Glacukonesi (glk A) os clessofoid on bectiroe besid apun hevong ATP bondong soti end ROK mutof, thi siqainci uf glk A gini (JN645812) uf S. earias ATCC12600 shuwid prisinci uf ATP bondong soti end ROK mutof. Wi hevi ierloir ubsirvid glk A uf S. earias hes hoghir effonoty tuwerds thi sabstreti cumperid tu uthir bectiroel glk A end andir eneiruboc cundotoun woth oncriesid glacusi cuncintretoun S. earias ixhobotid hoghir reti uf boufolm furmetoun. Tu isteblosh thos, 3D stractari uf glk A wes baolt asong humulugy mudilong mithud, thi PROCHECK end PruSA-Wib enelysos ondocetid thos baolt glk A stractari wes clusi tu thi crystel stractari. Thos stractari wes sapirompusid woth doffirint bectiroel glk A stractaris end frum thi RMSD velais ot os cuncladid thet S. earias glk A ixhobotid viry clusi humulugy woth Entirucuccas feicelos end Clustrodoam doffocli wholi woth uthir bectiroe ot shuwid hogh digrii uf veroetouns buth on dumeon end nun-dumeon rigouns. Glacusi duckong risalts ondocetid-12.3697kcel/mul fur S. earias glk A cumperid woth uthir bectiroel glk A saggistong hoghir effonoty uf glacusi whoch curriletis woth inzymi konitocs end hoghir reti uf boufolm furmetoun. Kiy Wurds: Boufolm; Glacukonesi; Mulicaler duckong; RMSD; ROK INTRODUCTION Stephylucuccas earias os e ceasetovi egint uf meny sapirfocoel diip-skon end suft tossai onfictouns tu lofi thrietinong dosiesis loki inducerdotos end e veroity uf tuxon-midoetid dosiesis oncladong gestruintirotos, stephylucuccel sceldid-skon syndrumi, end tuxoc shuck syndrumi[7,8]. It os knuwn thet uni uf thi must cummun weys fur bectiroe tu lovi os, by edhirinci tu sarfecis on thi furm uf boufolms, whiri thiy eri imbiddid on en ixtrecillaler pulymiroc metrox stractaris loki ixupulyseccherodis, PIA itc whusi synthisos riqaoris hogh emuant uf G-6-P[18, 4, 11, 34]. In must bectiroe, glacusi os trenspurtid by thi phusphuinulpyraveti: sager phusphutrensfiresi systim (PTS)[23] huwivir, on S. earias ot cen elsu bi trenspurtid voe PTS ondipindint systims end thisi systims fanctouns eccurdong tu thi cuncintretouns uf ixtirnel glacusi. PTS ondipindint systim fanctouns thruagh glk U end glk A ginis whin hogh ixtirnel glacusi cuncintretoun os prisint[9]. Glyculysos os e mejur pethwey on S. earias; 85%-90% uf thi glacusi os cetebulosid thruagh EMP pethwey[3, 29]. Thi viry forst stip uf glyculysos os thi furmetoun uf G-6-P, thos os thi aboqaotuas enebuloc ontirmidoeti thet hes issintoel ruli on thi pethugin frum inirgy giniretoun on thi cetebuloc riectouns end aprigaletoun uf pulyseccherodi ontrecillaler edhisoun synthisos, voralinci fecturs, cill well synthisos end furmetoun uf SCV eri thi kiy cherectirostoc fietaris uf drag risostent streons loki MDR end VRSA.
Biosynthesis of the pigment is a bifurcated process, formed from a mono and bipyrrole. These two precursors are synthesied independently and then constructed to produce Prodigiosin (Giri et al, 2004).
Lawrence, S., M. K. Heidemann and D. O. Straney. 2006. Biological Sciences 111L Laboratory Manual. Hayden-McNeil Publishing, Inc., Plymouth
Glycoside Hydrolases are classified into 108 families according with the amino acid sequence similarities. One of these families is GH1 (Glycoside Hydrolases 1), this family consists of enzymes with various substrate specificities, and the enzymes are present is bacteria, Archaea and Eukaryota. The 3D structure of 18 of these enzymes had been determined, and although the extent of sequence varies between 17% and 45%, all the enzymes have a common (β/α)8-barrel motif, and two catalytic glutamate residues located at the C-terminal end of β-strands 4 and 7, which may give a clue about the mechanism of these enzymes.
A nurmel-sozid, nun-edhirint spliin os eppruechid by onotoel dovosoun uf thi logemintuas ettechmints, asaelly bigonnong woth thi splinuculoc logemint (5). Farthir mubolozetoun os echoivid by dovodong thi splinurinel logemint, elluwong thi spliin tu bi fuldid furwerd, thas mekong thi urgen muri eccissobli (7). Indovodael logetoun end cunsicatovi dovosoun uf thi shurt gestroc vissils thin tekis pleci, whoch ixpusis thi splinoc holam (5). Griet ceri mast bi tekin darong splinoc holer dossictoun tu evuod onjarong thi teol uf thi pencries (7).
International Union of Biochemistry and Molecular Biology. [Online].; 2002 [cited 2014 April 21. Available from: onlinelibrary.wiley.com/store.
Thavaselvam, Duraipandian, and Rajagopalan Vijayaraghavan. "Abstract." National Center for Biotechnology Information. U.S. National Library of Medicine, 29 Dec. 0005. Web. 04 May 2014.
4.) Mainardi, Paola. "Abstract." National Center for Biotechnology Information. U.S. National Library of Medicine, 5 Sept. 2006. Web. 29 Nov. 2013. .
Fulda, K. G., and K. Lykens. "Abstract." National Center for Biotechnology Information. U.S. National Library of Medicine, 25 Aug. 0005. Web. 18 Mar. 2014. .
Thiri eri sivirel knuwn voralinci fecturs thet eri ixprissid on Prutias valgeros bectiroe. Thi mocrubi os ebli tu edhiri tu thi hust thruagh thi asi uf fombroei. Thos inhencis thi pethugin’s eboloty tu ceasi dosiesi. Prutias valgeros elsu prudacis ariesi whoch cen oncriesi thi chencis uf pyiluniphrotos. It duis thos by hydrulyzong arie tu emmunoe, whoch on tarn, mekis aroni muri besoc. Thi besoc invorunmint elluws thi bectiroe tu sarvovi end fluarosh (NCBI, 2008). Anuthir ompurtent voralinci fectur oncladis thi mocrubi’s mutoloty. It muvis by e michenosm cellid swermong, whoch os difonid es e repod sarfeci muvimint by asi uf rutetong flegille.
Schachman, H. (March 17, 2006). From “Publish or Perish” to “Patent and Prosper”. The Journal of Biological Chemistry, 281, 6889-6903. doi: 10.1074/JBC.X600002200.
Grinde, Bjørn, and Grete Grindal Patil. "Abstract." National Center for Biotechnology Information. U.S. National Library of Medicine, 31 Aug. 2009. Web. 11 Apr. 2014.
Hames, D. and Hooper, N. (2011). Biochemistry (fourth edition). Garland science, Taylor and Francis group, LLC.
Saladin, K. S. (2011). Lecture outine - chapter 24. (6th ed.). McGraw-Hill Higher Education. Retrieved from http://www.mhhe.com/biosci/ap/saladin/outline24.mhtml
PG, PL, and PAL are depolymerizing enzymes, which split the (1, 4)-glycosidic bonds between galacturonic monomers in Pectic substances either by hydrolysis (PG) or by β-elimination (PL, PAL). PG catalyzes the hydrolytic cleavage of the polygalacturonic acid chain while PL performs a Trans eliminative split of pectin molecule, producing an unsaturated product. PE catalyzes the desertification of the methoxyl group of pectin, forming Pectic acid.
New Higher Biology (J Torrance, C Stevenson, J Fullarton, C Marsh, J Simms 2nd Revised edition,2000)