Horseradish Peroxidase Experiment

513 Words2 Pages

The purpose of conducting an experiment using various temperatures, pH levels, and enzyme concentrations was to observe the effects these factors had on the rate of horseradish peroxidase activity. Furthermore, testing how peroxidase reacts to different set ambient conditions and finding the conditions that allow for maximum rates. Enzymes are used in this experiment to demonstrate the importance of certain environmental conditions since they affect the speed of an enzymatic reaction in order to carry out life. An extract from horseradish is used as the enzyme since it contains peroxidase which has a highly accessible active site. The experiment was administered by using a spectrometer as a tool to measure oxygen production using an indicator called guaiacol which would allow the rate of horseradish peroxidase activity to be seen. Environmental circumstances, such as enzyme concentration, temperature, and pH, are tested by using guaiacol as an indicator of oxygen production and H2O2 as an oxygen producing agent. The ending results conclude that higher enzyme concentration can increase the speed of the rate of enzyme activity. The results also conclude …show more content…

Enzymes are used to carry out reactions in a rapid manner otherwise the reaction would occur very slowly thus not being able to sustain life. Enzymes bind to a substrate that is specific to their task and then conforms into a product that is needed; the enzyme is then able to catalyze more of the same reaction. Enzymes and substrates act as a lock and a key since enzymes are made for a specific substrate and is able to form an enzyme-substrate complex (Department of Biology). Thus changes of the shape of an enzyme can inhibit its ability to catalyze a reaction. If the enzyme shape is alternated due to environmental conditions, it is denatured and can no longer act as a catalyst. Peroxidase is the type of enzyme used in this

Show More
Related

  • Horseradish Peroxidase Lab Report

    1394 Words  | 3 Pages

    This yellow species can then be measured using UV absorbance (max abs = 420 nm), and thus the concentration of the can species determined.1 Horseradish peroxidase in important in the glucose assay because it catalyzes a reaction that includes one of the products from the glucose oxidase reaction, H2O2. There will be one H2O2 produced for every oxidized B-D-glucose, which will then be used to oxidize one ferrocyanide into the one measurable ferricyanide. Therefore, using the enzymes glucose oxidase and horseradish peroxidase in a consecutive manner, users can determine the concentration of glucose present in solution by simply measuring the amount of ferricyanide produced because of it (this is a one to one ratio).

    Read More

  • Substrate Concentration Lab Report

    592 Words  | 2 Pages

    However, at 3% substrate concentration, the hydrogen peroxide decomposition showed an immediate peak of up to 3.8 mm in height. As the substrate concentration slowly increased, enzyme

    Read More

  • Enzyme Formal Lab Report

    1157 Words  | 3 Pages

    Living organisms undergo chemical reactions with the help of unique proteins known as enzymes. Enzymes significantly assist in these processes by accelerating the rate of reaction in order to maintain life in the organism. Without enzymes, an organism would not be able to survive as long, because its chemical reactions would be too slow to prolong life. The properties and functions of enzymes during chemical reactions can help analyze the activity of the specific enzyme catalase, which can be found in bovine liver and yeast. Our hypothesis regarding enzyme activity is that the aspects of biology and environmental factors contribute to the different enzyme activities between bovine liver and yeast.

    Read More

  • Pinto Beans Essay

    1230 Words  | 3 Pages

    Catalase is a common enzyme that is produced in all living organisms. All living organisms are made up of cells and within the cells, enzymes function to increase the rate of chemical reactions. Enzymes function to create the same reactions using a lower amount of energy. The reactions of catalase play an important role to life, for example, it breaks down hydrogen peroxide into oxygen and water. Our group developed an experiment to test the rate of reaction of catalase in whole carrots and pinto beans with various concentrations of hydrogen peroxide. Almost all enzymes are proteins and proteins are made up of amino acids. The areas within an enzyme speed up the chemical reactions which are known as the active sites, and are also where the

    Read More

  • The Effect of Temperature on the Activity of Rennin in Milk

    3329 Words  | 7 Pages

    When this substrate fits into the active site, it forms an enzyme-substrate complex. This means that an enzyme is specific. The bonds that hold enzymes together are quite weak and so are easily broken by conditions that are very different when compared with their optimum conditions. When these bonds are broken the enzyme, along with the active site, is deformed, thus deactivating the enzyme. This is known as a denatured enzyme.

    Read More

  • The Effect of Temperature on the Rate of Respiration in Yeast

    1871 Words  | 4 Pages

    Catalysis occurs because substance S fits precisely into surface of the enzyme E, so this reaction and no others are speeded up. Diagram showing an enzyme catalsying the breakdown of its substrate into two product molecules. As can be seen from the diagram, if the enzyme changes shape, the active site (the area where the substrate reacts) would no longer be able to fit the substrate. This would mean the enzyme would lose its effect; the substrate would not break down.

    Read More

  • The Effect of Temperature on the Activity of the Enzyme Catalase

    1594 Words  | 4 Pages

    The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.

    Read More

  • Investigating the Effect of Substrate Concentration on Catalase Reaction

    4867 Words  | 10 Pages

    Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).

    Read More

  • Catalase Reaction Essay

    1322 Words  | 3 Pages

    Enzymes are biological catalysts - catalysts are substances that increase the rate of chemical reactions without being altered itself. Enzymes are also proteins that fold into complex shapes that allow smaller molecules to fit into them. The place where these substrate molecules fit is called the active site. The active site is the region of an enzyme where substrate molecules bind and undergo a chemical reaction. The active site consists of residues that form temporary bonds with the substrate and residues that catalyse a reaction of that substrate. (Clark, 2016)

    Read More

  • Investigating the Effect of Enzyme Concentration on the Hydrolysis of Starch with Amylase

    4181 Words  | 9 Pages

    Enzymes have the ability to act on a small group of chemically similar substances. Enzymes are very specific, in the sense that each enzyme is limited to interact with only one set of reactants; the reactants are referred to as substrates. Substrates of an enzyme are the chemicals altered by enzyme-catalysed reactions. The extreme specific nature of enzymes are because of the complicated three-dimensional shape, which is due to the particular way the amino acid chain of proteins folds.

    Read More

  • The Effect of Surface Area on the Rate of Reaction Between Catalase from a Potato and Hydrogen Peroxide

    734 Words  | 2 Pages

    The Effect of Surface Area on the Rate of Reaction Between Catalase from a Potato and Hydrogen Peroxide

    Read More

  • Enzyme Peroxidase Lab Report

    454 Words  | 1 Pages

    The results of this experiment showed a specific pattern. As the temperature increased, the absorbance recorded by the spectrophotometer increased indicating that the activity of peroxidase enzyme has increased.At 4C the absorbance was low indicating a low peroxidase activity or reaction rate. At 23C the absorbance increased indicating an increase in peroxidase activity. At 32C the absorbance reached its maximum indicating that peroxidase activity reached its highest value and so 32 C could be considered as the optimum temperature of peroxidase enzyme. Yet as the temperature increased up to 60C, the absorbance decreased greatly indicating that peroxidase activity has decreased. This happened because at low temperature such as 4 C the kinetic energy of both enzyme and substrate molecules was low so they moved very slowly, collided less frequently and formed less enzyme-substrate complexes and so little or no products. Yet, at 23 C, as the temperature increased, enzyme and substrate molecules

    Read More

  • Lab: Enzymes – Protein Catalysts

    870 Words  | 2 Pages

    Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.

    Read More

  • Lactase Enzyme Lab Report

    1772 Words  | 4 Pages

    Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is important that a specific enzyme is present during the process. For example, lactase must be able to collaborate with lactose in order to break it down (Madar & Windelspecht, 105).

    Read More

  • The Importance Of Enzymes

    1073 Words  | 3 Pages

    Researchers then hypothesized that the results would indicate the greatest amount of potato enzyme activity level will take place at room temperature. In this experiment, researchers used potato extract and different temperature levels to test the hypothesis. Moreover, researchers wanted to test the color intensity scale and how specific catechol oxidase is for catechol. In this experiment, researchers used dH2O, catechol solution, hydroquinone, and potato extract. Lastly, researchers tested the substrate concentration and how it has an effect on enzyme activity.

    Read More

More about Horseradish Peroxidase Experiment

Open Document