Muscles Lab Report

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Introduction
Force-length curves offer insight into how muscles function. Muscles are made up of fascicles, which are bundles of muscle fibers. Muscle fibers are allowed to shorten during a contraction when myosin moves past actin in sarcomeres. The length of the sarcomeres affects the number of action-myosin cross-bridges, therefore affecting the force output of the muscle. When a sarcomere is very short, the myosin cannot move (as it essentially hits a brick wall), so force output is very low. Additionally, when a sarcomere is very long, there are little-to-no actin-myosin cross-bridges and the force output remains very low. However, when the sarcomere length is somewhere in the middle, the number of actin-myosin cross-bridges increases …show more content…

Then, all subjects were plotted on a shared graph. One subject’s protocol was different and had different angles. This data had to be excluded from further analysis due to this. OpenSim was utilized to find the moment arm and muscle lengths at each angle. Rectus femoris and biceps femoris long head were chosen as representative muscles for extension and flexion, respectively. Using this new information, muscle force could be calculated. Force-length curves and scaled force-length curves were formed for the quadriceps and the hamstrings. To provide statistical information, the average force was plotted with error bars to show the standard deviation of each collection …show more content…

The slope and shape looks like an accepted force-length curve; however, it is upside down. This could be due to OpenSim’s coordinate system, because it gave negative moment arms for those muscles, but the Biodex software did not give negative torques. Perhaps if I chose a different representative muscle, instead of the biceps femoris long head, I would have gotten a better plot. The error bars on this plot were smaller. At a muscle length between 0.39 and 0.4 meters, they reach a minimum. This suggests that torque output during flexion is more uniform between subjects, which contradicts the earlier notion that torque output is varied between subjects. Therefore, the quadriceps are varied between subjects whereas the hamstrings aren’t as varied between

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