Photosynthesis Lab Report

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Photosynthesis is one of the most important biological processes in life. In a very broad view, it takes carbon dioxide and converts it to oxygen to release in the environment. Photosynthesis goes through two stages: photochemical and biochemical. Photochemical reactions are dependent on light and capture energy. The objective of the experiment is to observe the rate of photochemical reactions with different light intensities and light wavelengths. In order to conduct this experiment, we determined the absorbance of freshly extracted chloroplasts at different light treatments and times. The different light treatments used in this experiment was foil wrapped (dark), 24 cm distance from light, 30 cm distance from light, and 49 cm distance from light. The independent variable in this experiment is the different light treatments used, and the dependent variable in the experiment is the different absorbance values recorded by the spectrophotometer. The null hypothesis constructed in the experiment is that the distance of the chloroplasts solutions will have no effect on the absorbance. …show more content…

One factor that effects photosynthesis is the pigment. Photosynthetic pigments determine the wavelengths of light that are absorbed. Therefore, pigments have an effect on photosynthesis. Each pigment has a different light absorption. The objective of this experiment is the observe and understand the effect of pigments on photosynthesis by finding the absorption value of each pigment at different wavelengths. The pigments that were used in the experiment were carotene, chlorophyll a, chlorophyll b, and xanthophyll. The wavelengths used in the experiment were 400 nm, 450nm, 500nm, 550nm, 600nm, 650nm, and 700nm.The independent variable is the pigment solutions, and the dependent variable is the absorption value. The null hypothesis is that the pigments have no effect on the absorption value. The results are shown below in the

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