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Patient safety in hospital setting
Patient safety in hospital setting
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INTRODUCTION
Nature of the Bacteria
Mycobacterium tuberculosis (M. tuberculosis) is the bacterium that causes the disease tuberculosis (TB). A distinctive characteristic of the genus Mycobacteria is the presence of a thick lipid-rich cell wall and resistance to the decolourization step of the gram stain (being acid-fast). The acid-fast characteristic of the M. tuberculosis is the result of a waxy, lipid-rich cell wall. The cell envelope of the tubercle bacilli contains a layer beyond the peptidoglycan which is exceptionally rich in lipids, glycolipids and polysaccharides. The bacterium is gram positive bacillus which is an obligate aerobe, is non-motile, a non-endospore forming and is non-capsulated. The microscopic appearance of M. tuberculosis is seen as straight, slightly curved rods approximately 3 x 0.3µm in size. In liquid culture media, the bacteria usually grow as twisted rope-like pellets known as ‘serpentine cords’. M. tuberculosis is capable of growing on a wide range of enriched culture media such as Lowenstein-Jensen medium or Middlebrook medium. The optimum growth temperature of the pathogenic organsim is 35-37°C and unlike most other mycobacteria, it cannot grow at a temperature of 25°C or 41°C. M. tuberculosis is an airborne pathogen that is transmitted from person to person, usually infecting the respiratory tract through inhalation (Greenwood, et al., 2012).
Other key features of M. tuberculosis are its slow growth rate, dormancy, intracellular pathogenesis and genetic homogeneity. In infected animals or synthetic medium, M. tuberculosis has a generation time of about 24 hours. The short generation time contributes to the chronic nature of the tuberculosis disease and long treatment courses for infected patien...
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...ST. A person could show positive for LTBI, when in fact it is the vaccine causing the positive result. There are conflicting policies on the BCG vaccination both nationally and internationally due to the lack of effectiveness and potential loss of the TST (HSE/HSPC, 2010).
Healthcare workers working in close proximity with infected patients are required to take various standard precautions. These standard precautions include hand hygiene, wearing personal protective equipment, appropriate management of sharps and waste and appropriate decontamination of the working environment. For their own protection, a healthcare worker must assume that all blood, body fluids and secretions from patients are potentially hazardous and my cause infection. If the appropriate precautions are taken, healthcare workers can prevent becoming infected with TB themselves (HSE/HSPC, 2010).
In this lab project, the microbiology students were given 2 unknown bacteria in a mixed broth each broth being numbered. The goal of this project is to determine the species of bacteria in the broth. They had to separate and isolate the bacteria from the mixed broth and ran numerous tests to identify the unknown bacteria. The significance of identifying an unknown bacteria is in a clinical setting. Determining the exact bacteria in order to prescribe the right treatment for the patient. This project is significant for a microbiology students because it gives necessary skills to them for future careers relating to clinical and research work.
The purpose of this laboratory is to learn about cultural, morphological, and biochemical characteristics that are used in identifying bacterial isolates. Besides identifying the unknown culture, students also gain an understanding of the process of identification and the techniques and theory behind the process. Experiments such as gram stain, negative stain, endospore and other important tests in identifying unknown bacteria are performed. Various chemical tests were done and the results were carefully determined to identify the unknown bacteria. First session of lab started of by the selection of an unknown bacterium then inoculations of 2 tryptic soy gar (TSA) slants, 1 nutrient broth (TSB), 1 nutrient gelatin deep, 1 motility
The lack of information, other the endangering other patients that sought for medial care, also put at risk the heath of doctors, nurses and other medical assistants. Used to treating infectious diseases without the proper protection equipment, such as gloves, goggles, gown and masks, the medical staff treated Tse, and other patients infected with SARS, without any precaution. The mentioned careless procedure contributed for the infection of many medical professionals, and the consequent spread of the disease in Toronto.
An estimated one third of the world 's population is infected with Mycobacterium tuberculosis. In 2012 there were 9,975 cases of TB reported and of those cases 28% where Hispanic or Latino. This respiratory disease is the most common in the lungs (World Health Organization, 2014). The brain which causes tuberculosis meningitis, genitourinary TB, gastrointestinal TB, tuberculosis lymphadenitis, cutaneous TB, uterus ovarian TB and Osteo- articular skeletal bone and joints are also parts of the body that are affected (Article base, 2008). Symptoms of TB are chills, fever, night sweats, hemoptysis, cough lasting more than 3 weeks, chest pains, and weight loss (Tuberculosis, 2014). Transmission of TB is through a sneeze, a cough, speaking, or singing in which the person has the
Tuberculosis as (TB) is caused by Mycobacterium tuberculosis. It is slow growing bacteria that thrive in areas of the body that are rich in blood and oxygen, such as the lungs. Tuberculosis develops when Mycobacterium tuberculosis bacteria are inhaled into the lungs. The infection usually stays in the lungs, but the bacteria can travel through the bloodstream to other parts of the body.
TB germs enter the body, the immune system builds a wall around them. While TB
"Preventing Exposures to Bloodborne Pathogens among Paramedics." Workplace Solutions (2010): n. pag. National Institute for Occupational Safety and Health. Web. 9 Feb. 2014.
Safety is focused on reducing the chance of harm to staff and patients. The 2016 National Patient Safety Goals for Hospitals includes criteria such as using two forms of identification when caring for a patient to ensure the right patient is being treated, proper hand washing techniques to prevent nosocomial infections and reporting critical information promptly (Joint Commission, 2015). It is important that nurses follow standards and protocols intending to patients to decrease adverse
"TUBERCULOSIS DIAGNOSTICS Xpert MTB/RIF Test." . World Health Organization, n.d. Web. 13 May 2014. .
...n of purified protein derivative or PPD, which is derived from cultures of M tuberculosis (Nayak & Acharjya, 2012). According to Nayak and Acharjya (2012), a person who has been exposed to the bacteria is expected to mount an immune response in the skin containing the bacterial proteins. A positive skin test means an infection with M tuberculosis has occurred, but does not prove tuberculosis disease. However, false positive results can occur with exposure to, or disease of, nontuberculous mycobacteria (Alguire, 2009). In addition, the causes of false positive TB skin test include previous BCG vaccination, incorrect method of skin test administration, incorrect interpretation of reactions, or incorrect bottle of antigen used (Nayak & Acharjya, 2012). Due to the test’s low specificity, most positive reactions in low-risk individuals are false positive (Starke, 1996).
Mycobacterium tuberculosis is a nonmotile, acid-fast, obligate aerobe. The bacilli are 2-4 um in length and have a very slow generation time of between 15 and 20 hours. The cell wall of the mycobacterium is unique in that it is composed mainly of acidic waxes, specifically mycolic acids. M. tuberculosis is unusually resistant to drying and chemicals, contributing to the ease with which it is transmitted.
Bacterial cells, like plant cells, are surrounded by a cell wall. However, bacterial cell walls are made up of polysaccharide chains linked to amino acids, while plant cell walls are made up of cellulose, which contains no amino acids. Many bacteria secrete a slimy capsule around the outside of the cell wall. The capsule provides additional protection for the cell. Many of the bacteria that cause diseases in animals are surrounded by a capsule. The capsule prevents the white blood cells and antibodies from destroying the invading bacterium. Inside the capsule and the cell wall is the cell membrane. In aerobic bacteria, the reactions of cellular respiration take place on fingerlike infoldings of the cell membrane. Ribosomes are scattered throughout the cytoplasm, and the DNA is generally found in the center of the cell. Many bacilli and spirilla have flagella, which are used for locomotion in water. A few types of bacteria that lack flagella move by gliding on a surface. However, the mechanism of this gliding motion is unknown. Most bacteria are aerobic, they require free oxygen to carry on cellular respiration. Some bacteria, called facultatibe anaerobes can live in either the presence or absence of free oxygen. They obtain energy either by aerobic respiration when oxygen is present or by fermentation when oxygen is absent. Still other bacteria cannot live in the presence of oxygen. These are called obligate anaerobes. Such bacteria obtain energy only fermentation. Through fermentation, different groups of bacteria produce a wide variety of organic compounds. Besides ethyl alcohol and lactic acid, bacterial fermentation can produce acetic acid, acetone, butyl alcohol, glycol, butyric acid, propionic acid, and methane, the main component of natural gas. Most bacteria are heterotrophic bacteria are either saprophytes or parasites. Saprophytes feed on the remains of dead plants and animals, and ordinarily do not cause disease. They release digestive enzymes onto the organic matter. The enzymes breakdown the large food molecules into smaller molecules, which are absorbed by the bacterial cells. Parasites live on or in living organisms, and may cause disease. A few types of bacteria are Autotrophic, they can synthesize the organic nutrients they require from inorganic substances. Autotrophic bacteria are either photosynthetic or Chemosynthetic. The photosynthetic bacteria contain chlorophyll that are different from the plant chlorophyll. In bacterial photosynthesis, hydrogen is obtained by the splitting of compounds other than water.
Tuberculosis is an infection caused by Mycobacterium Tuberculosis, an acid-fast Gram-positive bacillus, and “is characterized by progressive necrosis of the lung tissue” (Tamaro & Lewis, 2005). Tuberculosis is caused by many debilitating conditions like immunosuppression and chronic lung disease, among others. Nevertheless, human immunodeficiency virus (HIV), the virus that causes acquired immune deficiency syndrome (AIDS), remains the leading cause of tuberculosis worldwide. Tuberculosis can present in one of two types: active tuberculosis and latent tuberculosis. Prompt treatment prevents latent tuberculosis from evolving into active tuberculosis. (“Basic TB Facts,” 2012).
...s and hoses, control buttons, switches, hand pieces, and X-ray units (Collins). After every patient’s visit, the operatory is to be sterilized and disinfected. All areas that were that were not covered with a barrier, or if the barrier was compromised, must be wiped down with surface disinfectants similarly used in hospitals. OSHA requires disinfectants to be potent enough to fight against HIV and HBV infections (Collins). If there is any blood present on a surface, tuberculocidal type disinfectant should be utilized.
Avoiding infection or, at least, breaking the chain of transmission is vital in any setting, but more so in healthcare environments where infections and vulnerable hosts are moving under the same roof. What needs to be done, then?