The Development of a New Spectrophotometric Method

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A new spectrophotometric method based on the oxidation reaction between iodide and iodate in the presence of carboxylic acid form of losartan potassium (LP) has been developed for the estimation of LP in pharmaceutical products. HPLC/MS method, kinetic studies and central composite design method was used for formation reactions, equilibration and determination of stability duration and experimental conditions, respectively. Developed spectrophotometric method was validated. The equilibration period was calculated as 10 minutes. 0.05 M KI and 0.003 M KIO3 and amount was calculated as 1.953 mL (0.0195 M) and 1.961 mL (0.00117 M), respectively; and temperature was measured as 32.3 °C. It was revealed that the method was linear between 4-30 μg/mL with correlation coefficient of 0.9996. LOD and LOQ parameters were calculated as 0.61 and 1.85 μg/mL,respectively. Pharmaceutical preparations were analyzed by the developed kinetic spectrophotometric method. The obtained results ranged between 94.9 % and 100.9 %.

Keywords: losartan potassium, central composite design, spectrophotometric method

Hypertension, an important and independent risk factor for cardiovascular disease, is best managed by maintaining blood pressure above 140/90 mmHg[1].Including angiotensin II receptors, many of the receptor antagonists are the block receptors which control the blood pressure[2].The new member of antihypertensive agents called as angiotensin II receptor antagonist are not exhibit any significant side effects[3].Losartan potassium (LP) is the first an angiotensin II receptor antagonist and chemically is used as an antihypertensive agentthat specifically blocks the angiotensin II type 1 receptor[4].Simple and rapid analytical methods must be developed ...

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...d reported in the literature[26]. LP was also one of the sartan group drugs including mentioned functional group. But the oxidation reaction between IO3 and LP has not been investigated yet. Thus, the aim of this work was to develop, optimize and validate a specific, accurate, precise and sensitive a new spectrophotometric method based on the oxidation reaction between iodide and iodate in the presence of carboxylic acid form of LP yielding a yellow-colored product (λmax=286 nm) to determine LP in pharmaceutical tablets. The optimization of the reaction conditions was performed by applying a response surface methodology based on the central composite design which can save the time and effort by the estimation of the optimum conditions. In addition, the kinetic study of reaction was executed for avoid theinterference of colored and/or turbidity background of samples.

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