Chromatography Lab

1097 Words3 Pages
bulb-icon

Recommended: Lab. quiz chromatography

Separating Components Of A Solution Using Chromatography Introduction In this lab, solutions were separated by polarity and affinity to solids by chromatography. Chromatography is the separation of a mixture, where the components move at different rates up a medium. The medium used was chromatography paper, matched with a series of developers to aid in movement of compounds upwards. The distance moved up the paper is measured and the rf is calculated. The distance the pigments traveled is divided by the distance developer traveled. The more polar a substance the further it travels up the paper. The paper works by capillary action and absorption to separate the compounds. Mobile phase and solid phase Purpose The purpose of this lab was to …show more content…

Which of the three developers produced the best separation of the dye mixture? Why? Developer I, because it’s polarity caused farther distance traveled up the paper. Describe what happens to the original spot of plant pigments extract. Traveled up 3.5 cm from point of origin. How do your R1 values compare with those of your classmates? Similar, we worked as one group. List some other uses of chromatography. To separate liquids into individual compounds, to separate, isolate, and identify chemical compounds. Which of the 4 pigments migrated the farthest and why? Xanthophylls because they are more polar. Which of the two chlorophyll forms is more soluble? Chlorophyll a Explain why leaves change color in the fall. Because the presence of chlorophyll, which hides the carotene and xanthophyll colors, leaves. What is the function of these plant pigments in photosynthesis? To absorb light and other nutrients and convert into energy. Summary In this lab, I learned how to separate and identify chemical compounds. A skill obtained would be the use of chromatography paper to separate liquids based on solubility and affinity. This skill can be very useful in future labs to identify the chemicals in a solution and their

Show More
Related

  • Caffeine Extraction Experiment

    1054 Words  | 3 Pages

    The objective of this experiment was to perform extraction. This is a separation and purification technique, based on different solubility of compounds in immiscible solvent mixtures. Extraction is conducted by shaking the solution with the solvent, until two layers are formed. One layer can then be separated from the other. If the separation does not happen in one try, multiple attempts may be needed.

    Read More

  • biology - how light intensity affects the rate of photosynthesis

    2109 Words  | 5 Pages

    Plants can absorb and use light energy because they have a green pigment, chlorophyll, contained in the chloroplasts in some of their cells. Chlorophyll allows the energy in sunlight to drive chemical reactions. Chloroplasts act as a energy transducers, converting light energy into chemical energy. So as the plant has more light the chlorophyll inside the chloroplasts can react faster absorbing in more light for food and energy.

    Read More

  • Investigating the Effect of Light Intensity on the Size of a Plantain Leaf

    1322 Words  | 3 Pages

    They are used to produce glucose which is used as plant food and growing materials (e.g. cellulose).A leaf which is exposed to plenty of light will have sufficient amounts of food and will not need an excessive amount of chlorophyll. This enables the leaf to have a small surface area. It is also necessary for leaves in areas of high light intensity, and thus high temperature, to have small leaves to reduce the amount of transpiration. The heat will cause water to evaporate a lot faster. Leaves in shaded areas will need a large surface area full of chlorophyll to collect as much sun light as possible; essential for survival.

    Read More

  • Crayola Colors: Mixtures And Pure Substances

    740 Words  | 2 Pages

    The only noticeable difference was that it nearly traveled to the top of the filter strip with very little signs of pigment separation.

    Read More

  • Understanding Plant Responses to Light and Hormones

    666 Words  | 2 Pages

    1. In response to light, phytochrome undergoes a change in shape that leads to the activation of

    Read More

  • How Organelles Work Together

    1394 Words  | 3 Pages

    The green pigment in the chloroplasts are called chlorophyll and that is what absorbs light for a process called photosynthesis. Photosynthesis is a process to make “plant food” and it takes place in the chloroplasts. The process needs sunlight, water, oxygen and carbon dioxide to create sugar. The food created in photosynthesis helps the plant grow and survive, without it, the plant would not have energy or food to do simple tasks. Furthermore, the plant takes less oxygen and releases more oxygen during photosynthesis. In addition, like I said earlier chloroplasts have chlorophyll in them and the chlorophyll gives the plant the unique green color. To summarize, the chloroplasts are vital to plants because they create food from a process called

    Read More

  • Plant Physiology: The Shockwave Petunia Series

    1112 Words  | 3 Pages

    Pigments are chemical compounds, which reflect and absorb only certain wavelengths of visible light. Because pigments interact with light to absorb only certain wavelengths, pigments are useful to plants and other autotrophs, which make their own food through the process of photosynthesis. (Unknown author,1997).

    Read More

  • Light Intensity Essay

    1234 Words  | 3 Pages

    These plants are green because that wavelength of light is reflected instead of absorbed. Different colors of light can affect how much photosynthesis occurs, because only certain colors are absorbed. The more color that’s absorbed, the more light that’s absorbed, which leads to more photosynthesis. Our experiment demonstrated that the red wavelength was the most effective for photosynthesis behind white, which was our positive control due to the fact that it contains all the colors of the spectrum. In our final experiment for the absorption spectrum, our results supported our hypothesis and the absorbance levels decreased as we increased the wavelength.

    Read More

  • Thin Layer Chromatography Lab Report

    684 Words  | 2 Pages

    Hello student! In order to solve your problem, it is important to first understand how thin layer chromatography works. Thin Layer Chromatography is a way in which we can separate components of mixtures and identify qualities about their chemical nature. The TLC plate is made of a silica gel which plays an integral role in the distance the different samples will travel, which we will discuss later. For now, it is important to know that Silica gel is very polar. The solvent, which is what you are placing the TLC plate in, is also very important in determining the distance the spots will travel. The different properties of the mixtures, especially polarity, determine how far the spot will travel once placed in the solvent and this is what allows

    Read More

  • Identifying Five Unknown Chemicals

    1246 Words  | 3 Pages

    == Refer to, Chemistry Lab #1 – What’s the substance? I didn’t change most materials when I did this experiment, but I added 4 materials, which are: * 5 test tubes * 2 stoppers * 1 large piece of paper And I deleted 1 material, which is: * Spatula Methods = == ==

    Read More

  • Notes On Plant Pigment Chromatography

    695 Words  | 2 Pages

    3.) What type of chlorophyll does the reaction center contain? What are the roles of the other pigments?

    Read More

  • Photosynthetic Pigments Essay

    1553 Words  | 4 Pages

    The structure of chlorophyll involves a hydrophobic tail embedded in the thylakoid membrane which repels water and a porphyrin ring which is a ring of four pyrrols (C4H5N) surrounding a metal ion which absorbs the incoming light energy, in the case of chlorophyll the metal ion is magnesium (Mg2+.) The electrons within the porphyrin ring are delocalised so the molecule has the potential to easily and quickly lose and gain electrons making the structure of chlorophyll ideal for photosynthesis. Chlorophyll is the most abundant photosynthetic pigment, absorbing red and blue wavelengths and reflecting green wavelengths, meaning plants containing chlorophyll appear green. There are many types of chlorophyll, including chlorophyll a, b, c1, c2, d and f. Chlorophyll a is present in all photosynthetic organisms and is the most common pigment with the molecular formula C55H72MgN4O5. Chlorophyll b is found in plants with the molecular formula C55H70MgN4O6, it is less abundant than chlorophyll a. Chlorophyll a and b are often found together as they increase the wavelengths of light absorbed. Chlorophyll c1 (C35H30O5N4Mg) and c2 (C35H28O5N4Mg) are found in algae, they are accessory pigments and have a brown colour. Chlorophyll c is able to absorb yellow and green light (500-600nm) that chlorophyll a

    Read More

  • Photosynthesis and the Vital Role of Chlorophyll

    1362 Words  | 3 Pages

    Our world is filled with beautiful plants of all different colours. Green is clearly the most prominent colour, and we know that this is due to the presence of chlorophyll. We have also learnt that photosynthesis is a vital process that occurs in order to create glucose and oxygen, by transforming carbon dioxide and water in the presence of light energy. Chlorophyll is vital for this process due to the fact that the light energy which allows for this process to occur is trapped in the chlorophyll molecule. These molecules are situated in the chloroplasts in the mesophyll layer of leaves, but most prominently in the palisade mesophyll which is the main photosynthesising tissue. My interest was sparked when I started to think about the role this so called vital chlorophyll plays and whether it is really necessary for photosynthesis to occur to occur in plants. While thinking about this I noticed that chlorophyll leads to the green colour in plants as green is reflected by the chloroplast cells and red and blue absorbed, therefore plants which are green and white obviously will not have chlorophyll in the parts which are white, as these parts lack the green colour, meaning that photosynthesis is not supposed to occur in these areas. It is also vital to remember that leaves which are fully red still do photosynthesise but the green pigment is just masked by carotenoids, which is why only variegated leaves may be used. The most basic way to actually test this is to look along the lines of leaves which are variegated, meaning they are white and green.

    Read More

  • Paper Chromatography Essay

    1169 Words  | 3 Pages

    In this experiment, we worked with paper chromatography. Before discussing the experiment in full detail, we must first review what exactly paper chromatography is. Paper chromatography is a certain technique used for separating different mixtures. It uses a specific type of paper made up of cellulose, and has a solution that separates the mixture into the multiple substances that make up that mixture1. Paper chromatography can also test the purity of the mixtures as well2.

    Read More

  • The Importance Of Light And Visible Light

    1388 Words  | 3 Pages

    However, when plants get hit with sunlight, they utilize it. Through a process called photosynthesis, plants use light energy to make sugars and carbohydrates. Photosynthesis begins in organic molecules called pigments, which are found in plant cells’ chloroplasts. A pigment is a chemical compound that reflects certain wavelengths, which make it colorful. However, an ability more important to reflect light is to absorb certain wavelengths. Pigments are used by autotrophs, which are anything that relies solely on photosynthesis to make their own food. According to UCMP Berkeley, Pigments react within a narrow range of the visible spectrum, which makes different pigments different colors, and plants need to produce multiple types of pigments in order to absorb all the light possible. There are a few classes of pigments, but chlorophyll, carotenoids, and phycobilins are probably the most common. There are five types of chlorophylls, chlorophyll a and b being the most important. Chlorophylls are greenish molecules that contain a ring shaped stable molecule, called a porphyrin ring, which makes electrons freely move. If they move freely the ring can gain or lose electrons, and then other molecules can be provided with energized electrons. This process is how chlorophyll captures light. Another type of pigment, Carotenoids, are red, yellow, or orange colored pigments. They include the compound carotene, which is where carrots get their color. Carotenoids pass their absorbed energy to chlorophyll because they cannot directly transfer sunlight energy into the photosynthetic pathway; and because of this, they are called accessory pigments. They also play a big role in getting rid of excess light, because a big amount of sunlight often kills pigments and other photosynthetic machinery, making the plants bleach.

    Read More

More about Chromatography Lab

Open Document