excellent choice for those times when it is not possible to have a full meal, or for recovery from workouts between meal times. With a multitude of flavor options, there is something to suit the tastes of everyone. Athletes in both high school and college rely on Muscle Milk Pro Series Protein Shakes to ensure proper muscle building and post workout recovery of their athletes. This is a safe and highly regarded
This poster presents information about the function of protein and their structures. It provides a brief explanation of recommendation of protein intake, and illustrate how excessive of protein intake or protein deficiency can lead to health problems. This paper will also display the sources of protein. Finally, this paper will point out the essential of protein how it important for body. Introduction: Proteins are found throughout the body and over 40 percent of body protein found in skeletal muscle, over 25% found in body organs and the rest found mostly in the skin and blood.
100)/5=2.5% (0.05cm3 x 100)/15=7.55% (0.05cm3 x 100)/2=1% (0.05cm3 x 100)/50=25% Measuring beaker (0.2 cm3 x 100)/250 =0.08% Measuring tube (0.1 cm3 x 100)/10 =1% Measuring tube containing hydrogen peroxide (0.1cm3 x 100)/50 =0.2% Measuring tube containing water (0.1cm3 x 100)/100 =0.1% Measuring tube to calculate volume of beads (1cm3 x 100)/8 =12.5%
The main target market of All Max Whey Protein is people of the age group of 18 and above. Contrary to the popular belief that only body builders and athletes consume whey protein, children, men, women or anyone looking for a simple way to increase protein in their diet can benefit from it .This whey brand is ‘comparatively’ easier on the pocket and caters to people belonging to all classes of the society as a 2.2 pound tub cost around 1500 Rs which is at least 12% cheaper than the other competing brands in the market.