Investigation to Determine the Relationship Between Surface Area and Rate of Oxygen Production

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Investigation to Determine the Relationship Between Surface Area and Rate of Oxygen Production

To determine the relationship between surface area and rate of oxygen

production when a potato is placed in hydrogen peroxide (H2O2)

solution.

Background Information

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Potato contains enzymes such as catalase. Catalase are protein

molecules, which are found in animal and plant cells. Catalase speeds

up specific reactions in the cells. They all have very specific duty,

as each enzyme just performs one particular reaction.

It is able to speed up the decomposition of Hydrogen Peroxide because

the shape of its active site matches the shape of the Hydrogen

Peroxide molecule. This type of reaction where a molecule is broken

down into smaller pieces is called an anabolic reaction.

Variables

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In my investigation I will have two types of variables:-

a) Independent variables are variables that I can change. The

independent variable for my investigation is:

Surface area – the more surface area the more chance of collisions and

therefore a quicker reaction.

b) Dependent variable is the variable that is measured. In my

investigation the dependent variable is:

Volume of oxygen gas produced over a period of one minute

Variables that I kept constant are:

Temperature – the rate of reaction depends on the temperature as

enzymes work best at certain temperatures.

Concentration of Catalase (enzyme) – the more concentrated the quicker

the reaction.

Concentration of Hydrogen Peroxide – the more concentrated the quicker

the reaction.

FAIR TEST

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I will make my investigation a fair test by controlling variables:

I will make sure that no air gets into the measuring cylinder until

the experiment has started. I will keep the surface area of the potato

the same. I will control the Surface area of the potato by using a

ruler to measure out how much to cut and by using a knife to cut

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