Decomposition of Hydrogen Peroxide
Aim: My aim in this experiment is to investigate the effect of
changing the concentration of the substance (Hydrogen Peroxide) in an
enzyme controlled reaction.
Prediction: I predict that the higher the amount of Hydrogen Peroxide
I use then the faster the displacement reaction will be. Therefore,
the less substrate I use then the slower the displacement reaction
will be.
Hypothesis: I performed a preliminary experiment to distinguish the
amount of yeast I will use for the actual experiment.
0.5 grams
1.0 gram
1.5 gram
2.0 grams
2.5 grams
30
72
94
140
202
This therefore shows me that the amount of yeast is directly
proportional to the amount of bubbles.
Apparatus:
In order to complete this experiment I will need the following
apparatus
--------------------------------------------------------------
· Measuring beaker
· Measuring cylinder
· Side arm test tube & cork
· Rubber bung
· Large syringe
· Small syringe
· Metal rod
· Metal scoop
· Electronic scales
· Stop watch
· Goggles
· Hydrogen Peroxide
· Water
· Yeast
Method: To begin this experiment I set up the apparatus like its shown
in the diagram. I got my two syringes and filled each with a different
solution. I filled one of the syringes with one millimetre of yeast
solution and the other with ten millimetres of hydrogen peroxide. I
then squirted each substance into the side arm test tube to form the
reaction. I then waited to see how much oxygen would fill the
measuring cylinder. After three minutes I measured to see how much
oxygen had passed into the cylinder. This first test was done with
100% hydrogen peroxide and no water. This time I was going to repeat
the same experiment but with different amounts of solution. I made
this test 80% hydrogen peroxide and 20% water. I took the results and
repeated it again with 60% hydrogen peroxide and 40% water. I now made
it 40% hydrogen peroxide and 60% water. Then I made it 20% hydrogen
peroxide and 80% water. After doing this and collecting the results I
had to repeat the whole procedure twice more so I could then get an
average results table. This made sure that I got an average and not
just one set of results. This makes it a fair test as well as keeping
all measurement roughly the same and room temperature kept the same
throughout the entire experiment. I would also need to keep this
experiment safe by wearing goggles to protect my eyes.
Diagram:
[IMAGE]
Results:
1st Results
% Of H2O2
Volume of H2O2 in ml
Volume of H2O in ml
Minutes
=======
Seconds
=======
100%
10
0
1.36
96
80%
8
2
1.57
117
60%
6
4
2.25
145
40%
4
6
4.18
258
20%
2
8
8.42
522
0%
0
10
Infinity
Infinity
2nd Results
% Of H2O2
Volume of H2O2 in ml
Volume of H2O in ml
Minutes
=======
Seconds
=======
100%
10
0
1.27
87
80%
8
2
1.43
103
60%
6
4
2.07
127
40%
4
6
3.59
239
20%
2
8
7.42
462
0%
0
10
Infinity
Infinity
3rd Results
% Of H2O2
Volume of H2O2 in ml
Volume of H2O in ml
Minutes
=======
Seconds
=======
100%
10
0
1.14
74
80%
8
2
1.36
86
60%
6
4
1.57
117
40%
4
6
3.32
212
20%
2
8
6.30
390
0%
0
10
Infinity
Infinity
Average Results
% Of H2O2
Volume of H2O2 in ml
2-ethyl-1,3-hexanediol. The molecular weight of this compound is 146.2g/mol. It is converted into 2-ethyl-1-hydroxyhexan-3-one. This compounds molecular weight is 144.2g/mol. This gives a theoretical yield of .63 grams. My actual yield was .42 grams. Therefore, my percent yield was 67%. This was one of my highest yields yet. I felt that this was a good yield because part of this experiment is an equilibrium reaction. Hypochlorite must be used in excess to push the reaction to the right. Also, there were better ways to do this experiment where higher yields could have been produced. For example PCC could have been used. However, because of its toxic properties, its use is restricted. The purpose of this experiment was to determine which of the 3 compounds was formed from the starting material. The third compound was the oxidation of both alcohols. This could not have been my product because of the results of my IR. I had a broad large absorption is the range of 3200 to 3500 wavenumbers. This indicates the presence of an alcohol. If my compound had been fully oxidized then there would be no such alcohol present. Also, because of my IR, I know that my compound was one of the other 2 compounds because of the strong sharp absorption at 1705 wavenumbers. This indicates the presence of a carbonyl. Also, my 2,4-DNP test was positive. Therefore I had to prove which of the two compounds my final product was. The first was the oxidation of the primary alcohol, forming an aldehyde and a secondary alcohol. This could not have been my product because the Tollen’s test. My test was negative indicating no such aldehyde. Also, the textbook states that aldehydes show 2 characteristic absorption’s in the range of 2720-2820 wavenumbers. No such absorption’s were present in my sample. Therefore my final product was the oxidation of the secondary alcohol. My final product had a primary alcohol and a secondary ketone
· I predict that the enzyme will work at its best at 37c because that
· I will take all of my results on the same day with the same
What Affects the Rate of Breakdown of Hydrogen Peroxide by Enzymes Aim = == The aim of this experiment is to find out how temperature and concentration affect the breakdown of hydrogen peroxide by an enzyme (yeast). I hope to achieve reliable results that will confirm my predictions.
The Effect of a Catalase on the Breakdown of Hydrogen Peroxide Aim To follow the progress of a catalysed reaction by measuring the volume of gas produced as the reaction proceeds. Using the initial rates of a series of experiments I will be able to find the orders of the reaction with respect to enzyme and substrate. Also to find out if concentration has an effect on the reaction when an enzyme is used to accelerate the breakdown of hydrogen peroxide.
In this case, clinical prefers that we only issue one query to get our point across because we are just verifying if the data is correct.
Within this set, the investigators randomized how many trials the participants would complete: 7, 10, or 13. Then, they were giving the chance to do 3 or 6 more trials and were ask to record their results.
Tests cannot always measure everything that needs to be measured on a course or what a st...
... performed that one day. Scores might be different on a different day, a different time, if the student’s mood is good or bad, or is tired.
To make sure it is a fair test; the procedure is repeated a couple of
I only got one set of results and therefore I wasn't able to get very
we cant just look at one variable if we want it to be a fair test
Investigating the Effect of the Enzyme Catalyse On Hydrogen Peroxide Introduction The aim of this experiment is to determine the effects of varying enzyme (catalyse) on Hydrogen Peroxide. Hydrogen Peroxide + Catalyse à Water + Oxygen 2H2O2 à H2O + O2 + Heat Apparatus & Diagram [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE] Bung Potato Hydrogen Peroxide Water Collected Oxygen Delivery Tube Measuring Cylinder [IMAGE] Using the Equipment Safely It is important that we use the apparatus carefully, as safety will be an issue throughout the whole experiment. We will wear goggles and an apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful, ensuring they don't come into contact with our eyes, skin or clothes. Catalyse is an enzyme found in all living cells.
Should such a test reveal a thin negative, some compensation can be made when developing the remainder of the roll. (Horrell 19)
...entity beyond a reasonable doubt, then neither could a second test on an even smaller sample.”